Histoarchitecture of schistosomal granuloma development and involution: morphogenetic and biomechanical approaches

Abstract

The authors present morphogenetic and biomechanical approaches on the concept of the

Collagen arrangement in hepatic granuloma in mice infected with Schistosoma mansoni: dependence on fiber radiation centers

“…Deparaffined sections were desiccated in an incubator at 37 o C for 1 h, and analyzed by scanning electron microscopy (SEM), low-vacuum mode (435-VP, Leo, Cambridge, England). We observed, as previously described (15), that the granuloma stage in the liver is preceded by a lytic pre-granulomatous phase (exudative phase) which produces a spherical space by destruction of the parenchyma for the final establishment and organization of the lesion. In the granulomatous stage, cellular adhesion and sorting play an important role.…”

“…If so, due to a process of minimization of energy, the granuloma would develop in a spherical form, as this is the form in which opposing forces pull equally from all directions and are in balance (15).…”

Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg

“…In previous work, we showed that rabbit polyclonal anti-potato apyrase antibodies did not recognise mammalian NTPDases (FariaPinto et al 2006) and, in this study, similar results were observed. No reactivity was observed in the surrounding granulomatous tissues, which are rich in inflammatory cells such as macrophages, eosinophils, neutrophils, lymphocytes, mast cells and fibroblastic-like cells (Lenzi et al 1998). In addition, the domain from the either mouse NTPDase 5 or NTPDase 6, two intracellular members of the ATP diphosphohydrolase family (Robson et al 2006), has lower identity and similarity with its S. mansoni SmATPDase 2 counterpart.…”

The outcome of acute schistosomiasis infection in adult mice with postnatal exposure to maternal malnutrition

“…Five-micrometre sections were stained with haematoxylin and eosin, Masson's trichrome or Picrosirius for collagen plus polarisation microscopy (Lennert & Parwaresch 1978). Periovular reactions in the liver were classified according to Li Hsü et al (1972) as modified by Lenzi et al (1998). Reactions were classified as the pre-granulomatous stage (weakly reactive or nonreactive and exudative stages) or the granulomatous stage (exudative-productive, productive and involutional granulomas).…”