Histochemical studies of wound healing in the hair-loss mouse

Abstract

Summary. Adult female hair-loss (hl) mice (Mus musculus) were given incised wounds through the full thickness of the dorsal skin. At specified intervals thereafter (i. e., 1, 2, 4, 6, 12, and 20 hours), the animals were sacrificed and the wound sites were excised, processed, and sectioned: Enzyme and carbohydrate histochemical procedures were carried out on frozen and paraffin sections respectively. Nonspecific esterase was demonstrated in one hour wounds and a slight increase was observed through six hours. No other enzyme histochernical procedure gave a satisfactory reaction in the wound site. The Periodic Acid-Schiff reaction for carbohydrates revealed a steadily increasing reaction from one to six hours and a decrease in intensity by twelve hours. The causative agent for the PAS reaction may be serum glycoproteins transported to the wound site as part of the inflammatory process.

Informative M�glichkeiten der Wundoberfl�che f�r Vitalit�tsbeweis und Wundaltersbestimmung bei Hautschnittwunden

“…Gleichzeitig wird festgestellt, dab das Erseheinen und die Entwicklung der h~imodynamischen Ver/inderungen und besonders der Leukozyteninfiltration in der peripheren Zone wesentliche Zeitvariationen aufweisen. Wie AllgOwer (1967) (Raekallio 1961(Raekallio , 1966(Raekallio , 1967Friebel und Woohsmann 1968;Lindner 1969;Berg et al 1976Berg et al , 1977Kontzevich et al 1977;Hancock et al 1978;Bode et al 1979 u.a.). Deshalb empfehlen die meisten yon ihnen und insbesondere Raekallio (1972), dab die Wundaltersbestimmung durch die Anwendung mehrerer unabh~ngiger Methoden erfolgt.…”