Histochemical Methods for Separate, Consecutive and Simultaneous Demonstration of Acetylcholinesterase and Norepinephrine in Cryostat Sections

Elbadawi, Ahmad; Schenk, Eric A.

doi:10.1177/15.10.580

Cited by 389 publications

(69 citation statements)

References 8 publications

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“…The chickens and pheasants were killed by aether narcosis and their bursae cloacales were removed. ACHE-positive nerve components were visualized by the direct tiocholin method cytochemistry evidence acetylcholinesterase by El-Badawi and Schenk (1967), adrenergic nerve components we visualized with glyoxylic acid in modification of Shvalev and Zhuckova (1987). Microscopic examination and photo documentation were performed using the NIKON.…”

Section: Methodsmentioning

confidence: 99%

The Autonomic Innervation of the Bursa Cloacalis of Chickens and Pheasants

Kočišová¹,

Dorko²,

Schmidtová³

et al. 2000

Pakistan J. of Biological Sciences

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Abstract:The aim of the present study was to compare the presence and topography of intra bursal adrenergic and ACHE-positive nerve structures in the bursa cloacalis of the chickens and pheasants from hatching until 12 months of age. The adrenergic nervous profiles were demonstrated visualized by means of verified histochemical method with glyoxylic acid and for demonstration of ACHE-positive nerve fibres were used the direct tiocholin method cytochemistry evidence acetylcholinesterase. In the bursa cloacalis the distribution and topography of adrenergic and ACHE-positive nerve formation in chickens and pheasants were corresponding. The visible nerve fibres were founded mainly in close connection with vessels or as solitary fibres running through fibrous septa around the lymphatic follicles. Nerve fibres in germinal center of lymphatic follicles were not discover. The highest density of adrenergic and ACHE-positive nerves was observed in the period between 3rd and 4th months of age. After this period gradual reduction of adrenergic and ACHE-positive nerve structures was observed and the bursa cloacalis of a 12 months old chicken and pheasant showed only single nerve structures located mainly in the perivascular topography.

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Section: Methodsmentioning

confidence: 99%

The Autonomic Innervation of the Bursa Cloacalis of Chickens and Pheasants

Kočišová¹,

Dorko²,

Schmidtová³

et al. 2000

Pakistan J. of Biological Sciences

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“…• C. The acetylcholinesterase (AChE)-positive fibers in the thymus and bursa of Fabricius were visualized by a direct thiocholine method according to El Badawi & Schenk (1967) to provide cytochemical proof of AChE. In this method acetylcholinesterase present in the cholinergic nerves releases thiocholine from acetylthiocholine, which reduces potassium ferricyanide to potassium ferrocyanide, capable of producing insoluble copper ferrocyanide with copper ions (Hatchett's brown).…”

Section: Methodsmentioning

confidence: 99%

Cholinesterase activity in quail primary lymphoid organs

et al. 2013

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The present histochemical study was carried out to analyze the distribution and topography of acetylcholinepositive nerve fibers in the thymus and bursa of Fabricius of quails. The AChE-positive nerve fibers were demonstrated by direct thiocholine histochemical method. Nerve fibers present in the thymuses form periarterial nerve plexuses located mostly in the interlobular septa and on the cortico-medullary junction. Vessels-independent nerve fibers occur also in the parenchyma of thymic medulla, but rarely in parenchyma of the cortex. Within the connective tissue between the bursa of Fabricius and the wall of proctodeum we observed conspicuous AChE-positive ganglia, often in close relationship to greater arteries. Within the wall of bursa of Fabricius, AChE-positive nerve fibers create nerve plexuses around arteries. We observed a close relationship between lymphoid follicules in bursal submucosa and mucosa and AChE-positive nerve fibers. Nerve fibers create a ring around lymphoid follicles, but do not penetrate into the germinal center of the follicle. Arteries inside quail thymuses and bursae of Fabricius contain rich AChE-positive nerve plexuses, when compared to the veins, which have a very poor presence of AChE-positive nerves. According to lesser presence and decreased density of AChE-positive nerve fibers in older animals, we described age-dependent changes in both quail primary lymphoid organs.

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“…The specific fluorescence of adrenergic nerve fibres was studied in incident ultraviolet light (epiluminiscence) using a microscope Jenalumar 2 (Zeiss, Jena). The presence of acetylcholinesterase in splenic neural structures was determined by the direct thiocholine method modified by El-Badawi and Schenk (1967). After incubation, the individual sections were mounted on slides and examined under a light microscope Jenalumar 2.…”

Section: Methodsmentioning

confidence: 99%

Adrenergic and Cholinergic Innervation of the Pheasant Spleen

Schmidtová¹,

Kočišová²,

Dorko³

et al. 2000

Pakistan J. of Biological Sciences

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Abstract:The localization and distribution of the adrenergic and acetylcholinesterase (AChE)-positive nerve fibres innervating the spleen was studied in pheasant by using histochemical methods. Both visualized nerve fibres entered the pheasant spleen at its hilum in the vicinity of the splenic artery branches and they were gradually distributed into the parenchyma along large arterial branches. The greatest number and density of adrenergic and cholinergic nerve fibres were associated with the central artery of the white pulp, and from this localization entered to the periarterial lymphatic sheath. Some of these nerve profiles extended to the red pulp and reached the splenic capsule. The adrenergic nerve fibres were found in the well of large and small branches of the splenic veins, but no cholinergic nerve fibres were found there. The abundant occurence of adrenergic and cholinergic nerve fibres in several parts of the pheasant spleen suggest about their participation on modulation of splenic functions.

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Histochemical Methods for Separate, Consecutive and Simultaneous Demonstration of Acetylcholinesterase and Norepinephrine in Cryostat Sections

Cited by 389 publications

References 8 publications

The Autonomic Innervation of the Bursa Cloacalis of Chickens and Pheasants

The Autonomic Innervation of the Bursa Cloacalis of Chickens and Pheasants

Cholinesterase activity in quail primary lymphoid organs

Adrenergic and Cholinergic Innervation of the Pheasant Spleen

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