Histochemical, immunohistochemical, and ultrastructural studies of gingival fibromatosis: a case report

Abstract

Gingival fibromatosis is a rare disease characterized by enlargement of the gingiva. The purpose of this study was to analyze a case of idiopathic gingival fibromatosis, using histochemical and immunohistochemical staining and transmission electron microscopy. The patient was a 39-year-old Japanese man, in whom the gingiva was enlarged throughout the entire mandible and maxilla. Specimens of gingival fibromatosis exhibited epithelial hyperplasia and increased amounts of collagen fiber bundles in the connective tissue light-microscopically. Well-developed collagen bundles were strongly stained with Azan and Masson trichrome staining. Immunohistochemically, the gingival connective tissue was specifically stained by type I collagen and vimentin antibodies. Ultrastructurally, the lesion consisted of fibroblasts and mature collagen fibers running in all directions. No myofibroblasts were detected histochemically, immunohistochemically, or ultrastructurally. These findings suggested that this disease may be the result of an increase in collagen synthesis by the fibroblasts and/or that it may be associated with one of the findings of histologic heterogeneity.

Proliferation of Pulmonary Interstitial Fibroblasts Is Mediated by Transforming Growth Factor-β1-induced Release of Extracellular Fibroblast Growth Factor-2 and Phosphorylation of p38 MAPK and JNK

“…1D). Vimentin stains mesenchymal cells (23). Alveolar macrophages, which are of hematopoietic lineage, were used as a negative control for vimentin and did not stain with vimentin (Fig.…”

“…Prior to reprobing, the same nitrocellulose membrane was incubated at 50°C for 30 min with stripping buffer (100 mM 2-mercaptoethanol, 2% SDS, and 62.5 mM Tris-HCl (pH 6.7)) (10). After a rinse in TBS, the membranes were incubated with the ECL detection reagents and exposed to Kodak film (23,28,29). If no band was detected, the same nitrocellulose membrane was blocked using 5% instant skim milk in TBS, followed by incubation with the primary and secondary antibodies as described (10,15,16).…”

“…The L-s223,225-TGF-␤1 cDNA contains a mutation in the TGF-␤1 gene in which Cys 223 and Cys 225 have been substituted with serines (10). This mutation results in constitutive release of biologically active TGF-␤1 (23). Retroviruses such as pMX enter only actively proliferating cells (10).…”

Genetic Disorders Associated with Gingival Enlargement

“…The other contains prominent cytoplasm with well developed organelles. Those fibroblasts have been considered inactive and active, respectively (Collan et al, 1982;Sakamoto et al, 2002). The connective tissue in HGF also exhibits an accumulation of elastic and oxytalan fibers (Baptista, 2002;Chavrier & Couble, 1979;Doufexi et al, 2005;Hart et al, 2000;Sakamoto et al, 2002).…”

“…Those fibroblasts have been considered inactive and active, respectively (Collan et al, 1982;Sakamoto et al, 2002). The connective tissue in HGF also exhibits an accumulation of elastic and oxytalan fibers (Baptista, 2002;Chavrier & Couble, 1979;Doufexi et al, 2005;Hart et al, 2000;Sakamoto et al, 2002). Although a rare finding, small osseous calcifications and abundant neurovascular bundles may also be present (Gunhan et al, 1995;Kelekis-Cholakis et al, 2002).…”