Histochemical Test of root, petiole and leaf of Kelembak (Rheum officinale Baill.)

Abstract

Kelembak (Rheum officinale Baill.) is perennial shrub with pharmacological activities, and well-known as one of traditional herbal medicine used widely in the world. The phytochemical study, as well as histochemical test, is pivotal to determine the potency of herbs used for disease treatment. Based on its secondary metabolite content. Histochemical test of R.officinale vegetative organs had not been reported. This study aims to determine the content of compounds in the vegetative organs of kelembak and provide information on the anatomical position of each compound in root, petiole and leaf. The preparation of histochemical test used freehand section method. Each sample was made in three replications for this test. A total of four secondary metabolites were examined in this study, i.e. alkaloid, tannin, lipid and flavonoid. The positive content of each compound was indicated by the coloration change. The specimens were then observed and photographed using light microscope. The results showed that the root, petiole and leaf showed positive content of alkaloids, tannins, lipids and flavonoids. However, the anatomical position of each secondary metabolites are different among root, petiole and leaf. The results of this study provide the first information of histochemistry vegetative organs of Rheum officinale.

Potential of Leaf Extracts Sonneratia alba and Avicennia alba as a Biolarvacide of Aedes aegypti Mosquito

“…Several bioactive compounds, such as alkaloids, phenolic compounds, tannins, lipids, and flavonoids, are stored in sponge tissue. 16 The research results show that the more layers of sponges on a leaf, the more potential there is for bioactive compounds such as biolarvacides.…”

“…15 These organelles are abundant in the mesophyll tissue, especially the spongy tissue, which functions as a storage place for metabolic products and by-products. 16 Based on this, it can be that the thicker the spongy tissue of a leaf, the more bioactive compounds it contains. Based on the results of this study, it was found that the potential as a larvicidal bioactive compound of A. alba was superior to that of S. alba.…”

Anatomical Structure and Histochemical Study Male Strobilus of Melinjo (Gnetum gnemon L.)

“…Uji histokimia dilakukan pada preparat segar nodus dan internodus yang dipotong melintang, serta bunga jantan yang dipotong membujur. Setiap potongan dari nodus, internodus, dan bunga jantan kemudian diuji menggunakan berbagai reagen untuk masingmasing golongan senyawa metabolit sekunder, yaitu fenolik (10% FeCl3 dan larutan Na2CO3), terpenoid (CuSO4 5%), flavonoid (5% NaOH), alkaloid (reagen Dragendoff), tanin (10% FeCl3) (Rahayu dkk., 2021;Mulyani & Laksana, 2011;(Ergina dkk., 2014;Nurhasanah & Iriani, 2021).…”

Distribution and FTIR-based fingerprint of secondary metabolites in different organs of ant-plant (Myrmecodia tuberosa)

“…Plant secondary metabolites are generally accumulated in specialized structures called secretory structures (Lange 2015). Each part of a plant has a different distribution and types of secondary metabolite (Nurhasanah and Iriani 2021;Umar et al 2021a). The anatomical characteristics and secretory structures of medicinal plants need to be validated to ensure authenticity and identify the highest accumulation of a bioactive substance (Coelho et al 2012).…”