Histochemical Analyses of Laticifers and Glandular Trichomes in Cannabis sativa

Abstract

The unbranched nonarticulated laticifer, including its latex, and capitate glandular trichomes from Cannabis sativa L. were analyzed in fresh and cryostat preparations with histochemical procedures for the presence of cannabinoids, alkaloids, and other selected cellular components. A positive response to cannabinoid indicators, Duquenois-Negm, fast blue salt B, Gibb, and Beam reagents occurred in laticifers, as well as exuded latex and in disc cells of epidermal capitate glandular trichomes. No response or only an apparent background response to these reagents was detected in other cells. Alkaloids were detected histochemically in laticifers and exuded latex with Wagner, Dittmar, Ellram, chromic acid, Hager, and Dragendorff

Anatomia foliar e caulinar de Chamaecrista trichopoda (Caesalpinioideae) e histoquímica do nectário extrafloral

“…Reagente de Wagner (Furr & Mahlberg, 1981) Amido Lugol (Jensen, 1962) Glicídios Pectinas Vermelho de Rutênio (Johansen, 1940) sendo um nectário extrafloral pedunculado, com cerca de 1 mm de altura (Figura 3A), de coloração alaranjada (Figura 4D, E). Estruturas semelhantes foram relatadas para Cassia obtusifolia (Bhattacharyya & Maheshwari, 1971a).…”

Immunohistochemical localization of caffeine in young Camellia sinensis (L.) O. Kuntze (tea) leaves

“…The alkaloid ancistroheynine A was found in the tip of the shoot and leaf midrib of Liana Ancistrocladus heyneanus and ancistrocladine was found in the branch root of the plant by FT-Raman investigations (Urlaub et al 1997). Many more articles are published describing the localization of different alkaloids with different techniques (Verzár -Petri 1975;Yoder and Mahlberg 1976;Furr and Mahlberg 1981;Neumann et al 1983;Platt and Thomson 1992;Bringmann et al 1996;Mösli Waldhauser and Baumann 1996;Meininger et al 1997;Cai et al 1999;Corsi and Bottega 1999;Bottega et al 2004;Pasqua et al 2004;Alcantara et al 2005;Frosch et al 2006Frosch et al , 2007aNikolakaki and Christodoulakis 2006;Mesjasz -Przybyiowicz et al 2007;Liang et al 2009;Moraes et al 2009;Argyropoulou et al 2010;Christodoulakis et al 2010). …”

Leaf glands of Banisteriopsis muricata (Malpighiaceae): distribution, secretion composition, anatomy and relationship to visitors

“…Methacrylate-embedded samples were also used and sectioned as described above. The following reagents were used to test the secretion: sudan black B (Pearse 1980), sudan IV (Johansen 1940), neutral red (Kirk 1970) and auramine O (Heslop-Harrison 1977) for lipids; Nile blue sulfate (Cain 1947) for acid and neutral lipids; NADI reagent (David & Carde 1964) for essential oils and oleoresins; ferric chloride (Johansen 1940) for total phenolics, Wagner reagent (Furr & Mahlberg 1981) for alkaloids, lugol (Johansen 1940) Images were obtained with a light microscope (AX-70 TRF, Olympus Optical, Tokyo, Japan) coupled to a digital camera (Zeiss AxioCam HRc, Göttinger, Germany) and the Axion Vision image capture program. Fluorochrome analysis and autofluorescence were performed using the same equipment and a epifluorescence system with UV filter (WU: 340-380 nm), dichroic mirror (400 nm) and barrier filter (420 nm).…”