Histochemical features of neurons in the cat stellate ganglion during postnatal ontogenesis

Maslyukov, P. M.; Shilkin, V. V.; Nozdrachev, A. D.; Timmermans, Jean‐Pierre

doi:10.1016/s1566-0702(03)00051-1

Cited by 9 publications

(10 citation statements)

References 32 publications

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“…It means that these cell bodies are noradrenergic. This is in agreement with previously obtained data on the paravartebral ganglia of the rat neonates and pups [15], newborn and young mice [13,18], newborn and young mice and rats [21], and on the prevertebral ganglia of the pig [17]. The remaining small population of neurons in the greater splanchnic nerve did not express TH or DBH.…”

Section: Discussionsupporting

confidence: 93%

“…There was an increase in the proportion of NPY-positive neurons during postnatal period from birth to 60 dpp of rats and mice [13,15,18,21]. Thus, our findings are in accord with the results of previous studies describing the population of NPY-positive neurons in the postnatal period in rat and mouse [13,15,18,21], dog and cat [22], and pig [17].…”

Section: Discussionsupporting

confidence: 92%

“…NPY was widely distributed in neurons throughout the porcine greater splanchnic nerve ganglia. NPY is a major co-transmitter in the sympathetic ganglia, which is in accordance with immunohistochemistry reports in neurons of the sympathetic ganglia in pig [11], newborn and young mice [13], sheep [14], rat neonates and pups [15], and humans [16]. Previous studies described various size of the population of NPY-IR neurons in sympathetic ganglia in different mammals: 80% in the porcine celiac-superior mesenteric ganglia (CSMG) [17], 80-85% in the superior cervical ganglion (SCG) and middle cervical ganglion (MCG) of the cats and dogs [22], 40-60% in porcine stellate ganglion (SG) [10], 5-10% in porcine thoracolumbar ganglia [11].…”

Section: Discussionsupporting

confidence: 89%

“…Previous studies described various size of the population of NPY-IR neurons in sympathetic ganglia in different mammals: 80% in the porcine celiac-superior mesenteric ganglia (CSMG) [17], 80-85% in the superior cervical ganglion (SCG) and middle cervical ganglion (MCG) of the cats and dogs [22], 40-60% in porcine stellate ganglion (SG) [10], 5-10% in porcine thoracolumbar ganglia [11]. There was an increase in the proportion of NPY-positive neurons during postnatal period from birth to 60 dpp of rats and mice [13,15,18,21]. Thus, our findings are in accord with the results of previous studies describing the population of NPY-positive neurons in the postnatal period in rat and mouse [13,15,18,21], dog and cat [22], and pig [17].…”

Section: Discussionmentioning

confidence: 99%

“…The role of neuropeptides as putative neurotransmitters has become an important consideration in the study of nervous system functions. Different neuropeptides have previously been reported to exist in neurons of paravertebral and prevertebral ganglia in various mammalian species, including vasoactive intestinal polypeptide (VIP) in the pig [10,11], cat [12], newborn and young mice [13], sheep [14], rat neonates and pups [15], and humans [16]; encephalin (MEAGL) in the pig [10,11]; neuropeptide Y (NPY) in the pig [10,11], cat [12], newborn and young mice [13], sheep [14], rat neonates and pups [15], and humans [16]; substance P (SP) in the pig [10,11], cat [12], sheep [14], and humans [16]; calcitonin gene-related peptide (CGRP) in the pig [10,11], cat [12], newborn and young mice [13], sheep [14], and rat neonates and pups [15]; galanin (GAL) in the newborn and very young mice [13], sheep [14], rat neonates and pups [15], and pig [17]; somatostatin (SOM) in the pig [10,11], cat [12], rat neonates and pups [15], humans [16], and newborn and young mice [18]. In this study, attention has been focused on the neurochemical characteristics of neurons in the porcine greater splanchnic nerve, because until now the porcine GSN neurons have yet not been characterized by immunohistochemistry.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Nourinezhad

Podlasz

Wąsowicz

2018

Folia Histochem Cytobiol.

