Histidine-rich glycoprotein polymorphism and pregnancy outcome: a pilot study

Abstract

Histidine-rich glycoprotein (HRG) is involved in fibrinolysis and coagulation, the immune system and angiogenesis. These processes are all crucial in establishing and maintaining pregnancy. The primary aim of this pilot study was to determine if HRG affects pregnancy outcome. The secondary aim was to investigate if a specific genetic polymorphism (rs9898 C/T) in the HRG gene is associated with pregnancy results. The polymorphism leads to expression of either a serine or proline residue at position 186 in the protein sequence. In this study, women undergoing IVF were included. The genetic polymorphism in the HRG gene was analysed by Western blot and single nucleotide polymorphism analysis. None of the women homozygous for the serine at residue 186 became pregnant whereas the women homozygous for proline at residue 186 had higher than expected pregnancy rates. As far as is known,this is the first study to show that a specific genetic polymorphism in the HRG gene of a woman affects her chances of becoming pregnant after IVF. The results may be essential in improving advice and IVF treatment for couples with unexplained infertility.

The histidine-rich glycoprotein A1042G polymorphism and recurrent miscarriage: a pilot study

“…We have previously shown that HRG might be of importance in early pregnancy and this is supported by our prior results indicating an association between the presence of HRG in placenta and risk of preeclampsia during pregnancy [15], as well as between the HRG C633T SNP and pregnancy outcome among patients undergoing in vitro fertilization [18]. …”

“…HRG has been reported to exist as at least ten naturally occurring single nucleotide polymorphisms (SNPs) [16,17]. One of the SNPs, the HRG C633T SNP, has been postulated to be of relevance for implantation and a successful pregnancy [18] and there is an increased occurrence of the homozygous T/T genotype in patients with primary recurrent miscarriage [19]. …”

Histidine‐rich glycoprotein gene polymorphism in patients with recurrent miscarriage

“…The protein has a naturally occurring single nucleotide polymorphism (SNP) in the gene denoted HRG C633T, which results in an amino acid change from proline to serine at position 204 in the protein (in some publications this amino acid is denoted with position 186 in the protein, which describes the protein without its signal peptide). An association between the HRG C633T SNP and pregnancy outcome among patients undertaking in vitro fertilization has been suggested, where women with HRG 633T have a lower than expected pregnancy rate . The aim of this study was to examine the possible association between the HRG 633T SNP and RM.…”

Plasma protein profiling of Mild Cognitive Impairment and Alzheimer’s disease using iTRAQ quantitative proteomics

“…However, the role of CPN in AD is not clear. HRG is involved in fibrinolysis and coagulation [51], and also plays a role in inflammation and immunity [52]. The role of HRG in AD has not been fully investigated, and only one study reported that HRG was decreased in AD sera [53].…”