Histochemical studies of the extracellular matrix of human articular cartilage—a review

Hyllested, Jacob Legaard; Veje, K; Østergaard, Keld

doi:10.1053/joca.2002.0519

Cited by 68 publications

(44 citation statements)

References 95 publications

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“…Short T2* water fraction parallels changes are not considered (18). In addition, the Mankin grading system includes as a criterion safranin O staining intensity, which is affected by protocol variability, such as tissue fixation and decalcification, and these factors could lead to errors in the measure (39,40). Fourth, there is a discrepancy in the image scales between histologic sections that are a few micrometers in thickness and MR images that are several millimeters thick.…”

Section: Discussionmentioning

confidence: 99%

Ultrashort–Echo Time MR Imaging of the Patella with Bicomponent Analysis: Correlation with Histopathologic and Polarized Light Microscopic Findings

Pauli¹,

Bae²,

Lee³

et al. 2012

Radiology

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Purpose:To correlate short and long T2* water fractions, derived from ultrashort-echo time (TE) magnetic resonance (MR) imaging, with semiquantitative histopathologic and polarized light microscopic (PLM) assessment of human cadaveric patellae cartilage. Materials and Methods:Twenty human cadaveric patellae were evaluated by using ultrashort-TE imaging, spin-echo imaging, histopathologic analysis, and PLM, with institutional review board approval. Short and long T2* water components were evaluated for each patella by using bicomponent fitting of ultrashort-TE signal decay. Four to six regions of interest (ROIs) within each patella were chosen for correlation between ultrashort-TE bicomponent analysis, histopathologic grading (Mankin score), and PLM grading (Vaudey score). Results:Ultrashort-TE imaging with bicomponent analysis showed two distinct water components with a short T2* and a longer T2* in all patellae. ROI analysis showed that the short T2* fraction was correlated significantly with the Mankin (r = 0.66, P , .001) and Vaudey (r = 0.68, P , .001) scores. The Mankin scores were weakly positively correlated with T2 (r = 0.28, P = .13) and short T2* (r = 0.24, P = .14) but were negatively correlated with long T2* (r = 20.55, P , .01). The Vaudey scores were weakly positively correlated with T2 (r = 0.18, P = .16) and short T2* (r = 0.22, P = .14) but were negatively correlated with long T2* (r = 20.55, P , .01). Conclusion:Short T2* water fraction derived from ultrashort-TE imaging with bicomponent analysis correlates significantly with both the Mankin and Vaudey scores and may serve as a biomarker of cartilage degeneration.q RSNA, 2012

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Section: Discussionmentioning

confidence: 99%

Ultrashort–Echo Time MR Imaging of the Patella with Bicomponent Analysis: Correlation with Histopathologic and Polarized Light Microscopic Findings

Pauli¹,

Bae²,

Lee³

et al. 2012

Radiology

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“…Thus, the Safranin O-Fast Green staining protocol has been widely used as a marker for PG loss. 28 After staining, the AC slices were rinsed in 95% ethanol and mounted in slides. All histological sections were automatically imaged by a bright-field digital slide scanner with a 20× dry objective (ScanScope AT, Aperio, Vista, CA).…”

Section: Histology Assessmentmentioning

confidence: 99%

Depth-dependent refractive index of normal and early degenerated articular cartilage

Wang¹,

Wu²,

Kirk³

2013

J. Biomed. Opt

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Abstract. The depth distributions of refractive index (RI) in normal and early degenerated articular cartilage (AC) were measured and correlated to the pathological statuses. Utilizing a confocal microscope, the depth distribution of RI was determined with an interval of 50 μm from the articular surface and approximated as a smooth curve. The fluctuation of RI was quantified with a specially defined coefficient and the proteoglycan (PG) loss was quantified with histological images. The overall RI of the AC collected from the femoral condyles of mature sheep was 1.4444. Significant differences existed in the refractive indices of different cartilage samples or even in the different depths of the same sample. The RI of normal cartilage distributed as a regular ramp or arch curve with depth and peaked in the middle-deep zone. The cartilage samples detected with high PG losses were coupled with severe fluctuations of RI. The fluctuating level was increased with pathological progress. The coefficient of RI fluctuation can distinguish the normal cartilage from the initially degenerated one, which potentially provides an indicator to detect the pathological development of AC. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…The OCT embedding material was removed from the FTIRI samples by adding a few drops of de-ionized water and rapid removal with a pipette prior to air-drying the sample. Histological samples were fixed in neutral buffered formalin and stained with Safranin-O (Hyllested et al, 2002) and all specimens were evaluated histologically for OA severity.…”

Section: Human Cartilage Specimensmentioning

confidence: 99%

Fourier transform infrared imaging of focal lesions in Human osteoarthritic cartilage

David-Vaudey

Burghardt²,

Keshari³

et al. 2005

eCM

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Fourier Transform Infrared Imaging (FTIRI) is a new method for quantitatively assessing the spatial-chemical composition of complex materials. This technique has been applied to examine the feasibility of measuring changes in the composition and distribution of collagen and proteoglycan macromolecules in human osteoarthritic cartilage. Human cartilage was acquired post-operatively from total joint replacement patients. Samples were taken at the site of a focal lesion, adjacent to the lesion, and from relatively healthy cartilage away from the lesion. Sections were prepared for FTIRI and histochemical grading. FTIRI spectral images were acquired for the superficial, intermediate, and deep layers for each sample. Euclidean distance mapping and quantitative partial least squares analysis (PLS) were performed using reference spectra for type-II collagen and chondroitin 6-sulphate (CS6). FTIRI results were correlated to the histology-based Mankin scoring system. PLS analysis found relatively low relative concentrations of collagen (38 ± 10%) and proteoglycan (22 ± 9%) in osteoarthritic cartilage. Focal lesions were generally found to contain less CS6 compared to cartilage tissue adjacent to the lesion. Loss of proteoglycan content was well correlated to histological Mankin scores (r=0.69, p<0.0008). The evaluation of biological tissues with FTIRI can provide unique quantitative information on how disease can affect biochemical distribution and composition. This study has demonstrated that FTIRI is useful in quantitatively assessing pathology-related changes in the composition and distribution of primary macromolecular components of human osteoarthritic cartilage.

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Histochemical studies of the extracellular matrix of human articular cartilage—a review

Abstract: Detailed studies of fixation and staining procedures should be carried out and standards for cartilage sampling, handling and evaluation agreed upon if results from different laboratories are to be compared.

Cited by 68 publications

References 95 publications

Ultrashort–Echo Time MR Imaging of the Patella with Bicomponent Analysis: Correlation with Histopathologic and Polarized Light Microscopic Findings

Ultrashort–Echo Time MR Imaging of the Patella with Bicomponent Analysis: Correlation with Histopathologic and Polarized Light Microscopic Findings

Depth-dependent refractive index of normal and early degenerated articular cartilage

Fourier transform infrared imaging of focal lesions in Human osteoarthritic cartilage

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