Histochemical investigations of Rotifera Bdelloidea. I. Localization of cholinesterase activity

Raineri, Margherita

doi:10.1007/bf01003388

Cited by 24 publications

(18 citation statements)

References 38 publications

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“…These areas stained for the hormones match the areas where the colinergic and catecolaminergic systems have been reported (Nogrady & Alai 1983, Raineri 1984, Kotikova, 1995, 1998, Kotikova et al 2005, Pineda-Rosas et al 2005. Furthermore, in these areas there have been already reports of the presence of exocitotic proteins in rotifers, suggesting the presence of a cholinergic system coupled to a hormone release system (Pérez-Legaspi et al 2008).…”

Section: Discussionsupporting

confidence: 78%

“…The catecholaminergic and cholinergic system of several rotifer species has been studied by several authors (Nogrady & Alai 1983, Raineri 1984, Kotikova 1995, 1998, Kotikova et al 2005, Pineda-Rosas et al 2005, who have identified acethylcholinesterase activity and its receptors were described and the general characteristics of these systems were corroborated. The cholinergic system is involved in the reproduction and the fertility control because some neuropeptides control the synthesis of selected glycoproteins like the luteinizing hormone (LH) and gonadotropin (Vijayan 1985).…”

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confidence: 99%

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Immunodetection of Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in Brachionus calyciflorus (Rotifera: Monogononta)

Alvarado-Flores¹,

Montoya-Garcia²,

Ventura-Juárez³

et al. 2008

RBT

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Abstract:The endocrine system controls and coordinates behavioral, biochemical, and physiological processes through signal mechanisms using neuropeptides or products of neurosecretory cells. Among invertebrates, this system is poorly studied in rotifers, in which estrogens and androgens significantly affect sexual reproduction. This is the first report of the presence of the Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in rotifers. Analyses included the avidin-biotin-peroxidase complex method with primary antibodies LH (Anti-Rat LH serum for RiA), PRL (Anti-Rat PRL serum for RiA), FSH (Anti-Rat FSH serum for RiA) and TSH (Anti-Rat TSH serum for RiA). These hormones were found in females, males and parthenogenetic and sexual eggs of the freshwater Brachionus calyciflorus. The immunoreactivity of FSH, LH, TSH and PRL in females was observed in: ovaries, cerebrum, mastax, stomach, lorica, and the stomach gland. However, in males LH was observed only at the trochal disk and cerebrum. The hormones FSH, TSH and PRL, were observed in testicles, contractil vesicles, and cementary gland of males. Regarding amictic or parthenogenetic eggs, the hormones LH, FSH, TSH, and PRL were located mainly in the micromeres, and the staining in the macromeres was weak. On the other hand, in the mictic or sexual eggs the inner shell is stained for the hormones PRL and LH, opposite to the staining of FSH and TSH, located mainly in the embryo. in general, immuno-reactivity was observed in areas important for the reproductive, excretory, digestive and developmental processes. Rev. Biol. Trop. 57 (4):

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Section: Discussionsupporting

confidence: 78%

mentioning

confidence: 99%

Immunodetection of Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in Brachionus calyciflorus (Rotifera: Monogononta)

Alvarado-Flores¹,

Montoya-Garcia²,

Ventura-Juárez³

et al. 2008

RBT

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“…In the brain of Bdelloida two types of cholinergic (Ch-ergic) neurons were described (Raineri, 1984) . A series of ultrastructural investigations permitted to reveal at least 4 types of synaptic vesicles in the neurons of rotifers (for reviews see Clement, 1977 ;Wurdak et al, 1983) .…”

Section: Introductionmentioning

confidence: 99%

Localization and neuroanatomy of catecholaminergic neurons in some rotifer species

Kotikova

1995

Hydrobiologia

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We studied Dicranophorus sp ., Platyias quadricornis (Ehrb .) and Rotaria tardigrada (Ehrb .) . These rotifers, systematically distant from each other, show the same pattern of the catecholaminergic (CA-ergic) part of the nervous system . It is formed of a small (23-24), but steady number of neurons characteristic for each species . Three types of CA-ergic neurons are described. The sizes of neurons vary from to two to ten µm . The distribution of the brain neurons is correlated with body shape . Such a type of nervous system is topographically comparable to the concentrated orthogon of the flatworms . 123

