Histochemical and immunohistochemical study on endocrine cells (5HT, GAS, and SST) of the gastrointestinal tract of a teleost, the characin Astyanax bimaculatus

Cardoso, Nathália das Neves; Firmiano, Enely Maris da Silveira; Gomes, Iracema David; Nascimento, Aparecida Alves do; Sales, Armando; Araújo, Francisco Gerson

doi:10.1016/j.acthis.2015.05.007

Cited by 19 publications

(17 citation statements)

References 35 publications

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“…mesopotamicus , of the same family of Tambaqui Serrasalmidae (da Cruz, Moretti, Nordi, Cyrino, & Machado‐Neto, ), except for A . bimaculatus (Cardoso et al, ) and T . obscrus (Hur et al, ) that showed assorted distribution of GCs (Table ).…”

Section: Discussionmentioning

confidence: 99%

Distribution of goblet and endocrine cells in the intestine: A comparative study in Amazonian freshwater Tambaqui and hybrid catfish

Pereira

Nebo

Naves

et al. 2019

Journal of Morphology

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Goblet cells (GCs) and endocrine cells (ECs) play an important role in intestine physiology, and few studies currently exist for Amazonian fishes. This study aimed to quantify the distribution of GCs and ECs producing cholecystokinin‐8 and neuropeptide Y, assessed by mucin histochemistry and peptides immunohistochemistry, in the intestine of two Amazonian species with different feeding habits Tambaqui (Colossosoma macropomum) and hybrid catfish (Pseudoplatystoma reticulatum × Leiarius marmoratus), an omnivore and carnivore, respectively. A systematic literature review correlating feeding habit and GC and EC distribution was also included to contribute to the comparative study. The results of this study provided novel information about the gut cells of Tambaqui and hybrid catfish. Both, GCs and ECs can be found sweeping the entire intestine of Tambaqui and hybrid catfish although the cells can be more concentrated in certain segments. The GCs and ECs in Tambaqui were more uniformly distributed in the midgut segments (T1, T2, and T3). Unlike, in hybrid catfish GCs were more concentrated in the hindgut (C4) and ECs mainly in the two midgut segments (C1 and C2) of hybrid catfish. Based on the comparison between Tambaqui, hybrid catfish, and other fishes in the literature review, we suggest that cell distribution can be partially explained by feeding habits, carnivorous vs. omnivorous.

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Section: Discussionmentioning

confidence: 99%

Distribution of goblet and endocrine cells in the intestine: A comparative study in Amazonian freshwater Tambaqui and hybrid catfish

Pereira

Nebo

Naves

et al. 2019

Journal of Morphology

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“…Neutral mucins help in lubrication and, when predominant in the oesophagus, indicate pre‐gastric digestion (Cao & Wang, ). Unlike various species of Siluriformes and Characiformes (Cardoso et al, ; Faccioli et al, ; Ghosh & Chakrabarti, , ; Raji & Norouzi, ) in which histochemical reaction of the pyloric region of the stomach was found to be intense only for neutral mucous substances, P. brachypomus presented a strong histochemical response to all of the stains applied, thus demonstrating a greater chemical diversity of mucous substances. This result was similar to what was reported for two species of Anguilliformes (Domeneghini et al, ; Takiue & Akiyoshi, ).…”

Section: Discussionmentioning

confidence: 86%

The digestive tube of Piaractus brachypomus: gross morphology, histology/histochemistry of the mucosal layer and the effects of parasitism by Neoechinorhynchus sp.

Oliveira

Matos

Silva

et al. 2019

Journal of Fish Biology

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The objective of the present study was to describe the histology and histochemistry of the mucosal layer of the digestive tube of Piaractus brachypomus, and the histopathology associated with parasitism by Neoechinorhynchus sp. The digestive tube of P. brachypomus consists of three macroscopically distinct portions: short, rectilinear and elastic‐walled ooesophagus, J‐shaped siphon stomach and a long intestine with rectilinear and curved portions, defined by patterns of villi as foregut, midgut, and hindgut. Histological and histochemical differences were observed in the mucosal layers of the different digestive tube regions, such as intense production of neutral and acidic mucous substances in the pseudostratified mucosal epithelium of the oesophagus; positive periodic acid Schiff reagent (PAS)reactions at the apex of the columnar epithelial cells of the stomach and increased intensity of histochemical reactions in the hindgut region. Neoechinorhynchus sp. was present in 85.7% of specimens examined, with a mean intensity of 7.4 ± 6.2 (±) and abundance of 6.33. Good health of the fish indicated by high relative condition factor values ( Kn) and occurrence of only mild to moderate alteration in the mucosal layer indicated that Neoechinorhynchus sp. exhibits low pathogenicity towards P. brachypomus hosts in farming environments, with low levels of infection.

