Histochemical distribution of guanase in human tissues with guanine in bicine buffer as substrate.

Ito, Susumu; Hayashi, Kazushi; Iwasaki, Akiharu; Syundo, Jyoji; Ishihara, Akihiko; Tamura, Yoshiyuki; Sendo, Toshiaki; Kunio,

doi:10.1267/ahc.19.437

Cited by 6 publications

(2 citation statements)

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“…We established recently a new method for histochemical demonstration of guanase with nitro blue tetrazolium (NBT) which is useful in assessing the seventies of hepatocellular diseases (7), and studied the distribution of guanase in human tissues (8,9). The histochemical specification of guanase on hepatic disease has also been reported (8,9).…”

Section: Humanmentioning

confidence: 99%

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Histochemical and biochemical studies on guanase in the human and rat kidney.

Ito

Maeda

Iwasaki

et al. 1991

Acta Histochem. Cytochem

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A specific antibody against purified human liver guanase was raised in rabbits. The antiserum formed a single precipitin line with human liver extracts, and completely inhibited the activity of the enzyme derived from human liver and kidney. Immunoblotting showed that the antibody bound only to the one protein band of liver and kidney guanase activity. In immunohistochemical studies, the DAB (3, 3'-diaminobenzidine tetrahydrochloride)-reaction with this antibody was positive in the same locations as the histochemical guanase reaction. Moreover, biochemical data of enzymic activity in nephron segments of the rat indicated that guanase activity was located in the proximal tubules. Thus, the DAB reaction appeared to show the location of guanase itself. This immunohistochemical procedure for detection of guanase should useful in clinical studies on this enzyme.

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Section: Humanmentioning

confidence: 99%

“…The histochemical specification of guanase on hepatic disease has also been reported (8,9). Histochemical and biochemical studies on the location of guanase in the kidney have not been reported.…”

Section: Humanmentioning

confidence: 99%