Histochemical detection of glycoproteins in the gastric epithelia of Columba livia

Lima, Maria Aparecida; Sasso, Wilson da Silva

doi:10.1016/s0065-1281(85)80051-9

Cited by 7 publications

(1 citation statement)

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“…MUC1, MUC5AC and MUC6 are the main constituents of the mucus barrier in the stomach (Audie et al 1993;De Boló s et al 1995;Ho et al 1995a;Jia et al 2010;Reis et al 1997). Previous histochemical studies on the stomach in birds have investigated the localization of the mucin carbohydrates, which are chains of numerous oligosaccharides bound to the mucin core proteins (Mogil'naia & Bogatyr', 1977, 1983Altamirano et al 1984;Inforzato de Lima & Sasso Wda, 1985;Pastor et al 1988;Pinheiro et al 1989;Imai et al 1991;Gheri et al 1994Gheri et al , 1995Sgambati et al 1995Sgambati et al , 1996. Moreover, early studies of changes in mucin expression during the development of the proventriculus and gizzard focused on alterations in the carbohydrate chain.…”

Section: Discussionmentioning

confidence: 99%

The differences between the localizations of MUC1, MUC5AC, MUC6 and osteopontin in quail proventriculus and gizzard may be a reflection of functional differences of stomach parts

2010

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Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer and are produce by many epithelial tissues in vertebrates. Osteopontin (OPN) is an adhesive phosphorylated glycoprotein that is expressed by a broad range of tissues and cells. Although gastric mucins MUC1, MUC5AC, MUC6 and OPN have been widely used in histological studies and in diagnostic pathology in order to diagnose gastric carcinomas, their localizations in the stomach of quail have not yet been studied. In this study, the localizations of MUC1, MUC5AC, MUC6 and OPN in the proventriculus and gizzard of Japanese quail during the post-hatching period were compared at light microscope levels by applying immunohistochemical methods. In all ages studied, the immunoreactivity of MUC5AC was present in the lining epithelium of both folds and superficial proventricular glands in the proventriculus, whereas MUC1, MUC6 and OPN reactivity was found in the oxynticopeptic cells of profound proventricular glands. In addition, some cells in the fold epithelium of the proventriculus showed a positive reaction to OPN. The immunoreactivity of MUC1 in gizzard was different from that of MUC5AC. Although MUC5AC was expressed in the cells of both the surface epithelium and profound glands of the gizzard, MUC1 was only localized in the profound glands of the gizzard. However, MUC6 and OPN immunoreactivity was absent in the gizzard. The results indicated that the differences between the localizations of MUC1, MUC5AC, MUC6 and OPN in quail proventriculus and gizzard may be a reflection of functional differences of stomach parts. Although the biological significances of the expressions of MUC1, MUC5AC, MUC6 and OPN in the quail stomach remains unknown, these notable glycoproteins may be associated with barrier function, host defence, and ⁄ or secretion.

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