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“…In the present study, the finding that type II cells were more abundant in the posterior than the anterior intestine may indicate that the posterior intestine plays a functional role in the absorption of macromolecular proteins and assimilation of proteins for both digestive and defensive purposes (Vernier & Mellinger, ; Domeneghini et al ., ). Type III cells secrete acid glycoconjugates and neutral glycoconjugates and their functions overlap with those of type I as well as type II cells (Buchmann & Bresciani, ; Fiertak & Kilarski, ; Díaz et al ., ; Petrinec et al ., ; Cardoso et al ., ). The present study observed the lowest total number of type III cells in the gastrointestinal tract of C. semilaevis .…”

Section: Discussionmentioning

confidence: 97%

“…Non-specific esterase (NSE) plays a crucial role in ester and protein metabolism, whereas peroxidase (POD), which is often found in peroxisomes, protects cells against harmful substances by converting these into non-toxic materials. The mucous cells secrete a muco-substance that lubricates and increases digestive efficiency of food, as well as protecting the mucosa from proteolytic damage and regulating pH (Gisbert et al, 1999;Domeneghini et al, 2005;Díaz et al, 2008;Cardoso et al, 2015). The mucus of goblet cells, in addition to the glycocalyx and brush border enzymes of enterocytes such as ALP, protects the organism from invading pathogenic microorganisms (Liu et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Histochemical distribution of four types of enzymes and mucous cells in the gastrointestinal tract of reared half‐smooth tongue sole Cynoglossus semilaevis

Wang

Sun

et al. 2017

Journal of Fish Biology

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The histochemical distribution of acid phosphatase (ACP), alkaline phosphatase (ALP), non-specific esterase (NSE), peroxidase (POD) and mucous-cell types was evaluated in the gastrointestinal tract of the half-smooth tongue sole Cynoglossus semilaevis. The enzymes were detected in the entire stretch of the gastrointestinal tract. ACP activity was found in the supranuclear region of enterocytes and the lamina propria of the intestine, as well as the cytoplasm of epithelial cells of the stomach. The staining intensity of ACP in the anterior and posterior intestines was stronger than in the stomach. ALP activity was detected in the striated border of enterocytes and muscularis of the whole intestine, lamina propria and supranuclear cytoplasm of the enterocytes in the anterior intestine, as well as in the blood vessels of the stomach. The staining intensity for ALP in the anterior intestine was stronger than in the posterior segment and the latter was stronger than in the stomach. NSE activity was detected in the cytoplasm of the epithelial cells in the entire gastrointestinal tract, with the anterior intestine showing stronger intensity than the stomach. POD activity was located in the blood cells of the lamina propria of the gastrointestinal tract and the levels in the stomach were similar to the anterior and posterior intestines. Alcian blue (pH 2·5) periodic acid Schiff (AB-PAS) histochemical results revealed three types of mucous cells in the gastrointestinal tract. Type I cells (PAS+AB-) were observed among the gastric mucosa columnar cells in the stomach and enterocytes in the basal region of the villi and in the middle and top regions of the intestinal villi. Type II cells (PAS-AB+) and type III cells (PAS+AB+) were not detected in the stomach but were distributed ubiquitously among enterocytes in the middle and top regions of the intestinal villi.

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Histochemical and immunohistochemical study on endocrine cells (5HT, GAS, and SST) of the gastrointestinal tract of a teleost, the characin Astyanax bimaculatus

Cited by 19 publications

References 35 publications

Distribution of goblet and endocrine cells in the intestine: A comparative study in Amazonian freshwater Tambaqui and hybrid catfish

Distribution of goblet and endocrine cells in the intestine: A comparative study in Amazonian freshwater Tambaqui and hybrid catfish

The digestive tube of Piaractus brachypomus: gross morphology, histology/histochemistry of the mucosal layer and the effects of parasitism by Neoechinorhynchus sp.

Histochemical distribution of four types of enzymes and mucous cells in the gastrointestinal tract of reared half‐smooth tongue sole Cynoglossus semilaevis

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