Histidine kinase two-component response regulators Ssk1, Skn7 and Rim15 differentially control growth, developmental and volatile organic compounds emissions as stress responses in Trichoderma atroviride

Cruz-Magalhães, Valter; Nieto-Jacobo, Marı́a Fernanda; Rostás, Michael; Echaide-Aquino, Jesús Francisco; Naranjo, Ulises Esquivel; Stewart, Alison; Loguercio, Leandro Lopes; Mendoza‐Mendoza, Artemio

doi:10.1016/j.crmicr.2022.100139

Cited by 3 publications

(15 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The VOCs also differed in their concentrations. The fungistatic and fungicidal activities of Trichoderma VOCs, using inverted plate bioassays, have previously been reported [ 27 , 37 ] and were confirmed in the present study. Remarkably, the observed variation in VOC emission is directly associated with the extent of fungistatic activity against the plant pathogen R. solani .…”

Section: Discussionsupporting

confidence: 90%

“…The role of some signaling components in the production of VOCs in T. atroviride has been reported. For example, in the absence of the NADPH Oxidase Nox2 [ 27 ], the Histidine Kinase Two-Component Response Regulator Skn7 [ 37 ] or the MAPK Tmk3 [ 45 ] from T. atroviride IMI206040 were unable to produce 6-pentyl-α-pyrone, the most abundant VOC in this isolate. However, this is not the case in the absence of the NADPH Oxidase Nox1, the NADPH Oxidase Regulator NoxR or the Histidine Kinase Two-Component Response Regulator Ssk1 [ 27 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…For example, in the absence of the NADPH Oxidase Nox2 [ 27 ], the Histidine Kinase Two-Component Response Regulator Skn7 [ 37 ] or the MAPK Tmk3 [ 45 ] from T. atroviride IMI206040 were unable to produce 6-pentyl-α-pyrone, the most abundant VOC in this isolate. However, this is not the case in the absence of the NADPH Oxidase Nox1, the NADPH Oxidase Regulator NoxR or the Histidine Kinase Two-Component Response Regulator Ssk1 [ 27 , 37 ]. The lack of the Histidine Kinase Two-Component Response Regulator Rim15, on the other hand, modifies the accumulation of VOCs in T. atroviride IMI206040 [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, this is not the case in the absence of the NADPH Oxidase Nox1, the NADPH Oxidase Regulator NoxR or the Histidine Kinase Two-Component Response Regulator Ssk1 [ 27 , 37 ]. The lack of the Histidine Kinase Two-Component Response Regulator Rim15, on the other hand, modifies the accumulation of VOCs in T. atroviride IMI206040 [ 37 ]. The role of these signaling components was studied in the interaction with R. solani and Sclerotinia sclerotiorum [ 27 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…The lack of the Histidine Kinase Two-Component Response Regulator Rim15, on the other hand, modifies the accumulation of VOCs in T. atroviride IMI206040 [ 37 ]. The role of these signaling components was studied in the interaction with R. solani and Sclerotinia sclerotiorum [ 27 , 37 ]. These studies illustrated that airborne signals communication is differentially modulated by these signaling components in Trichoderma and the outcome of the interaction is also plant pathogen dependent [ 27 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

Fungistatic Activity Mediated by Volatile Organic Compounds Is Isolate-Dependent in Trichoderma sp. “atroviride B”

