Histochemical and biochemical characterization of the rat paracervical ganglion

Baker, Harriet; Burke, Jon P.; Bhatnagar, Ranbir K.; Orden, Dianna E. Van; Orden, L. S. Van; Hartman, Boyd K.

doi:10.1016/0006-8993(77)90190-1

Brain Research

1977

DOI: 10.1016/0006-8993(77)90190-1

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Histochemical and biochemical characterization of the rat paracervical ganglion

Harriet Baker

Jon P. Burke

Ranbir K. Bhatnagar

et al.

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1979

2008

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Cited by 17 publications

(2 citation statements)

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“…They may be interneurons, similarly to SIF cells in rat ganglia (Kanerva and Teravainen, 1972;Baker et al, 1977). Gal inhibits noradrenaline release by hypopolarization of the neuronal cell membrane (Reimann and Schneider, 1993;Sevcik et al, 1993), which can be used to moderate the functions of adrenergic neurons in pig PCG.…”

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confidence: 99%

Neurochemical characteristics of paracervical ganglion in the pig

Podlasz¹,

Wąsowicz²

2008

Vet. Med.

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ABSTRACT:A study on the presence of the selected biologically active substances in nerve structures of the paracervical ganglion in the pig was performed with the use of immunofluorescence and RT-PCR. Immunohistochemical methods revealed that 23% of paracervical ganglion (PCG) neurons contain both tyrosine hydroxylase (TH) and dopamine β-hydroxylase (DβH) and that the remaining 77% contain choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT). 73% of TH/DβH neurons contained neuropeptide Y (NPY) and 8% contained somatostatin (Som). All ChAT/VAChT positive neurons contained vasoactive intestinal polypeptide (VIP), 87% of them contained Som, 76% contained NPY and 32% contained neuronal nitric oxide synthase (nNOS). Galanin (Gal) was found only in small cells, which were thought to be SIF cells. No pituitary adenylate cyclase-activating polypeptide (PACAP)-or substance P (SP)-positive neurons were found in PCG. Some areas of PCG contained dense plexuses of ChAT-and VAChT-positive nerve fibres. In the ganglion small number of TH-, nNOS-, NPY-, VIP-, Gal-, PACAP-, Som-and SP-positive nerve fibres was also visible. RT-PCR detected the presence of mRNA for TH, ChAT, nNOS, NPY, VIP, Gal and Som, which were visualised as clearly discernible bands on a gel. In cases of PACAP and SP only weak bands were observed. Keywords: pig; paracervical ganglion; immunohistochemistry; RT-PCRThe paracervical ganglion (PCG) is a unique structure of the autonomic nervous system. It contains components of both the sympathetic and parasympathetic pathways. Postganglionic nerve fibres originating from PCG are responsible for the innervation of the lower urinary and digestive tract as well as reproductive organs.Previous studies (Mitchell, 1993;Keast et al., 1995) demonstrated the presence of two main types of neuronal somata in PCG: cholinergic [containing, choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT)] and adrenergic [containing noradrenaline (NA), tyrosine hydroxylase (TH), dopamine B-hydroxylase (DβH)], so-called "short" adrenergic neurons. Those two populations also contain other substances acting as neurotransmitters, neurotransmitter synthesizing enzyme, neuro-modulators or trophic factors: neuronal nitric oxide synthase (NOS; Majewski et al

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confidence: 99%

Neurochemical characteristics of paracervical ganglion in the pig

Podlasz¹,

Wąsowicz²

2008

Vet. Med.

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“…Acetylcholine (ACh), norepinephrine (NE), neuropeptide Y (NPY), and vasoactive intestinal polypeptide (VIP) have been reported in principal neurons of the PG and in axons innervating the uterine cervix (Shabanah et al, 1964a,b;Adham and Schenk, 1969;Kanerva, 1971;Kanerva 1972a,b;Alm et al, 1980;Allen et al, 1985;Inyama et al, 1985;Papka et al 1987). In addition to principal neurons, the PG also contains small intensely fluorescent (SIF) cells (Kanerva, 1972a;Baker et al, 1977;Papka et al, 1987). The SIF cells of the PG are similar to those described in other autonomic ganglia and contain a primary catecholamine as well as neuropeptides (Dail et al, 1975;Partanen et al, 1980;Papka et al, 1987).…”

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Ontogeny of neurotransmitter systems in the paracervical ganglion and uterine cervix of the rat

Sullivan¹,

Traurig²,

Papka³

1994

Anat. Rec.

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Paracervical ganglia of the female rat: Histochemistry and immunohistochemistry of neurons, SIF cells, and nerve terminals

1987

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The paracervical ganglia of the female rat were studied to elucidate the variety of neural elements in the ganglia. Light and electron microscopy, histochemistry, and immunohistochemistry were employed to reveal subtypes of neurons; small, intensely fluorescent (SIF) cells; and nerve terminals and to examine the relationships between these elements. On the basis of their histochemical markers, four subtypes of principal neurons were identified: acetylcholinesterase (ACHE)-positive, noradrenergic, neuropeptide tyrosine-immunoreactive (NPY-I), and vasoactive intestinal polypeptide-immunoreactive (VIP-I). The NPY-I neurons appeared to be the most numerous and the noradrenergic the least common type of neuron. Four subtypes of chemically coded SIF cells were revealed: catecholamine-containing, NPY-I, and those immunoreactive for calcitonin-gene-related peptide (CGRP-I) and cholecystokinin-octapeptide (CCK-8-I). The SIF cells were present as single cells among and adjacent to principal neurons and as large clusters near the edges of the ganglia or in nearby nerve trunks. Synaptic contacts on SIF cells, or between SIF-cell processes and neurons, were not observed. Seven subtypes of nerve terminals were stained: ACHE-positive, CGRP-I, CCK-8-I, VIP-I, substance P-I, enkephalin-I, and atrial natriuretic factor-I. Nerve terminals enwrapped the neurons as perineuronal plexuses in synaptic-like relationships. These results demonstrate that the paracervical ganglia of the female rat are a complex system of neural elements. For example, several classes of chemically coded neurons, SIF cells, and terminals exist in the ganglia. Each of these components contains a number of substances, some of which are putative neurotransmitters, which could influence activity in the ganglia or in the effector organs innervated by the ganglia.

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Histochemical and biochemical characterization of the rat paracervical ganglion

Cited by 17 publications

References 14 publications

Neurochemical characteristics of paracervical ganglion in the pig

Neurochemical characteristics of paracervical ganglion in the pig

Ontogeny of neurotransmitter systems in the paracervical ganglion and uterine cervix of the rat

Paracervical ganglia of the female rat: Histochemistry and immunohistochemistry of neurons, SIF cells, and nerve terminals

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