Histochemical Observations on the Lipid Changes in the Rat Corpus Luteum During Various Reproductive States

Guraya, Sardul S.

doi:10.1530/jrf.0.0420059

Cited by 12 publications

(18 citation statements)

References 24 publications

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“…After the hormonal treatments, the females were killed and their ovaries were processed according to various histochemical methods for lipids and cholesterol and/or esters as described by Guraya (1975). The lipid changes described previously in detail for the CL of normal (or untreated) rats during the oestrous cycle, pregnancy and lactation (Guraya, 1975) constituted the control observations.…”

Section: Methodsmentioning

confidence: 99%

“…The lipid changes described previously in detail for the CL of normal (or untreated) rats during the oestrous cycle, pregnancy and lactation (Guraya, 1975) constituted the control observations.…”

Section: Methodsmentioning

confidence: 99%

“…The CL of untreated rats during the corresponding days of the oestrous cycle and pregnancy showed sparsely scattered lipid droplets which consisted mainly of phospholipids and very few triglycérides (Guraya, 1975).…”

Section: Effects Of Oestradiol Benzoate Alonementioning

confidence: 95%

“…In previous papers (Guraya, 1972(Guraya, , 1975), a detailed histochemical study was made of lipid changes in the CL of intact rats during the oestrous cycle, pseudopregnancy, pregnancy and lactation. In their amount and histochemical composition, the lipid droplets of CL vary greatly during these reproductive 67 68 S. S. Guraya states.…”

Section: Introductionmentioning

confidence: 99%

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Histochemical Observations on the Lipid Changes in Rat Corpora Lutea During Various Reproductive States After Treatment With Exogenous Hormones

Guraya¹

1975

Reproduction

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Lipid changes were studied histochemically in rat CL during the oestrous cycle, pregnancy and lactation after treatment with oestradiol benzoate (EB), LH and prolactin. A single injection of 50 mug EB given on any day during the first half of the oestrous cycle, pregnancy and lactation caused a heavy accumulation of lipid droplets, which consisted mainly of cholesterol and/or its esters, triglycerides and some phospholipids. If the treatment with EB was followed by a single injection of 20 i.u. prolactin, droplet accumulation either did not occur, or was greatly reduced. During the second half of pregnancy, a massive accumulation of cholesterol- and triglyceride-positive lipid droplets occurred in the CL of untreated rats, and prolactin was equally effective in reducing, or preventing the storage of cholesterol-positive lipid droplets. Prolactin was more effective if two injections of 10 i.u. were given daily after an interval of 11 to 12 hr. No appreciable lipid change in the CL was caused by injection of 100 mug LH on any day during the first half of the oestrous cycle and pregnancy, but during lactation the same amount caused a heavy accumulation of lipid droplets, consisting mainly of cholesterol and/or its esters, triglycerides and very little phospholipid. Persisting CL of pregnancy and cyclic CL seen during lactation and pregnancy, respectively, were unresponsive to exogenous hormones, apparently due to ageing as no lipid changes occurred in the CL after hormonal treatment. The results are discussed in relation to previous histochemical and biochemical studies and suggest that both LH and prolactin regulate the lipid and cholesterol metabolism in the luteal cells of the rat.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Effects Of Oestradiol Benzoate Alonementioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Histochemical Observations on the Lipid Changes in Rat Corpora Lutea During Various Reproductive States After Treatment With Exogenous Hormones

Guraya¹

1975

Reproduction

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“…The day spermatozoa were found in the vaginal smear was designated Day 1 of pregnancy, and the day of parturition was designated Day 1 of lactation. Details of the histochemical methods and hormones used have been described in previous papers (Guraya, 1975a, b Pearse, 1968 (Guraya, 1975a (Guraya, 1973). (Zarrow & Clark, 1969;Armstrong et al, 1969;Guraya, 1973), but the exact mechanism of its action is still controversial.…”

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Histochemical Observations on the Lipid Changes in the Ovarian Interstitial Gland Tissue of Rats

Guraya¹

1975

Reproduction

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Summary. Lipid changes were studied histochemically in the ovarian interstitial tissue of untreated and hormone-treated rats during the oestrous cycle, pseudopregnancy, pregnancy and lactation. The hormones tested were LH, prolactin and oestradiol benzoate, alone or in combination. Conspicuous lipid changes occurred only in response to LH.The lipid droplets of CL in normal untreated rats show conspicuous changes in their amount and histochemical composition during various reproductive states, and seem to be regulated by the changing levels of LH and prolactin (Guraya, 1975a, b). In order to determine the comparative response of CL and interstitial gland tissue to endogenous and exogenous hormones, a parallel histochemical study was also carried out on the lipid changes of the latter in intact and hormone-treated rats during different reproductive states, and these results are reported here.The lipids were histochemically analysed in the ovarian interstitial tissue of intact and hormone-treated Holtzman rats during the oestrous cycle, pseudopregnancy, pregnancy and lactation. The rats, weighing 255 to 300 g, were kept in an air-conditioned room with a controlled lighting regimen of 14 hr light/10 hr dark. Vaginal smears were examined each morning and only rats with regular 4-or 5-day oestrous cycles were used. The day spermatozoa were found in the vaginal smear was designated Day 1 of pregnancy, and the day of parturition was designated Day 1 of lactation. Details of the histochemical methods and hormones used have been described in previous papers (Guraya, 1975a, b

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Histochemistry of the Ovary

Guraya¹

1980

Biology of the Ovary

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Histochemical Observations on the Lipid Changes in the Rat Corpus Luteum During Various Reproductive States

Abstract: A histochemical study has been made of lipid changes in rat CL during the oestrous cycle, pseudopregnancy, pregnancy and lactation. The possible physiological significance of these lipid changes in relation to steroid biosynthesis has been discussed.

Cited by 12 publications

References 24 publications

Histochemical Observations on the Lipid Changes in Rat Corpora Lutea During Various Reproductive States After Treatment With Exogenous Hormones

Histochemical Observations on the Lipid Changes in Rat Corpora Lutea During Various Reproductive States After Treatment With Exogenous Hormones

Histochemical Observations on the Lipid Changes in the Ovarian Interstitial Gland Tissue of Rats

Histochemistry of the Ovary

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