Histochemical properties of sulfated glycoconjugates in developing enameloid matrix of the fish Polypterus senegalus

Kogaya, Yasutoku

doi:10.1007/bf00490130

Cited by 14 publications

(3 citation statements)

References 33 publications

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“…The presence of heparan suifate has also been recognized as a component in the gingival epithehum (3,8,11). Our previous studies (14,43) confirmed that HID-TCH-SP stain deposits in the dental basal lamina of rat and fish {Polypterus senegalus) are heparan suifate proteoglycans. Although in the present study we have not examined the presice histochemical properties of sulfated giycosaminogiycans, from previous studies it is highly suggestive that the stain deposits associated with the basement membranes of the junctional epithelium represent heparan suifate, Heparan suifate is capable of interaction with a variety of matrix macromolecules such as collagen, fibronectin, or laminin and serves in cell-cell recognition, attachment, and cell-matrix binding (17,20).…”

Section: Discussionsupporting

confidence: 59%

Histochemical localization at the electron microscopic level of sulfated glycosaminoglycans in the rat gingiva

Kogaya

Haruna

Vojinović

et al. 1989

J of Periodontal Research

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Using the high iron diamine thiocarbohydrazide silver proteinate (HID-TCH-SP) staining technique, we investigated ultrastructural localization of sulfated glycosaminoglycans (GAGs) in the rat gingiva shortly after eruption, especially those associated with internal and external basal laminae. In the apical portion of the internal basal lamina, HID-TCH-SP stain deposits were distributed mainly in the region of the lamina lucida located between the lamina densa and the distal surface membrane of the junctional epithelium and inside the depression of the distal surface membrane adjacent to the basal lamina. Stain deposits were also detected on the surface membrane of the cytoplasmic protrusion. Interestingly, the density of HID-TCH-SP stain deposits in the internal basal lamina was highest in the apical portion of the junctional epithelium and decreased in the coronal direction, finally tending to disappear completely. On the other hand, in the external basal lamina the deposits were localized in the whole region of the basal lamina or at both sites of the lamina densa. HID-TCH-SP stain deposits were also detected external to the lamina densa in the basement membrane associated with capillaries and in the connective tissue where they were distributed in close relation to collagen fibrils. Testicular hyaluronidase digested most HID-TCH-SP stain deposits in the connective tissue, whereas those in the region of basement membranes resisted this enzymatic digestion.

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Section: Discussionsupporting

confidence: 59%

Histochemical localization at the electron microscopic level of sulfated glycosaminoglycans in the rat gingiva

Kogaya

Haruna

Vojinović

et al. 1989

J of Periodontal Research

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“…Enamel covers the tooth surface in various tetrapods, whereas enameloid is common in chondrichthyans and actinopterygians (Sire et al, ). Enameloid is different from enamel in developmental origin (Poole, ); the enameloid ECM is thought to be secreted principally by odontoblasts in chondrichthyans (Sasagawa, ) and by both odontoblasts and inner dental epithelial cells (corresponding to ameloblasts) in actinopterygians (Shellis and Miles, ; Kogaya, ; Kawasaki and Weiss, ).…”

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confidence: 99%

SCPP genes in the coelacanth: Tissue mineralization genes shared by sarcopterygians

Kawasaki

Amemiya

2013

J Exp Zool Pt B

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The coelacanth is the basal-most extant sarcopterygian that has teeth and tooth-like structures, comprising bone, dentin, and enamel or enameloid. Formation of these tissues involves many members of the secretory calcium-binding protein (SCPP) family. In tetrapods, acidic-residue-rich SCPPs are used in mineralization of bone and dentin, whereas Pro/Gln-rich SCPPs participate in enamel formation. Teleosts also employ many SCPPs for tissue mineralization. Nevertheless, the repertoire of SCPPs is largely different in teleosts and tetrapods; hence, filling this gap would be critical to elucidate early evolution of mineralized tissues in osteichthyans. In the present study, we searched for SCPP genes in the coelacanth genome and identified 11, of which two have clear orthologs in both tetrapods and teleosts, seven only in tetrapods, and two in neither of them. Given the divergence times of these vertebrate lineages, our discovery of this many SCPP genes shared between the coelacanth and tetrapods, but not with teleosts, suggests a complicated evolutionary scheme of SCPP genes in early osteichthyans. Our investigation also revealed both conserved and derived characteristics of SCPPs in the coelacanth and other vertebrates. Notably, acidic SCPPs independently evolved various acidic repeats in different lineages, while maintaining high acidity, presumably important for interactions with calcium. Furthermore, the three Pro/Gln-rich SCPP genes, required for mineralizing enamel matrix and confirmed only in tetrapods, were all identified in the coelacanth, strongly suggesting that enamel is equivalent in the coelacanth and tetrapods. This finding corroborates the previous proposition that true enamel evolved much earlier than the origin of tetrapods.

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“…This method has previously been described in detail [Kogaya, 1989]. In brief, the specimens were placed in HID solution for 18 h, rinsed several times in distilled water, postfixed in 2% osmium tetroxide in 0.1 M sodium cacodylate buffer (pH 7.4), dehydrated and embedded in Taab 812 resin.…”

Section: Methodsmentioning

confidence: 99%

The Ganoine Membrane of the Scales of <i>Polypterus senegalus</i> Includes a Labyrinth-Like Structure

Kogaya

Kubo²,

Iwaku³

2001

Cells Tissues Organs

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The ganoine membrane is a basement membrane-like structure appearing between the maturation stage inner ganoine epithelium (IGE) and mineralized ganoine of polypterids and lepisosteids. In the present study, further ultrastructural investigation of the ganoine membrane of Polypterus senegalus was made and it was confirmed that it possessed a specialized labyrinth-like structure. The complete ganoine membrane was composed of six distinct zones: (1) a filamentous zone facing the distal plasmalemma of IGE, (2) lamella zone I, (3) vertical striation zone, (4) lamella zone II, (5) electron-dense zone, (6) electron-lucent zone directly facing the maturing ganoine surface. The ganoine membrane sometimes partially lacked some of these zones, but the electron-dense zone was usually present. Oblique or horizontal sections of the membrane showed that the vertical striation zone was made up of a typical labyrinth-like structure. Certain sulfated glycoconjugates were localized at the filamentous zone, especially adjacent to the lamella zone I and over the electron-dense zones.

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Histochemical properties of sulfated glycoconjugates in developing enameloid matrix of the fish Polypterus senegalus

Cited by 14 publications

References 33 publications

Histochemical localization at the electron microscopic level of sulfated glycosaminoglycans in the rat gingiva

Histochemical localization at the electron microscopic level of sulfated glycosaminoglycans in the rat gingiva

SCPP genes in the coelacanth: Tissue mineralization genes shared by sarcopterygians

The Ganoine Membrane of the Scales of <i>Polypterus senegalus</i> Includes a Labyrinth-Like Structure

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