Histochemical localization of ?5-3 ?-hydroxysteroid dehydrogenase, glucose-6-phosphate dehydrogenase, and NADH- and NADPH-diaphorase activities in the testis ofRana hexadactyla andCacopus systoma

Saidapur, Srinivas K.; Nadkarni, V. B.

doi:10.1007/bf01003793

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…The greater activity of steroid dehydrogenases in seminiferous tubules of goats, in contrast to other mammalian species (Blackshaw, 1970;Saidapur, 1976;Ewing & Brown, 1977), suggested that seminiferous tubules, as well as interstitial tissue, may produce some testosterone. A similar suggestion has been made for the ram testis (Baillie et al 19666;Voglmayr et al 1966) in which no significant amount of 3ß-HSD could be demonstrated in interstitial tissue (Hay & Deane, 1966;Samisoni, 1966).…”

Section: Discussionmentioning

confidence: 88%

Histochemical studies on steroid dehydrogenases in the testis of the goat (Capra hircus)

Bilaspuri¹,

Guraya²

1984

Journal of Endocrinology

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Detailed histochemical localization of 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and 17 beta-HSD was made in the goat testis using both NAD and NADP coenzymes. The substrates used for 3 beta-HSD were dehydroepiandrosterone (DHA) and pregnenolone whereas 17 beta-HSD was localized with testosterone and oestradiol. In general, the activity of the enzymes varied with the cell type, substrate and coenzyme. In seminiferous tubules, DHA and NAD were the preferred substrate and coenzyme respectively for 3 beta-HSD. In addition, in interstitial tissue, NAD was the preferred coenzyme with DHA whereas no such preference existed with pregnenolone. 17 beta-Hydroxysteroid dehydrogenase showed a similar pattern in the two main compartments of the testis, as testosterone and oestradiol were equally utilized and NAD was the preferred coenzyme in both these compartments. The activities of the enzymes increased during the process of spermiogenesis and were higher in seminiferous tubules than in interstitial tissue, especially in elongated spermatids and spermatozoa.

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Section: Discussionmentioning

confidence: 88%

Histochemical studies on steroid dehydrogenases in the testis of the goat (Capra hircus)

Bilaspuri¹,

Guraya²

1984

Journal of Endocrinology

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“…Histological examination by conventional methods has generally been the only assessment of annual variations in Leydig cells for the majority of bats studied (see Table 2 Christensen & Gillim, 1969;Christensen, 1975;Neaves, 1975Neaves, , 1977 (Vignoli, 1930;Maeir, 1965;Racey & Tarn, 1974;Gustafson, 1975aGustafson, , b, 1976Saidapur, 1976 (Gustafson, 1975a(Gustafson, , b, 1976 (Gustafson, 1975a(Gustafson, , 1976 Johnson, 1970). This cycle is also consistent with current cytophysiological concepts of Leydig cell function.…”

Section: Patterns Of Male Cyclesmentioning

confidence: 99%

Male reproductive patterns in hibernating bats

Gustafson¹

1979

Reproduction

130

100

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“…In other species, it may be present in both the testicular compartments or it may be primarily an interstitial enzyme (Blackshaw, 1970;Livni & Yaffe, 1974;Saidapur, 1976). The greater activity of G-6-PDH in spermatogonia suggests that these cells are the active sites of the pentose cycle for locally supplying ribose in these actively dividing cells.…”

Section: Discussionmentioning

confidence: 96%

“…Glucose-6-phosphate dehydrogenase (G-6-PDH) is an important enzyme of the pentose cycle and its distribution has been studied in the testes of several species (Blackahaw, 1970;Livni & Yaffe, 1974;Saidapur, 1976). Some aspects of testes of buffaloes and goats have been investigated in our laboratory (Guraya & Bilaspuri, 1976;Bilaspuri, 1978;Bilaspuri & Guraya, 1978, 1980a, 6, 1981, 1982, 1983a.…”

mentioning

confidence: 98%

Histochemical localization of enzymes of various metabolic pathways in the testes of buffaloes, goats and rams

Bilaspuri

Guraya

1984

J. Agric. Sci.

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Isocitrate dehydrogenase (ICDH), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), glutamate dehydrogenase (GDH), /Miydroxybutyrate dehydrogenase (/?-0H-BDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) were histochemically located in the testes of buffaloes, goats and rams. The enzyme activities varied with the enzyme, species and cell type. The activities in the seminiferous tubules were correlated with the stages of seminiferous epithelial cycle (SEC). During this cycle, the activities in the Sertoli cells, spermatogonia and spermatocytes remained unaltered in contrast to those in the spermatids. The activities of SDH, ICDH and MDH were relatively greater in buffalo, while goat and ram resembled each other quite closely. ICDH and MDH preferred NADP to NAD. In the three species, the activities of ICDH, SDH and MDH generally followed an increasing order. G-6-PDH was greater in the interstitial tissue of buffalo than in goat and ram; the maximum activity of this enzyme in each species was found in the spermatogonia. In comparison with G-6-PDH, GDH was less evident in the interstitial tissue of buffalo and goat; Sertoli cells and spermatogonia also showed relatively less MDH activity whereas the other germ cells may have relatively less, similar or more, GDH activity depending on the species. /?-OH-BDH activity was similar in the interstitial tissue of the three species, but in the seminiferous tubule, the activity was less in goat. But for GDH and /?-OH-BDH which could show different results, the activities of other enzymes generally decreased from spermatogonia through spermatocytes to spermatids but increased during spermiogenesis. In spermatozoa, the enzymes were observed only in the mid-piece. The possible physiological significance of the results is discussed in relation to different metabolic pathways.

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Histochemical localization of ?5-3 ?-hydroxysteroid dehydrogenase, glucose-6-phosphate dehydrogenase, and NADH- and NADPH-diaphorase activities in the testis ofRana hexadactyla andCacopus systoma

Cited by 6 publications

References 13 publications

Histochemical studies on steroid dehydrogenases in the testis of the goat (Capra hircus)

Histochemical studies on steroid dehydrogenases in the testis of the goat (Capra hircus)

Male reproductive patterns in hibernating bats

Histochemical localization of enzymes of various metabolic pathways in the testes of buffaloes, goats and rams

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