Histochemical distribution of digestive enzymes in intestine of goldline, Sarpa salpa L. 1758

Abstract

Histochemical localization of non-specific esterase, alkaline and acid phosphatase in the intestine of free-living goldline (Sarpa salpa L. 1758) was investigated. Fish were caught in the vicinity of the town of Zadar (Adriatic Sea, Croatia), and samples of three parts of the intestine proper (anterior, middle and posterior) as well as the rectum were used for presentation of non-specific esterases, alkaline phosphatase and acid phosphatase. Non-specific esterase activity was found in the cytoplasm and brush border of enterocytes in all investigated intestinal segments and the rectum. The activity was stronger in the middle and posterior part of the intestine but weaker in the anterior segment of the intestine as well as in the rectum. Intestinal alkaline phosphatase was detected in the brush border and supranuclear cytoplasm of enterocytes of all investigated intestinal segments. Enzymatic activity gradually decreased in a posterior direction. Acid phosphatase activity was observed as a fine granular reaction product in the supranuclear region of enterocytes and was almost equal in all investigated intestinal segments as well as in the rectum. The possible role of enzymes in intracellular digestion and transport is discussed.

Comparative study on the intestine of Schilbe mystus and Labeo niloticus in correlation with their feeding habits

“…These observations suggest that the enterocytes of the anterior portion of the intestine are involved in lipid absorption. This suggestion is in accordance with that of Stroband and Debets (1978), in Ctenopharyngodon idella, Anderson (1986), in Girella tricuspidata, Deplano et al (1989), in Dicentrarchus labrax, Olsen et al (1999), in Salvelinus alpinus, Gallagher et al (2001), in Lagodon rhomboids, Caballero et al (2003), in Sparus aurata, Kozarić et al (2004), in Merluccius merluccius, and Carrassón et al (2006), in Dentex dentex. Moreover, Fontagné et al (1998), in Cyprinus carpio, stated that the intestinal fat droplets can be interpreted as a temporary storage form of the re-esterified fatty acids.…”

Morphological and histochemical study of intestine in wild and reared European eel (Anguilla anguilla L.)

“…Diverse enzymes involved in digestive and absorptive processes have been reported in a variety of adult fish (Kuz'mina and Gelman 1997;Cahu et al 2000;Kozarić et al 2004;Kozarić et al 2006). Distribution intensity of digestive enzymes across the gastrointestinal tract varies with feeding habits and intestinal morphology (Kolkovski 2001;Gawlicka et al 2002).…”

Histological features of the digestive tract of the adult European hakeMerluccius merluccius(Pisces: Merlucciidae)

“…The two-layered muscularis externa of the European hake oesophagus is made of striated muscular tissue. The activity of enzymes such as non-specific esterase, acid and alkaline phosphatase, was found to be weak to moderate in the hake's oesophagus (Kozarić et al 2004).…”

“…When treated with Alcian blue/PAS, it seems that the intestinal goblet cells contain acidic mucins. Kozarić et al (2004) described that in the European hake intestinal intraepithelial goblet cells showed no enzymatic activity, but dispersed granular enzymatic activity was observed in the connective tissue of lamina propria. The results reported by Ferrando et al (2006) showed that the morphology of the mucosal folds of the Liza aurata intestine, as well as the quality and quantity of the mucous from the intestinal goblet cells, bear a relationship with environmental pollution.…”

“…The mucosal folds in the anterior intestines are more leaf-like, while those in the posterior intestines are finger-like. Even the investigation of enzymatic activity in hake intestines has shown that enzyme activity in anterior intestines differs from those in posterior intestines (Kozarić et al 2004). Goblet cells were found in both parts of the intestines.…”