Histochemical and morphological study of dystrophic () and normal muscle

Dribin, Lori B.; Simpson, Sidney B.

doi:10.1016/0014-4886(77)90316-8

Cited by 42 publications

(14 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The average ratio of Type I to II was 41:59 in 820 fibres. This is close to the ratio of 39:61 reported for the C57 BL strain in a more extensive investigation by Dribin & Simpson (1977) who used the term 'red' for Type II fibres. In agreement with Dribin & Simpson (1977) there was no significant difference (t = 0-5) between the mean area of Type I (7-7.…”

Section: Resultssupporting

confidence: 57%

“…The distribution of Luff & Atwood's fibre twitch times-to-peak was bimodal, with peaks at 11 and 17 msec. The proportion of fast fibres, however, was about 25 %, which contrasts with the proportion of Type II fibres (varying between 31 % and 61 % for different strains of mice: Vaughan, Aziz-Ullah, Goldspink & Nowell, 1974;Dribin & Simpson, 1977). It is possible that Luff & Atwood's interpretation is incorrect as their two modes are separated by a fall in numbers in only one bin.…”

Section: Discussionmentioning

confidence: 49%

“…Soleus is normally regarded as a slow twitch muscle composed entirely or predominantly of slowly contracting Type I (low myosin ATPase activity) muscle fibres. Mouse soleus, however, is a mixture of both Type I and Type IIa (high myosin ATPase activity, high SDH, oxidative capacity) fibres, and the Type I fibres constitute only 40 % of the adult muscle fibres (Dribin & Simpson, 1977) and even less (about 25 %) in the first post-natal month .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isometric contractions of motor units and immunohistochemistry of mouse soleus muscle.

Lewis¹,

Parry²,

Rowlerson³

1982

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. Isometric contractions of motor units, isolated functionally by ventral root splitting in vivo, were recorded from mouse soleus muscle.2. Motor unit tensions varied over a narrow symmetrical range and averaged 4-7 % of whole muscle tension, corresponding to twenty-one motor units per muscle.3. There was considerable variation between muscles in isometric twitch timesto-peak and even greater variation for the motor units. The distribution of motor unit times-to-peak was apparently unimodal and could be fitted by a single normal population. A slightly better fit was, however, obtained with two normal populations, as suggested by the histochemistry.4. Twitch time-to-peak decreased in proportion to axonal conduction velocity in individual animals. The whole population of motor units could be fitted by a linear relation between time-to-peak and the reciprocal of conduction time in the motor axon. Motor unit tension was also linearly related to the reciprocal ofconduction time.5. Histochemistry showed clear division between Type I and Type IIa fibres. Type I fibres reacted strongly with antibody against slow myosin of cat soleus muscle; Type II a gave a reaction no stronger than the background. The division was as clear as in the cat or rat.

show abstract

Section: Resultssupporting

confidence: 57%

Section: Discussionmentioning

confidence: 49%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isometric contractions of motor units and immunohistochemistry of mouse soleus muscle.

Lewis¹,

Parry²,

Rowlerson³

1982

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…In accordance with previous reports, we find only one fibre type in the guinea-pig soleus muscles using the histochemical myosin ATPase assay, whereas the mouse and rat soleus are mixed muscles, containing type I and type IIA fibres (Close, 1967;Ariano, Armstrong & Edgerton, 1973;Lewis, Parry & Rowlerson, 1982). The proportion of type I fibres in mouse soleus in markedly higher than that reported in other works, probably because the strain used here (NMRI) is different (C57BL/6J in Dribin &Simpson, 1977 andin Anderson et al 1988). One isomyosin, SM2, is present in the soleus muscles of all three species; this form migrates very slowly (electrophoretic mobility= 100).…”

Section: Statistical Analysescontrasting

confidence: 34%

Maximal shortening velocities, isomyosins and fibre types in soleus muscle of mice, rats and guinea‐pigs.

