Histochemical demonstration of mucosubstances in the mouse gastrointestinal tract treated with Origanum hypericifolium O. Schwartz and P.H. Davis extract

Abstract

Glycoconjugate-containing mucous in the gastrointestinal tract has lubricant and cytoprotective effects. This study was performed to demonstrate the intensity of mucous in the stomach, small and large intestine tissues of female BALB/c mice injected intraperitoneally with 0.2 ml of the Origanum hypericifolium extract for six weeks. The staining procedures employed were Alcian Blue (AB) at pH 1 and 2.5, periodic acid-Schiff (PAS) and lectins including Galanthus nivalis agglutinin (GNA), Arachis hypogaea (PNA, Peanut Agglutinin) and Maackia amurensis leucoagglutinin (MAL). The mucous glands in the large intestine of the experimental groups were found to show stronger positive reaction for AB at pH 2.5 and PAS when compared with the controls. In lectin histochemistry, the mucous glands which reacted with lectins exhibited less density for PNA in the stomach and for MAL and PNA in the small intestine in the experimental groups than the controls. In the large intestine of the experimental groups, less staining pattern of GNA was observed on the surface epithelium of villi when compared with the controls. The results suggest that O. hypericifolium extract increases acidic and neutral mucosubstances in the large intestine and also changes some glycan structures in the gastrointestinal tract.

Carbohidrate Distribution In The Small Intestine of Sumba Ongole Cattle (Bos indicus)

“…Karbohidrat memiliki peran yang pentingdalam proses metabolisme, proses interaksiantarsel, migrasisel, maturasisel, differensiasi, dan respon imun (Suvarna et al 2019). Perbedaan variasi kandungan karbohidrat pada duodenum, jejunum, dan ileum diduga erat kaitannya dengan sekresi mukus setiap sel serta fungsinya.Menurut Keskin et al (2012), karbohidrat asam pada usus memiliki fungsi dalam proteksi dengan memberikan perlindungan pada mukosa dari dehidrasi dan kerusakan mekanis dan merupakan barier antara epitel untuk melindungi dari partikel makanan dan bakteri patogen, virus, dan parasit, sedangkan fungsi dari karbohidrat netral adalah menetralisasi dan menjaga mukosa saluran pencernaan dari kelebihan asam lambung (HCl) (Novelina, 2003).…”

Histopathological and Histochemical Study of Intestinal Cryptosporidiosis in Mice

“…Gastric and intestinal mucins have been investigated biochemically and histochemically and the changes in their composition have been reported in disease (8). Mucous are composed mainly of layer glycoproteins called mucin and inorganic salt, glycoproteins serve as antioxidative properties in the gastrointestinal tract since structural changes in mucous can lead to some gastrointestinal disease (9). The studies on the changes of mucosubstances in the gastrointestinal tract (histochemically) in pathological problems have great importance.…”