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Introduction. Greater splanchnic nerve (GSN) is by far the largest of the splanchnic nerves and connects the paravertebral and prevertebral ganglia to transmit the majority of nociceptive information from the viscera. Despite its importance, the immunohistochemical features of the porcine GSN neurons have not yet been examined. Therefore, the aim of the study was to investigate the neurochemistry of the porcine GSN neurons and to compare their neurochemical coding with those of the paravertebral and prevertebral ganglia. Material and methods. Four gilts of Large White Polish breed were examined in this study. Antibodies to tyrosine hydroxylase (TH), dopamine b-hydroxylase (DBH), choline acetyltransferase (ChAT), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), somatostatin (SOM), galanin (GAL), methionine-enkephalin (MET), calcitonin gene-related peptide (CGRP), and substance P (SP) were used for immunohistochemical detection of classical neurotransmitters marker enzymes and neuropeptides in neuronal cell bodies of the GSN. Results. Double-labeling immunofluorescence revealed that virtually all GSN neurons exhibited the presence of catecholamine-synthesizing enzymes (TH/DBH-positive) and subpopulations of neurons contained immunoreactivity to NPY, VIP, SOM, GAL and MET. However, CGRP and SP-immunoreactivity were not observed in neuronal somata. Conclusions. Our data strongly suggest that the general immunohistochemical characterization of ganglion cells in the porcine greater splanchnic nerve is similar to that of the prevertebral ganglia (e.g. celiacomesenteric ganglion).

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Nourinezhad

Podlasz

Wąsowicz

2018

Folia Histochem Cytobiol.

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Immunocytochemical properties of stellate ganglion neurons during early postnatal development

Maslyukov

Timmermans

2004

Histochem Cell Biol

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Neurotransmitter features in sympathetic neurons are subject to change during development. To better understand the neuroplasticity of sympathetic neurons during early postnatal ontogenesis, this study was set up to immunocytochemically investigate the development of the catecholaminergic, cholinergic, and peptidergic phenotypes in the stellate ganglion of mice and rats. The present study was performed on Wistar rats and Swiss mice of different ages (newborn, 10-day-old, 20-day-old, 30-day-old, and 60-day-old). To this end, double labeling for tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), vasoactive intestinal (poly)peptide (VIP), neuropeptide Y (NPY), galanin (GAL), and somatostatin (SOM) was applied. The results obtained indicate that the majority of the neurons in the stellate ganglion of both species were TH-positive from birth onward and that a large part of these neurons also contained NPY. The percentage of neurons containing TH and NPY invariably increased with age up to 60 days postnatally. A smaller portion of the stellate ganglion neurons contained other types of neuropeptides and showed a distinct chronological pattern. The proportion of VIP- and ChAT-positive neurons was maximal in 10-day-old animals and then decreased up to 60 days of age, whereas the number of SOM-positive cells in rats significantly decreased from birth onward. In newborn rats, VIP-, ChAT- and SOM-positive neurons were largely TH-positive, while their proportions decreased in 10-day-old and older rats. Accordingly, the largest part of VIP-positive neurons also expressed SOM immunoreactivity at birth, after which the number of neurons containing both peptides diminished. The VIP- and SOM-positive cells did not contain NPY in any of the age groups studied. In rats up to 10 days of life, GAL-immunoreactive (-IR) neurons were scarce, after which their number increased to reach a maximal value in 30-day-old animals and then declined again. The SOM-reactive cells had the smallest size in all rats, while the largest neurons were those containing ChAT. In the mouse stellate ganglion, VIP- and ChAT-IR neurons were larger in comparison to NPY- and TH-IR cells. Our study further revealed some species differences: compared to mice the proportion of neurons containing TH and NPY was higher in rats at all ages under study. Furthermore, no GAL-immunostained neurons were found in mice and the number of SOM-positive cells in mice was limited compared to that observed in rats. In conclusion, the development of neurotransmitter composition is complete in rats and mice by their second month of life. At this age, the percentages of immunopositive cells have become similar to those reported in adult animals.

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Immunocytochemical characteristics of neurons in the stellate ganglion of the sympathetic trunk in mice during postnatal ontogenesis

Maslyukov

Shilkin

Timmermans

2006

Neurosci Behav Physiol

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The neurotransmitter composition of neurons in the stellate ganglion of mice of different ages (neonatal and aged 10, 20, 30, and 60 days) was studied using an immunocytochemical method. Most of the neurons in the mice of these age groups contained tyrosine hydroxylase (TH). Most choline acetyltransferase (CAT)-positive neurons in neonatal and 10-day mice were also TH-positive. From birth and at all subsequent ages, there were increases in the proportion of cells contained TH and neuropeptide Y. The proportion of neurons containing vasoactive intestinal peptide and CAT increased to 10 days of life and then decreased. Somatostatin-and galanin-positive neurons were absent from all animals. Maturation of the set of neurotransmitters in stellate ganglia was complete by age two months.

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Histochemical features of neurons in the cat stellate ganglion during postnatal ontogenesis

Cited by 9 publications

References 32 publications

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Immunocytochemical properties of stellate ganglion neurons during early postnatal development

Immunocytochemical characteristics of neurons in the stellate ganglion of the sympathetic trunk in mice during postnatal ontogenesis

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