Histochemical and ultrastructural changes of sternomastoid muscle in aged wistar rats

Ciena, Adriano Polican; Almeida, Sonia Regina Yokomizo de; Alves, Paulo Henrique de Matos; Bolina-Matos, Regina de Sousa; Días, Fernando José; Issa, João Paulo Mardegan; Iyomasa, Mamie Mizusaki; Watanabe, Ii-sei

doi:10.1016/j.micron.2011.06.003

Cited by 17 publications

(16 citation statements)

References 31 publications

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“…The tongues ( n = 2) were fixed in a modified Karnovsky solution (containing 2.5% glutaraldehyde and 2% paraformaldehyde in sodium phosphate buffer solution to 0.1 M [pH = 7.4]) according to Watanabe and Yamada () and Ciena et al. (), and 3‐mm 3 fragments from the dorsal epithelium of the tongue were retrieved. Samples were postfixed in an 1% osmium tetroxide solution, at 4°C and in 5% uranyl acetate water solution at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Morphological characteristics of the papillae and lingual epithelium of guinea pig (Cavia porcellus)

et al. 2017

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The structural elements, tridimensional aspects of the lingual papillae, yours connective tissue cores (CTCs) and the ultrastructure of the dorsal epithelium surface of the tongue of guinea pig are examined. Filiform papillae and their CTCs lie throughout the tongue surface, featuring subtypes and different shapes. Fungiform papillae are dome-shaped, and their CTCs are few, with heterogenic disposition among the filiform papillae in the lingual apex and body and in grooves stretching to the side margin among the foliate papillae towards the lingual apex. Foliate papillae are well developed, and their CTCs are composed of five pairs of parallel seams limited by deep grooves placed bilaterally in the caudal region. Two vallate papillae are observed in the caudal region and reveal specialized structures which are developed and differentiated when they present compound elements by a double epithelial projection, in a column, similar to the shape of the foliate papilla. Further, the epithelium is organized by layers with ultrastructural components. Therefore, we conclude that the structural descriptions, dimensional aspects of tongue papillae, their CTCs and the ultrastructure of the tongue epithelium of guinea pigs are similar to other rodent mammals, however, with new descriptions as double epithelial projection constituting each vallate papilla.

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Section: Methodsmentioning

confidence: 99%

Morphological characteristics of the papillae and lingual epithelium of guinea pig (Cavia porcellus)

et al. 2017

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“…After selecting the area of interest, ultra‐thin sections of 60 nm were obtained, which were collected in 200 mesh copper screens (Sigma‐Aldrich, USA). The sections were contrasted in 4% uranyl acetate solution for 3 min, washed with distilled water, and then contrasted in an aqueous solution of 0.4% lead citrate for 3 min (Ciena et al, ; Ciena, De Almeida, Dias, et al, ). The screens were examined through the Philips Transmission Electron Microscopy (TEM) CM 100, Microscopy Laboratory of the Institute of Biosciences, UNESP.…”

Section: Methodsmentioning

confidence: 99%

“…The MTJ region present in the distal extremities of the skeletal musculature in relation to the muscular belly has various adaptations with respect to syntopy, function, disposition, topography, and histochemical composition. The main function is to transmit the strength generated from the musculature to the tendinous tissue that is capable of triggering several morphological and functional alterations in the myotendinous structure (Ciena et al, ; Ciena, De Almeida, Dias, et al, ).…”

Section: Introductionmentioning

confidence: 99%

Myotendinous junction plasticity in aged ovariectomized rats submitted to aquatic training

Sierra

Fávaro

Cerri

et al. 2018

Microscopy Res & Technique

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The study aims to describe the tissue plasticity of MTJ through the morphological analysis of MTJ soleus in ovariectomized aged female Wistar rats submitted to aquatic training. Forty aged Wistar rats, 1 year and 2 months of age, were divided into four groups: sedentary (S), trained (T), ovariectomized (O), and trained/ovariectomized (OT). Employing the transmission electron microscopy, the ultrastructural and morphometric elements were revealed. In the S group, changes in morphological characteristics as a consequence of the aging process were seen, demonstrated by the conical shape of the muscle cell extremity, a large area with collagen deposit, and misalignment of sarcomeres in series. The T group presented ample adjustments when revealed the organization of MTJ, through the increase of the contact area and greater lengths of sarcoplasmatic invaginations and evaginations. The O group revealed extensive tissue disorganization with muscle atrophy, reduction of MTJ contact area, and consequently, changes in sarcoplasmatic invaginations and evaginations. The OT group demonstrated extensive remodeling with restructuring MTJ through the increase of tissue contact area, extensive organization, parallel arrangement, and increased length of sarcoplasmatic invaginations and evaginations. The distal sarcomeres presented higher lengths compared to the proximal sarcomeres in both the groups. We conclude that aquatic training was effective in the organization and structural remodeling of the myotendinous interface of ovariectomized aged rats. There was a greater area of contact, and consequently, greater resistance in the myotendinous interface promoting a lower predisposition to injuries.

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“…Animals were anesthetized using the same conditions, perfused, and fixed with modified Karnovsky solution containing 2.5% glutaraldehyde and 2% paraformaldehyde in sodium phosphate buffer 0.1M (Ciena et al,2011). Tissues were removed and sectioned in small blocks, fixed in the same solution for overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Biophysical characterization of polymyxin b interaction with LPS aggregates and membrane model systems

Domingues¹,

Inácio²,

Raimundo³

et al. 2012

Biopolymers

130

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Antibiotic resistance is an increasingly severe health problem. Antimicrobial peptides (AMPs) are being developed in order to overcome this problem, due lower bacterial resistance. Polymyxin B is an AMP with bactericidal effect on Gram-negative bacteria due to its high affinity for lipopolysaccharide (LPS). The objective of this work was to unravel the polymyxin B mechanisms of LPS neutralization and bactericidal activity. Using dynamic light scattering, it was observed that polymyxin B induces LPS aggregation in a concentration-dependent manner. The peptide increases the surface charge of LPS and membrane model systems, as revealed by zeta-potential measurements. The higher zeta-potential variations were detected in the presence of the negatively charged POPG membranes. This higher interaction with negatively charged membranes, made of POPG, was followed at higher peptide concentration by membrane permeabilization. Also, for zwitterionic POPC membranes a higher membrane leakage was detected. The peptide promotion of LPS aggregation may be related with the clearance of LPS from the bloodstream, eventually by facilitating macrophage phagocytosis and/ or blocking the binding of LPS to its receptor. Our data indicate that polymyxin B mechanism of action at the molecular level involves a first step of electrostatic approach toward LPS; then, it may be internalized and bind to the bacterial phosphatidylglycerol-rich membrane leaflets, inducing leakage at higher peptide concentrations.

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Histochemical and ultrastructural changes of sternomastoid muscle in aged wistar rats

Cited by 17 publications

References 31 publications

Morphological characteristics of the papillae and lingual epithelium of guinea pig (Cavia porcellus)

Morphological characteristics of the papillae and lingual epithelium of guinea pig (Cavia porcellus)

Myotendinous junction plasticity in aged ovariectomized rats submitted to aquatic training

Biophysical characterization of polymyxin b interaction with LPS aggregates and membrane model systems

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