Histochemical investigations on the nature of large blood vessel endothelial and medial argyrophilic lines and on the mechanism of silver staining

Light microscopy, scanning electron microscopy, and transmission electron microscopy were employed on the anterior wall of the rabbit thoracic aorta with the particular purpose to study endothelial marginal flaps and the structure of stomata under physiological conditions at 100 mm Hg. Intra-aortic pressure monitoring was performed during the whole period of perfusion (2 h), restricting pressure variations to a maximum of ± 5 mm Hg in order to preserve endothelial architecture. We found the endothelial surface uniformly smooth. Marginal flaps of the endothelial cells were mutually overlapping, often forming multiple layers. Underlying flaps might protrude to the lumen between neighbouring flaps as flat mushrooms. Flaps of adjacent cells might diverge for short distances to decover an underlying endothelial cell. Downstream flaps of the cells might be overlapped at their base by flaps of adjacent cells, the extremity of the flap thus appearing as a disc separated from the cell. The small discs and troughs thus formed are considered equivalent to the so-called stomata.

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“…Similar experiences have previously been reported on formaldehyde-fixed mate rial for surface light microscopy [Gottlob and Hoff, 1968].…”

Section: Discussionsupporting

confidence: 68%

Scanning and Transmission Electron Microscopy of the Normal Rabbit Aortic Endothelium after Controlled Perfusion Fixation

Garbarsch¹,

Tranum‐Jensen²,

Deurs³

1982

Cells Tissues Organs

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“…Under electron microscopic observation, both types of fibers show the same periodicity (Wasserman and Kubota 1956;Irving and Tomlin 1954). Some workers showed that their different coloration is due to the differences which exist between the ground substance surrounding both types of fibers (Gottlob and Hoff 1968;Valdrighi and Vidal 1971). As mentioned above, acid mucopolysaccharides were increased in cervix during pregnancy and they differed from those in nonpregnant cervix.…”

Section: Discussionmentioning

confidence: 98%

Histological Aspects of Cervical Ripening

Ichijo¹,

Shimizu²,

Sasai

1976

Tohoku J. Exp. Med.

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“…To visualize mesothelial cells by light microscopy, the mesothelium was processed for silver staining as previously described (Gottlob and Hoff, 1968). A three grades injury score (IS) was used to classify the specimen as normal, injured, or heavily injured, according to the extent of the surface of the specimen covered by normal mesothelial cells (>95%, IS=0; 95-50%, IS=1; <50%, IS=2).…”

Section: Histologymentioning

confidence: 99%