Histochemical demonstration of gamma glutamyl transpeptidase activity in bone marrow and blood cells

Abstract

SYNOPSIS The demonstration by a histochemical method of gamma glutamyl transpeptidase is described. In marrow and peripheral blood it has been found useful in differentiating between normal and leukaemic myeloblasts and between marrow (short-lived) lymphocytes and lymph node (long-lived) lymphocytes. The method may also be of value in differentiating between normal and neoplastic cells.

Activity Determination, Kinetic Analyses and Isoenzyme Identification of Gamma Glutamyltransferase in Human Neutrophils

“…It is also present in various body fluids, such as saliva, serum, bile and urine (Nemesanzky and Lott, 1985;Sener, 1997). GGT has been detected both in blood and bone marrow cells by using cytochemical methods (Szmigielski et al, 1965). It has been demonstrated at the ultrastructural level on the surface membrane and membranes of endoplasmic reticulum and golgi apparatus of human lymphoid cells (Marathe et al, 1980).…”

<i>L</i>-<i>γ</i>-Glutamyl Transpeptidase Activity in Normal and Leukemic Leukocytes

“…/-Glutamyl transpeptidase has formerly been histochemically demonstrated in mye loid elements of the bone marrow, including mitotic normoblasts and megakaryocytes [7]. The /-glutamyl transpeptidase activity of the marrow lymphocytes was also shown to be significantly lower than that of the lymph node lymphocytes.…”

Cytochemical reactions of normal and neoplastic lymphocytes.

“…In contrast, others (Barr and Perry, 1976) have noted negative or weak acid phosphatase activity in normal human peripheral blood T lymphocytes and moderate to strong reactivity in B Received for publication 8 December 1976 and null cells. In addition, other investigators (Szmigielski et al, 1965) have shown that 75% of human peripheral blood lymphocytes and 2-10% of bone marrow lymphocytes contain gamma-glutamyl transpeptidase activity. Since T cells have a similar distribution in the blood and bone marrow, it seemed possible that gamma-glutamyl transpeptidase was also a cytochemical marker for T cells.…”

“…In addition, other investigators (Szmigielski et al, 1965) have shown that 75% of human peripheral blood lymphocytes and 2-10% of bone marrow lymphocytes contain gamma-glutamyl transpeptidase activity. Since T cells have a similar distribution in the blood and bone marrow, it seemed possible that gamma-glutamyl transpeptidase was also a cytochemical marker for T cells.…”

“…Ten samples from normal individuals were stained for the presence of beta-glucuronidase (Lorbacher et al, 1967), PAS (Hayhoe and Flemans, 1969), and gamma-glutamyl transpeptidase (Szmigielski et al, 1965). In addition, 12 samples were stained for acid phosphatase (Li et al, 1970) and 12 different samples were stained for alpha-naphthyl butyrate esterase (Li et al, 1973) Portions of tissue were gently pressed through a fine stainless steel mesh and a cell suspension was washed three times in phosphate buffered saline solution at pH 7 0.…”