Asmussen

Maréchal

1989

The Journal of Physiology

View full text Add to dashboard Cite

3. In guinea-pig soleus, V0 is equal to the maximal velocity (Vmax) computed using the Hill force-velocity relationship; V0 is approximately twice as large as Vmax in mouse and rat soleus.4. V0 measures the unloaded shortening velocity of the fastest fibres whereas Vmax is a function of the force-velocity characteristics of all the fibres contained in the muscle.5. V0 increases according to the isomyosin composition of the fibres in the sequence SM2 < SM1+ IM < IM.

show abstract

“…Dribin and Simpson (1977) noted in their study of dy2Jldy2J animals that the slowtwitch soleus and the "red portion" of the fast-twitch gastrocnemius were more severely dystrophic than the prevailing "white" portion of the latter. They also reported that solei in this strain exhibited decreased numbers of "intermediate" fibers when compared to their normal counterparts.…”

mentioning

confidence: 96%

Abnormal distribution of fiber types in the slow‐twitch soleus muscle of the C57BL/6J DY^2J/DY^2J dystrophic mouse during postnatal development

et al. 1983

View full text Add to dashboard Cite

The postnatal development of extrafusal fibers in the slow-twitch soleus muscle of genetically dystrophic C57BL/6J dy2J/dy2J mice and their normal age-matched controls was investigated by histochemical and quantitative methods at selected ages of 4, 8, 12, and 32 weeks. The majority of fibers in the soleus consisted of two kinds, fast-twitch oxidative-glycolytic (FOG) and slow-twitch oxidative (SO), according to reactions for alkaline-stable and acid-stable myosin ATPase and the oxidative enzyme, NADH-tetrazolium reductase. A minor population of fibers, stable for both alkaline- and acid-preincubated ATPase, but variable in staining intensity for NADH-TR, were designated "atypical" fibers. With age, the normal soleus exhibited a gradual increase in the number and proportion of SO fibers and a reciprocal, steady decline in the percentage of FOG fibers. Atypical fibers were numerous at 4 weeks, but were substantially diminished at later ages. Since total extrafusal fiber number remained relatively constant between the periods examined, this change in relative proportions reflects an adaptive transformation of fiber types characteristic of normal postnatal growth. A striking alteration in the number and distribution of fiber types was associated with the dystrophic soleus. At 4 weeks an 18% reduction in total fiber number was already noted. Subsequently, by 32 weeks a further 22% diminution in overall fiber number had occurred. With age, the absolute number and proportion of dystrophic SO fibers were drastically reduced. In contrast, the percentage of dystrophic FOG fibers increased significantly while their absolute numbers between 4 and 32 weeks remained relatively constant. Atypical fibers in the dystrophic solei were found in elevated numbers at all age groups, particularly at 12 weeks. They may, in part, represent attempts at regeneration or an intermediate stage in fiber-type transformation. Microscopically, both of the major fiber types appeared affected, albeit differently, by the dystrophic process. We suggest that a failure or retardation in the normal postnatal conversion of fiber types within the soleus muscle occurs in this murine model for muscular dystrophy.

show abstract

Histochemical and morphological study of dystrophic () and normal muscle

Cited by 42 publications

References 30 publications

Isometric contractions of motor units and immunohistochemistry of mouse soleus muscle.

Isometric contractions of motor units and immunohistochemistry of mouse soleus muscle.

Maximal shortening velocities, isomyosins and fibre types in soleus muscle of mice, rats and guinea‐pigs.

Abnormal distribution of fiber types in the slow‐twitch soleus muscle of the C57BL/6J DY^2J/DY^2J dystrophic mouse during postnatal development

Contact Info

Product

Resources

About

Histochemical and morphological study of dystrophic () and normal muscle

Cited by 42 publications

References 30 publications

Isometric contractions of motor units and immunohistochemistry of mouse soleus muscle.

Isometric contractions of motor units and immunohistochemistry of mouse soleus muscle.

Maximal shortening velocities, isomyosins and fibre types in soleus muscle of mice, rats and guinea‐pigs.

Abnormal distribution of fiber types in the slow‐twitch soleus muscle of the C57BL/6J DY2J/DY2J dystrophic mouse during postnatal development

Contact Info

Product

Resources

About

Abnormal distribution of fiber types in the slow‐twitch soleus muscle of the C57BL/6J DY^2J/DY^2J dystrophic mouse during postnatal development