Histochemical and SEM evaluation of the neuromuscular junctions from alcoholic rats

Torrejais, Márcia Miranda; Soares, J. C.; Matheus, Selma Maria Michelin; Cassel, F. D.; Mello, Josiane Medeiros de; Basso, N.

doi:10.1016/s0040-8166(02)00023-x

Cited by 5 publications

(4 citation statements)

References 22 publications

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“…Motor in-coordination is a behavioral expression of neuropathic dysfunction due to alteration in the soleus and extensor digitorum longus (EDL) muscles tone [58]. It can be measured by elevating grip strength of neuropathic animals.…”

Section: Discussionmentioning

confidence: 99%

Elucidation of molecular mechanism involved in neuroprotective effect ofCoenzymeQ10 in alcohol‐induced neuropathic pain

Kandhare

Ghosh

Ghule

et al. 2012

Fundamemntal Clinical Pharma

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The aim of the present investigation was to evaluate the effect of Coenzyme Q10 and its combination with vitamin E in alcohol-induced chronic neuropathic pain. Male Wistar rats were orally treated with alcohol (10 g/kg, 35% v/v, b.i.d.) for 10 weeks. Coenzyme Q10 (25, 50, and 100 mg/kg) and vitamin E (100 mg/kg) were coadministered orally for 1 h after ethanol administration for 10 weeks. Various nerve functions, biochemical, and molecular parameters were assessed. Chronic administration of ethanol for 10 weeks resulted significant development of neuropathic pain. Treatment with Coenzyme Q10 (50 and 100 mg/kg) for 10 weeks showed significant and dose dependently increased in level of nociceptive threshold, endogenous antioxidant, and Na,K-ATPase enzyme. Coenzyme Q10 (50 and 100 mg/kg) significantly restored the levels of motor nerve conduction velocity and sensory nerve conduction velocity. It also showed significant decrease in levels of endogenous calcium, oxidative-nitrosative stress, TNF-α, IL-1β, and IL-4 level. Alteration in protein expression of polymerase gamma (pol γ) was significantly restored the Coenzyme Q10 treatment. The important finding of the study is that, Coenzyme Q10 (100 mg/kg) and α-tocopherol (100 mg/kg) combination-treated rats showed more significant prevention of behavioral, biochemical, and molecular neurotoxic effect of alcohol administration than Coenzyme Q10 or α-tocopherol alone treated group. It is evident from the finding of present investigation that plethora of mechanism including inhibition of oxido-nitrosative stress, release of pro-inflammatory cytokine, modulation of endogenous biomarker, and protection of pol γ protein expression simultaneously orchestrate to exhibits neuroprotective effect of Coenzyme Q10, vitamin E and their combination.

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Section: Discussionmentioning

confidence: 99%

Elucidation of molecular mechanism involved in neuroprotective effect ofCoenzymeQ10 in alcohol‐induced neuropathic pain

Kandhare

Ghosh

Ghule

et al. 2012

Fundamemntal Clinical Pharma

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“…3, p. 361-364, 2004 tendínea em ratos, metodologia empregada para verificar o efeito do alcoolismo em outros sistemas, como descrito por Martinez (1993), Cagnon (1996 e Pereira et al (2002). A influência do alcoolismo nos diferentes sistemas do organismo foi descrita por Lieber (1984) e Hirata e Hirata (1991), enquanto que a influência do alcoolismo sobre os músculos foi descrita por autores como (Jordo et al, 1975;Ruben et al, 1976;Levy et al, 1984, Martin e Peters, 1985Levy, 1991;Andreo, 2001;Preedy et al, 2001;Torrejais et al, 2002;Adachi et al, 2003), os quais mencionam que o álcool provoca atrofia precoce, degeneração de fibras musculares, acúmulo e infiltrado inflamatório, atrofia das fibras musculares do tipo II, déficit motor das fibras musculares, podendo levar a sua necrose.…”

Section: Discussionunclassified

“…Nos músculos, sabe-se que o álcool provoca atrofia precoce, degeneração de fibras musculares, acúmulo e infiltrado inflamatório, atrofia das fibras musculares do tipo II, déficit motor das fibras musculares, podendo levar a sua necrose (Jordo et al, 1975;Ruben et al, 1976;Levy et al, 1984;Levy, 1991;Martin e Peters, 1985;Andreo, 2001;Preedy et al, 2001;Torrejais et al, 2002;Adachi et al, 2003).…”

Section: Introductionunclassified

Interface músculo-tendínea de ratos induzidos a ingestão alcóolica

Pereira¹,

Conegero²

2004

Acta Sci. Biol. Sci.

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A ingestão de grandes quantidades de álcool etílico pode afetar os mais variados sistemas do corpo humano. Nos músculos, sabe-se que o álcool provoca atrofia precoce, degeneração e déficit motor das fibras musculares, podendo levar a sua necrose. Objetivamos, neste estudo, analisar a sua influência na interface músculo-tendínea. Para tanto, utilizamos 12 ratos wistar (Rattus norvegicus), divididos em grupo A (controle), tratados com água potável e ração comercial, e B (experimental), que receberam aguardente diluída a 30ºG.L. e ração comercial por um período de 100 dias. Após o sacrifício, coletamos os músculos flexores do membro anterior direito, que foi fixado em solução de Bowin por 48 horas e experimentaram as técnicas de rotina histológica para inclusão em parafina. De acordo com a metodologia empregada, verificamos que houve alterações histológicas, tais como o acúmulo de tecido adiposo na região de interface músculo-tendínea e desestruturação das fibras musculares nos animais experimentais. Podemos inferir que, na dosagem utilizada, o álcool etílico provoca alterações histológicas na interface músculotendínea.

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“…It alters the nervous system, provoking periferic neuropathies and alterations in the axon, glucose metabolism, lipids and proteins, and particularly, alters the nutritional aspects of organs such as: pancreas, stomach and intestine (Korsten and Lieber, 1979;Hirata and Hirata, 1991), early degeneration and atrophy of structures of the muscle esqueletal system (Torrejais et al, 2002;Pereira and Conegero, 2004).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Indução à ingestão de álcool etílico retarda o processo de regeneração pós-fratura em rato

Pereira

Conegero

2007

Acta Sci. Biol. Sci.

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Histochemical and SEM evaluation of the neuromuscular junctions from alcoholic rats

Cited by 5 publications

References 22 publications

Elucidation of molecular mechanism involved in neuroprotective effect ofCoenzymeQ10 in alcohol‐induced neuropathic pain

Elucidation of molecular mechanism involved in neuroprotective effect ofCoenzymeQ10 in alcohol‐induced neuropathic pain

Interface músculo-tendínea de ratos induzidos a ingestão alcóolica

Indução à ingestão de álcool etílico retarda o processo de regeneração pós-fratura em rato

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