Histochemical studies of the colonic epithelial glycoproteins of the normal rabbit

Reid, P. E.; Walker, David C.; Terpin, T; Owen, David A.

doi:10.1007/bf01002608

Cited by 14 publications

(4 citation statements)

References 56 publications

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“…The absence of DBA and UEA-I binding is in agreement with results of previous studies of chicks [23], which have shown that caecal goblet cells are devoid of GalNAc and h-L-fucose residues. Our finding of changes in PAS reactive neutral mucins and AB reactive carboxylated and sulphated mucins in goblet cells during upward migration along the crypts in the chick intestinal tract are consistent with studies in the mouse [25], rat [4,26] and rabbit [27], where similar changes associated with the position of goblet cells in intestinal crypts have been described. Coincident with the histochemical changes, the WGA binding of the large intestine was increased along the crypt-surface axis, although this pattern was not reflected in the small intestine or the caecum.…”

Section: Discussionsupporting

confidence: 80%

The influence of diet on the mucin carbohydrates in the chick intestinal tract

Sharma¹,

Fernandez

Hinton

et al. 1997

CMLS, Cell. mol. life sci.

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The presence of nutrients in the intestinal lumen is a major factor influencing bacterial colonization in poultry. This study was conducted to investigate the effects of poultry feed on viscosity of intestinal contents and on mucin carbohydrates by comparing jejunal supernatants and the histochemical composition of goblet cells in chicks reared to 5 weeks of age on either a conventional maize-based diet or a wheat-based diet or a wheat diet supplemented with 0.1% xylanase. Regional differences in the distribution of the neutral, carboxylated and sulphated mucins were demonstrated using conventional histochemical techniques, while a panel of lectins was used to study alterations in glycoconjugate synthesis of mucins in chicks fed different diets. 1) Feeding a diet supplemented with xylanase lowered the viscosity but increased the amount of neutral, carboxylated and sulphated mucins in the jejunum. 2) In chicks fed a maize-based diet, neutral mucins increased in the surface and upper crypt goblet cells of the small and large intestines but decreased in the caecum. 3) Feeding a diet supplemented with xylanase modified crypt-surface glycosylation of N-acetylglucosamine residues and resulted in loss of sialic acid residues in the small and large intestines. These results indicate that the constituents of poultry feed, in particular the consumption of a diet supplemented with xylanase, lead to changes in intestinal viscosity and mucin composition which are associated with alterations in the goblet cell glycoconjugates of the chick intestinal tract.

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Section: Discussionsupporting

confidence: 80%

The influence of diet on the mucin carbohydrates in the chick intestinal tract

Sharma¹,

Fernandez

Hinton

et al. 1997

CMLS, Cell. mol. life sci.

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“…A constituent that we found in the oesophageal mucins was chondroitin. This non-sulphated glycosoaminoglycans, substituting for sialic acid and hyaluronic acid, may play a role in defensive mechanisms, resisting the action of bacterial neuraminidases and hyaluronidases that digest sialic and hyaluronic acid but not chondroitin (Reid et al, 1988;Scocco et al, 1997). Conversely, sialomucins were demonstrated in adult U. cirrosa (Pedini et al, 2001) and in other teleostean species (Murray et al, 1996;Reifel and Travil, 1997).…”

Section: Discussionmentioning

confidence: 99%

Complex Carbohydrates Occurring in the Digestive Apparatus of Umbrina Cirrosa (L.) Fry

2004

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The glycosoaminoglycans in the digestive apparatus of immature fish have important biological functions and are involved in morphofunctional differentiation. The aim of this study was to investigate the glycoconjugate histochemistry in the different parts of the digestive apparatus (oesophagus, stomach, intestine) of Umbrina cirrosa (L.) fry using classical histochemical reactions (periodic acid-Schiff, Alcian blue pH 2.5, Alcian blue/periodic acid-Schiff, high iron diamine, low iron diamine) in conjunction with glycolytic digestions that degrade different classes of glycosoaminoglycans. No differences were observed in the reactivity to conventional histochemical staining of the oesophagus, stomach or intestine among 27-, 34- or 44-day-old fry. In the oesophagus, the mucopolysaccharides contained chondroitin sulphates B and A and/or C, heparan sulphate and chondroitin. In the stomach, only neutral glycoconjugates were revealed, whereas in the intestine there were only chondroitin sulphates. Some differences in the type and content of glycoconjugates were found in Umbrina cirrosa (L.) fry compared to those of adult subjects, probably related to different dietary habits and to changes in the environmental conditions.

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“…Glycoproteins with O ‐acyl sugars elaborated by, mucous goblet cells in the epithelia of lips and associated structures of G. lamta have also been identified in the mucous cells of rat, rabbit and human colon (Park et al., 1987a; Reid et al., 1988a,b, 1990; Reid and Park, 1990). Park et al.…”

Section: Discussionmentioning

confidence: 99%

Glycoproteins in the Epithelium of Lips and Associated Structures of a Hill Stream Fish Garra lamta (Cyprinidae, Cypriniformes): A Histochemical Investigation

Pinky

Mittal²,

Mittal³

2007

Anat Histol Embryol

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A series of histochemical procedures were employed to localize and characterize glycoprotein (GP) classes elaborated in the epithelia of the upper and lower lips and associated structures, namely the rostral cap, the adhesive pad, the horny upper and lower jaw sheaths and the folds of skin between them, of a hill stream fish Garra lamta. The epithelia of the lips, the folds of skin and the major portions of the rostral cap and the adhesive pad are mucogenic. The epithelia of the horny jaw sheaths and parts of the rostral cap and the adhesive pad are keratinized. Based on the histochemical characterization of GPs, the cells involved in the secretions in the epithelia at the mucogenic regions of the rostral cap and the adhesive pad comprise the epithelial cells, the type A mucous cells and the club cells. In the lips and the folds of skin, in contrast, the club cells are absent and most mucous cells belong to the type B category. Type A mucous cells are few. GPs elaborated by cellular components of the mucogenic epithelia include GPs with oxidizable vicinal diols, GPs with O-sulphate esters, GPs with sialic acid residues without O-acyl substitution or with O-acyl substitution at C7, C8 or C9 and GPs with O-acyl sugars. The different types of cells show significant differences in the classes as well as in the concentrations of the GPs elaborated by them. GPs have also been identified in the subcorneal space between the unculi and the epithelial cells in the replacement layer in the epithelia at the keratinized regions. Elaboration of more than one type of GPs suggests a basis for functional discrimination in their role in the mucous secretions at the surface as an adaptation to the feeding ecology and the environment inhabited by the fish.

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Histochemical studies of the colonic epithelial glycoproteins of the normal rabbit

Cited by 14 publications

References 56 publications

The influence of diet on the mucin carbohydrates in the chick intestinal tract

The influence of diet on the mucin carbohydrates in the chick intestinal tract

Complex Carbohydrates Occurring in the Digestive Apparatus of Umbrina Cirrosa (L.) Fry

Glycoproteins in the Epithelium of Lips and Associated Structures of a Hill Stream Fish Garra lamta (Cyprinidae, Cypriniformes): A Histochemical Investigation

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