Histochemical Analysis of Renal Dysplasia with Ureteral Atresia

Abstract

Unilateral small kidney with ureteral obstruction was discovered in a 74-year-old female cadaver during an anatomical dissection course. In order to elucidate the histogenesis of renal dysplasia, we carried out histochemical and immunohistochemical analyses. On macroscopic view, the kidney was approximately 3 cm in length, 2 cm in width and weighed only 9 g. Although the ureter ran from the renal hilus to the bladder, its width was under 2 mm. The renal parenchyma was extremely thin and there was a large congested vein in the renal sinus. On microscopic examination of the kidney, we observed that numerous developing renal tubules had cytokeratin-positive epithelia, most of which were surrounded by concentric fibrosis. However, we could not detect any structures resembling the collecting duct, renal tubules, renal pelvis, or glomeruli. The concentric mesencymal fibrous tissue surrounding the immature renal tubules contained the smooth muscles that were positive for h-caldesmon. Serial sections of the ureter revealed several small and discontinuous lacunae lined by cuboidal and transitional epithelium, which did not constitute a patent lumen through the bladder. This case is a rare case of renal dysplasia with defect in recanalization of the ureteral bud during the early developmental stage.

Unilateral hydronephrosis and hydroureter secondary to ureteric atresia, and uterus unicornis in a young terrier

“…Young children (Sinha Roy and others 2002) and adults (Bhattacharjee and others 2004) have been diagnosed with ureteric atresia. The condition is accompanied by hydronephrosis, renal hypoplasia or dysplasia (Kawate and others 2009). Decision making in human patients centres around the likelihood of return to function, with severity of renal pelvis dilation a prognostic indicator (Kletscher and others 1991).…”

Expression of cysLT1 and cysLT2 Receptor in Chronic Hyperplastic Eosinophilic Sinusitis

“…For cysLT2R, the specimens were initially stained with H&E, and subsequently photographed. After destaining of the H&E, the sections were incubated with cysLT2R polyclonal antibody (1:20) after pretreatment using an autoclave (20 min at 120°C) [ 12 ]. The immunohistostained samples were photographed again at the same areas on the biopsies.…”

Heat Shock Protein 47: A Novel Biomarker of Phenotypically Altered Collagen-Producing Cells

“…The increased expression and deposition of collagens (type I, type III and type IV) were detected in various organ fibroses in human diseases [ 40 – 42 ], a pattern that is similar to the organ fibrosis observed in experimental models [ 5 , 15 , 72 , 73 ]. Advanced stages of fibrosis compromise the functionality of the involved tissues and lead to the development of complications related to end-stage organ failure [ 1 , 12 , 29 , 38 , 53 , 61 , 68 , 69 ]. For instance, renal fibrosis can alter the water, electrolyte and mineral ion balance, inducing vascular calcification and skeletal mineralization defects [ 27 , 28 , 30 – 33 , 49 ].…”