Histochemical changes in the tooth and jaw germs of laboratory rats under the influence of ecotoxicants and during perinatal prevention

Abstract

Relevance. Unreasonably high pollutant emissions into the environment, the ill-considered use of natural resources and the raw material orientation of exports have led to an environmental crisis in many areas.Purpose. To study the effect of ecotoxicants on the histochemical structure of tooth and jaw germs in laboratory rats and ways to reduce this effect during perinatal prevention.Materials and methods. The experiment included 50 white outbred rats weighing 180-250 grams. All animals formed five groups: 1 control and four experimental. All animals of the experimental groups were subject to gasoline and formaldehyde vapours inhalation exposure; Group 1 (control) was subject to only poisoning with ecotoxicants, Group 2 had peptinsorbent associated with ecotoxicant poisoning, Group 3 – a membrane protector – magnolia vine, Group 4 – beet, Group 5 – peptinsorbent, membrane protector and beet.Results. The histochemical study of tissues around the tooth germs in pup rats born from the control group rats after poisoning with ecotoxicants revealed an increased mast cell number in the tissues compared to intact rats. The samples of Group 2 rats showed that the mast cell number and characteristics practically did not differ from the control group after ecotoxicant poisoning. In the rats of Group 3, the number of mast cells was slightly lower than in the control group. In Group 4, the number of mast cells was not significantly lower than in the control group. In the rats of Group 5, the number of mast cells significantly decreased compared to the control and other experimental groups.Conclusion. Thus, during subchronic poisoning of pregnant female rats with ecotoxicants, the number of mast cells in the gingiva of rat pups increases against the background of microcirculation disorders. The number of mast cells in gingiva significantly decreases and approaches the normal values in rat pups after feeding pregnant rats with the combined mixture (peptinsorbent+membrane protector Schisandra+beetroot).