Histochemical study of fibrillar proteins of the extracellular matrix in benign and malignant mammary neoplasms in dogs

Martins, Ana Maria Cristina Rabello Pinto da Fonseca; Tamaso, Elia; Guerra, José Luiz

doi:10.1590/s1413-95962002000100008

Cited by 9 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4 , silver-stained sections of tumors [ 25 ] consistently displayed strong and highly organized tissue proliferation surrounding and slitting the original neoplastic mass into smaller tumor islands. This localized desmoplastic reaction is due to the vaccination because it is not observed in retrospective review of dozens of untreated dogs with mammary adenocarcinoma (unpublished data and ref [ 26 ] ). However, it remains to be revealed how a thick layer of stroma surrounding a tumor contributes to prevention of the tumor growth and spreading.…”

Section: Resultsmentioning

confidence: 99%

Pilot study of p62 DNA vaccine in dogs with mammary tumors

Gabai¹,

Venanzi

Bagashova³

et al. 2014

Oncotarget

View full text Add to dashboard Cite

Our previous data demonstrated profound anti-tumor and anti-metastatic effects of p62 (sqstm1) DNA vaccine in rodents with various types of transplantable tumors. Testing anti-cancer medicine in dogs as an intermediary step of translational research program provides two major benefits. First, clinical data collected in target animals is required for FDA/USDA approval as a veterinary anti-cancer drug or vaccine. It is noteworthy that the veterinary community is in need of novel medicine for the prevention and treatment of canine and feline cancers. The second more important benefit of testing anti-cancer vaccines in dogs is that spontaneous tumors in dogs may provide invaluable information for human trials. Here, we evaluated the effect(s) of p62 DNA vaccine on mammary tumors of dogs. We found that p62 DNA vaccine administered i.m. decreased or stabilized growth of locally advanced lesions in absence of its overall toxic effects. The observed antitumor activity was associated with lymphocyte infiltration and tumor encapsulation via fibrotic reaction. This data justifies both human clinical trials and veterinary application of p62 DNA vaccine.

show abstract

Section: Resultsmentioning

confidence: 99%

Pilot study of p62 DNA vaccine in dogs with mammary tumors

Gabai¹,

Venanzi

Bagashova³

et al. 2014

Oncotarget

View full text Add to dashboard Cite

show abstract

“…(A) percentage of fine fibers according to analysis times (ANOVA, "a": p <0.05 in relation to the saline group; "b": p <0.05 in relation to extract/hydrogel group); (B) Percentage of thick fibers according to analysis times (ANOVA, "a": p <0.05 in relation to the collagenase group; "b": p <0.05 in relation to extract/hydrogel group). yellow, with an intense birefringence (Martins et al, 2002). The higher amount of thick fibers than fine fibers in all groups characterizes wound maturation.…”

Section: In Vivo Healing Evaluationmentioning

confidence: 87%

Efficacy of application of Equisetum pyramidale Goldm. hydrogel for tissue restoration of induced skin lesions in Wistar rats

et al. 2020

View full text Add to dashboard Cite

Abstract Medicinal plants, such as E. pyramidale, are alternatives against infections and assist in the healing process of wounds. We evaluated the effects of Hydrogel of E. pyramidale on the healing of cutaneous wounds in animal models by morphological, morphometric and histological analyses, and elucidated major components. The ethanolic extract of E. pyramidale shoots was submitted to phytochemical analysis. For cicatrizing activity assay, Wistar rats were used, and the groups evaluated were hydrogel containing ethanolic extract of E. pyramidale at 2% compared to control groups. The treatment periods were 3, 7 and 21 days, and macroscopic and microscopic analyses were performed. Data were submitted to Analysis of Variance (p <0.05). Phytochemistry and quantification evidenced that flavonoids are main constituents and that they were evidenced by thin layer chromatography (TLC), high efficiency (HPLC) and infrared (IR) methods. In TLC, using flavonoid patterns, we observed pinocembrin. The hydrogel containing ethanolic extract of E. pyramidale at 2% was effective in wound regression. E. pyramidale can be used for the treatment of second intention wounds, and the effective healing may be due to a high flavonoid content.

show abstract

“…Martins in their study using PSR-stained collagen fibers on benign and malignant mammary neoplasms in dogs observed that in comparison to benign neoplasms, carcinomas exhibited irregular and randomly distributed collagen fibers in their stroma which partially or discontinuously surrounded the neoplastic area. [ 14 ] Similarly, Martins et al . in their study on OSCC observed that in a lower grade of malignancy (i.e., well-differentiated OSCC), collagen fibers near invasive front were arranged parallel to the tumor islands.…”

Section: Discussionmentioning

confidence: 98%

Stromal fibers in oral squamous cell carcinoma: A possible new prognostic indicator?

Kardam¹,

Mehendiratta²,

Rehani³

et al. 2016

J Oral Maxillofac Pathol

View full text Add to dashboard Cite

Background:Many studies have been carried out to study the role of extracellular matrix proteins, growth factors and matrix metalloproteinases on tumor invasion. However, literature related to the analysis of connective tissue fibers in varying grades of oral squamous cell carcinoma (OSCC) is very limited.Aim:To analyze the changes in collagen and elastic fibers in varying grades of (OSCC).Settings and Design:This retrospective study was carried out using a light and polarizing microscope.Materials and Methods:Three sections each were cut from fifty samples of varying grades of OSCC and ten samples of control followed by staining with H and E, Picrosirius-Red and Verhoeff–Van Gieson. Qualitative and quantitative analysis of collagen and elastic fibers were accomplished using set criteria.Statistical Analysis:Data were entered into the Statistical Package for Social Sciences (SPSS) version 13.5 for analysis.Results:A change in colors of collagen fibers was seen on progressing from well to poorly differentiated OSCC. Thin collagen fibers predominantly exhibited greenish yellow, but the thick fibers exhibited a variety of colors. As the grade of OSCC progressed, collagen fibers were loosely packed haphazardly arranged. Statistically insignificant results were obtained for quantitative analysis of collagen and qualitative analysis of elastic fibers.Conclusion:The collagen fibers undergo a change in color, orientation and packing in the stroma of varying grades of OSCC. The uniqueness of this study lies in the exploration of elastic fibers in OSCC which has not been done so far.

show abstract

Histochemical study of fibrillar proteins of the extracellular matrix in benign and malignant mammary neoplasms in dogs

Cited by 9 publications

References 0 publications

Pilot study of p62 DNA vaccine in dogs with mammary tumors

Pilot study of p62 DNA vaccine in dogs with mammary tumors

Efficacy of application of Equisetum pyramidale Goldm. hydrogel for tissue restoration of induced skin lesions in Wistar rats

Stromal fibers in oral squamous cell carcinoma: A possible new prognostic indicator?

Contact Info

Product

Resources

About