Histochemical Demonstration of Lipase Activity in the Gastric Mucosa of the Cat

Abstract

The aim of the present study was to determine, histochemically, the onset and location of production of preduodenal lipase in fetal, suckling, weaned and adult cats. Strong enzymatic activity was localized in the surface mucous cells of the gastric mucosa in animals at postpartal day 1 after ingestion of milk. Activity of gastric lipase persisted as long as animals were nursed. No gastric lipase could be demonstrated in weaned and adult cats. Lingual lipase was not found at any developmental stage examined. Thus, in the newborn cat, lipase of the gastric mucosa is responsible for milk fat lipolysis.

Periods and Stages of the Prenatal Development of the Domestic Cat

“…(Fig. 22) Literature descriptions include: 120–125 mm (Sainmont, 1906), 83.5–131 g (Tiedemann and Ooyen, 1978), haematocrit 47 (Ooyen, 1977), neuroepithelial bodies in the mature lung (Vanlommel and Lauweryns, 1993), plexus myentericus differentiated (Lolova, 1983), 140 mm (Schnorr, 1989), alveolar stage of the lung (Glöckel, 1984), mature, thick hair coat, first alveolar ducts (Günther, 1994), bronchial glands differentiated (Höfliger and Stünzi, 1975), 60–63 birth, carotid body differentiated (Clarke and Daly, 1984, 1985), first retinal vascularization (Halasz and Stone, 1984), epiglottal taste buds in differentiation (Stedman et al, 1983), gastric lipase detectable (Knospe and Plendl, 1997), before birth the lip gland increases in size, after birth it is reduced (Claassen, 1930; Künzel and Knospe, 1986), formation of the colon ascendens (Krüger, 1929), mammary gland with three to five primary buds, nipples with reduced hairs (Gisler, 1922).…”

Activities of gastric, pancreatic, and intestinal brush-border membrane enzymes during postnatal development of dogs

“…51 Colostrum has lower lipase activity, compared with the activity for milk, and this is consistent with the lower activity measured in the contents of the stomach and small intestine at day 1, compared with activity measured at day 21. Lipase is also secreted by the gastric mucosa of suckling dogs 29,52 and cats, 53 and 10 to 30% of milk fat is hydrolyzed in the stomach of suckling rats. 54 Gastric lipases can be differentiated from those secreted by the pancreas on the basis of stereospecificity 55 and optimal pH.…”

Establishment of culture systems of human gastric epithelium for the study of pepsinogen and gastric lipase synthesis and secretion

“…Furthermore, the Matrispersegenerated epithelial cell cultures consist of mucoustype (PAS staining), parietal (H ϩ -K ϩ ATPase) as well as a significant population of chief cells (Bowie staining), indicating that the monolayer is truly representative of the intact gastric epithelium. The latter feature is particularly important since our understanding of the regulatory mechanisms of human chief cell functions has remained fragmentary because available animal models either lack gastric lipase [rodents (Hamosh et al, 1979)] or have both digestive enzymes located in different cell types [dog (Carriére et al, 1992), cat (Knospe and Plendl, 1997), rabbit (Bernadac et al, 1991)]. Herein, we demonstrate that human chief cells in primary culture retain their capacity to express and efficiently secrete pepsinogen (Pg5) and gastric lipase (HGL).…”