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“…The same effect can be observed in the rotifers Brachionus calyciflorus, B. angularis, and B. patulus exposed to methyl-parathion (Fernández-Casalderrey et al, 1993;Flores et al, 1999;Sarma et al, 2001;Flores et al, 2004) and the rotifer Lecane quadridentata exposed to carbaryl and methyl-parathion (Pérez-Legaspi et al, 2012). Thus, since rotifers are aquatic invertebrates with cholinergic (Nogrady and Alai, 1983;Raineri, 1984;Pineda-Rosas et al, 2005), catecholaminergic (Kotikova 1995), and serotoninergic (Kotikova et al, 2005) nervous system, there are susceptible to toxic effects of these pesticides in the aquatic environment. The impact of pollutants on the distribution of rotifer species, can be decisive in aquatic ecosystems (Medeiros et al, 2010).…”

Section: Introductionmentioning

confidence: 58%

Reduced expression of exocytotic proteins caused by anti-cholinesterase pesticides in Brachionus calyciflorus (Rotifera: Monogononta)

2015

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The organophosphate and carbamate pesticides methyl-parathion and carbaryl have a common action mechanism: they inhibit acetylcholinesterase enzyme by blocking the transmission of nerve impulses. However, they can alter the expression of exocytotic membrane proteins (SNARE), by modifying release of neurotransmitters and other substances. This study evaluated the adverse effects of the pesticides methyl-parathion and carbaryl on expression of SNARE proteins: Syntaxin-1, Syntaxin-4 and SNAP-23 in freshwater rotifer Brachionus calyciflorus. Protein expression of these three proteins was analyzed before and after exposure to these two pesticides by Western Blot. The expression of Syntaxin-1, Syntaxin-4 and SNAP-23 proteins in B. calyciflorus significantly decreases with increasing concentration of either pesticides. This suggests that organophosphates and carbamates have adverse effects on expression of membrane proteins of exocytosis by altering the recognition, docking and fusion of presynaptic and vesicular membranes involved in exocytosis of neurotransmitters. Our results demonstrate that the neurotoxic effect of anticholinesterase pesticides influences the interaction of syntaxins and SNAP-25 and the proper assembly of the SNARE complex.Keywords: SNARE, pesticide, anticholinesterase, neuroexocytosis, rotifer. Redução da expressão de proteínas de exocitose causados por pesticidas anti-colinesterase em Brachionus calyciflorus (Rotifera: Monogononta) ResumoOs pesticidas organofosforados e carbamatos metil-paration e carbaril tem um mecanismo de ação comum: eles inibem a enzima acetilcolinesterase, bloqueando a transmissão dos impulsos nervosos. No entanto, eles podem alterar a expressão de proteínas de membrana de exocitose (SNARE), através da modificação da libertação de neurotransmissores e outras substâncias. Este estudo avaliou os efeitos adversos dos pesticidas metil-paration e carbaril sobre a expressão de proteínas SNARE: Sintaxina -1, Sintaxina-4 e SNAP-23 em rotíferos de água doce Brachionus calyciflorus. A expressão destas três proteínas foi analisada antes e depois da exposição a estes dois pesticidas por Western Blot. A expressão das proteínas Sintaxina-1, Sintaxina-4 e SNAP-23 em B. calyciflorus diminui significativamente com o aumento da concentração de ambos os pesticidas. Isto sugere que os organofosfatos e carbamatos têm efeitos adversos sobre a expressão de proteínas de membrana de exocitose, alterando o reconhecimento, de encaixe e fusão de membranas pré-sinápticas e vesiculares envolvidas na exocitose de neurotransmissores. Nossos resultados demonstram que o efeito neurotóxico de pesticidas anticolinesterásicos influencia a interação de sintaxinas e SNAP-25 e a montagem correta do complexo SNARE.

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Histochemical investigations of Rotifera Bdelloidea. I. Localization of cholinesterase activity

Cited by 24 publications

References 38 publications

Immunodetection of Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in Brachionus calyciflorus (Rotifera: Monogononta)

Immunodetection of Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in Brachionus calyciflorus (Rotifera: Monogononta)

Localization and neuroanatomy of catecholaminergic neurons in some rotifer species

Reduced expression of exocytotic proteins caused by anti-cholinesterase pesticides in Brachionus calyciflorus (Rotifera: Monogononta)

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