Histochemical demonstration of acid mucopolysaccharide production by tissue cultured epithelial-like cells

Sisca, R.F.; Langkamp, H.H.; Thonard, John C.

doi:10.1007/bf00264390

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…The intercellular substances of gingival epithelium consist predominantly of proteoglycans and glycoproteins (Sisca et al, 1971; Macromolecular material extracted with 4M-guanidinium chloride was applied to a column of Sepharose 2B-CL and eluted with 0.5 M-sodium acetate at pH 6.8 (a). The excluded peak (E) was then fractionated under dissociative conditions in 4M-guanidinium chloride at pH 5.8 (b).…”

Section: Resultsmentioning

confidence: 99%

Proteoglycans from adult human gingival epithelium

Wiebkin¹,

Bartold²,

Thonard³

1979

Biochemical Journal

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Section: Resultsmentioning

confidence: 99%

Proteoglycans from adult human gingival epithelium

Wiebkin¹,

Bartold²,

Thonard³

1979

Biochemical Journal

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“…When epithelial cell cultures are grown to high cell density, i.e. to confluence, the matrix material surrounding the cells is confined to form intercellular matrix which can be his- tochemically stained for polyanions (Sisca, Langkamp & Thonard 1971, Thonard & Wiebkin 1973. By differential elimination of staining with appropriate enzymes, the major polyanionic components of the intercellular substances appear to have glycosaminoglycan properties.…”

Section: Discussionmentioning

confidence: 99%

Mucopolysaccharide localization in gingival epithelium

Wiebkin

Thonard

1981

J of Periodontal Research

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Short incubations, at 37°C, of small freshly excised pieces of healthy human gingivae iancorporated [35S]‐sulphate and [3H]‐acetate into macromolecular material which could be precipitated intercellularly in the epithelium. Following radioactively pulse‐chased incubations, such localization was observed by autoradiography on cryostat histological sections fixed in cetylpyridinium chloride. Critical electrolyte salt concentration elution indicated that most of the intercellular material was soluble in 0.63M‐MgCl2, and any material which remained was intracellular. In vitro and in vivo incorporation studies were compared. These data corroborate biochemical studies (Wiebkin, Bartold & Thonard 1979) together with other histochemical observations that proteoglycans (mucopolysaccharides) are a major intercellular component of human gingival epithelium. Molecular conformation and the relatively rapid synthesis and secretion rate for this class of epithelial macromolecule may explain the lack of susceptibility of this material in the intercellular site, both to degradation by some specific enzymes previously reported and to elution with critical salt concentrations from cationic detergent precipitates. The method described, together with in vivo incorporation studies, provides a useful technique for studying direct effects of some microenvironmental influences on gingival epithelium.

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“…Alcian Blue, a dye that binds acid mucopolysaccharides, is also used for Hyaluronic acid staining [15]. This procedure has evidenced Hyaluronan overexpression in different melanoma variants [16][17][18].…”

Section: Hyaluronic Acid Overexpression In Tumorsmentioning

confidence: 99%

Hyaluronidase and other Extracellular Matrix Degrading Enzymes for Cancer Therapy: New Uses and Nano-Formulations

Scodeller¹

2014

J Carcinog Mutagen

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Overexpression of extracellular matrix components in tumor stroma like Hyaluronan and collagen pose a hurdle for cancer therapies. Many tumors overexpress Hyaluronic acid (HA), and Collagen. The overexpression of these components results in the elevation of a parameter called interstitial fluid pressure, which represents the resistance to the flow of a liquid. High molecular weight HA and Collagen bind water very tightly, and thus "wetting" these tumors with the drug-containing solution is impeded because it demands the displacement of those tightly bound water molecules solvating these polymers. When using conventional chemotherapy to treat a tumor, the penetration of the drug to the tissue is impoverished due to this phenomenon. This review will collect new experimental approaches for degrading the extracellular matrix with enzymes, and the use of this as an adjuvant for conventional cancer drugs. Adjuvant systems composed of free enzyme in solution or immobilized enzyme on the surface of nanoparticles or expressed by a viral vector will be dealt with.

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Histochemical demonstration of acid mucopolysaccharide production by tissue cultured epithelial-like cells

Cited by 5 publications

References 12 publications

Proteoglycans from adult human gingival epithelium

Proteoglycans from adult human gingival epithelium

Mucopolysaccharide localization in gingival epithelium

Hyaluronidase and other Extracellular Matrix Degrading Enzymes for Cancer Therapy: New Uses and Nano-Formulations

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