Jong

Kandula

Rostás

et al. 2023

JoF

View full text Add to dashboard Cite

Trichoderma spp. produce multiple bioactive volatile organic compounds (VOCs). While the bioactivity of VOCs from different Trichoderma species is well documented, information on intraspecific variation is limited. The fungistatic activity of VOCs emitted by 59 Trichoderma sp. “atroviride B” isolates against the pathogen Rhizoctonia solani was investigated. Eight isolates representing the two extremes of bioactivity against R. solani were also assessed against Alternaria radicina, Fusarium oxysporum f. sp. lycopersici and Sclerotinia sclerotiorum. VOCs profiles of these eight isolates were analyzed using gas chromatography–mass spectrometry (GC-MS) to identify a correlation between specific VOCs and bioactivity, and 11 VOCs were evaluated for bioactivity against the pathogens. Bioactivity against R. solani varied among the fifty-nine isolates, with five being strongly antagonistic. All eight selected isolates inhibited the growth of all four pathogens, with bioactivity being lowest against F. oxysporum f. sp. lycopersici. In total, 32 VOCs were detected, with individual isolates producing between 19 and 28 VOCs. There was a significant direct correlation between VOC number/quantity and bioactivity against R. solani. 6-pentyl-α-pyrone was the most abundant VOC produced, but 15 other VOCs were also correlated with bioactivity. All 11 VOCs tested inhibited R. solani growth, some by >50%. Some of the VOCs also inhibited the growth of the other pathogens by >50%. This study demonstrates significant intraspecific differences in VOC profiles and fungistatic activity supporting the existence of biological diversity within Trichoderma isolates from the same species, a factor in many cases ignored during the development of biological control agents.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Fungistatic Activity Mediated by Volatile Organic Compounds Is Isolate-Dependent in Trichoderma sp. “atroviride B”

Jong

Kandula

Rostás

et al. 2023

JoF

View full text Add to dashboard Cite

show abstract

Selection of redox dye and inoculum conditions for the optimisation of respirometric indices in <i>Verticillium</i> and <i>Trichoderma</i>

Oszust,

Pinzari,

Frąc

2023

Int. Agrophys.

View full text Add to dashboard Cite

A b s t r a c t. Understanding which carbon or nitrogen sources the competing fungal species prefer is pivotal for specific biotechnological applications. However, this is not straightforward, as each strain sometimes behaves differently under the experimental conditions adopted. To analyse the trophic overlap of two species, it is necessary to refine diagnostic techniques and exclude variables that may interfere with the measurements. A protocol for establishing the suitability of chromogenic dyes in the analysis of filamentous fungi with phenotype microarrays is described here. The research goal was to determine the most suitable redox dye indicator and its optimal concentration that reacts quantitatively to the respiratory activity of both Verticillium spp. and Trichoderma spp. isolates in the presence of a nitrogen source. The commercial Biolog TM Redox Dye Mixes D, E, and F and also TTC (2,3,5-Triphenyltetrazolium chloride), INT (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride), and MTT solutions were evaluated in selected concentrations. Both their nutritive and toxic effects were quantified. Results showed that 1% "F" and 0.5% "D" Biolog TM dyes were appropriate for the functional evaluation of isolates belonging to the Verticillium and Trichoderma genera. They can be used in Biolog TM PM-nitrogen plate respiratory assays for a comprehensive functional characterisation of these organisms.K e y w o r d s: redox dyes, formazan, fungi, phenotype microarrays, nitrogen, metabolic profiling

show abstract

Uncovering the multifaceted properties of 6-pentyl-alpha-pyrone for control of plant pathogens

Mendoza-Mendoza,

Esquivel-Naranjo,

Soth

et al. 2024

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Some volatile organic compounds (VOCs) produced by microorganisms have the ability to inhibit the growth and development of plant pathogens, induce the activation of plant defenses, and promote plant growth. Among them, 6-pentyl-alpha-pyrone (6-PP), a ketone produced by Trichoderma fungi, has emerged as a focal point of interest. 6-PP has been isolated and characterized from thirteen Trichoderma species and is the main VOC produced, often accounting for >50% of the total VOCs emitted. This review examines abiotic and biotic interactions regulating the production of 6-PP by Trichoderma, and the known effects of 6-PP on plant pathogens through direct and indirect mechanisms including induced systemic resistance. While there are many reports of 6-PP activity against plant pathogens, the vast majority have been from laboratory studies involving only 6-PP and the pathogen, rather than glasshouse or field studies including a host plant in the system. Biopesticides based on 6-PP may well provide an eco-friendly, sustainable management tool for future agricultural production. However, before this can happen, challenges including demonstrating disease control efficacy in the field, developing efficient delivery systems, and determining cost-effective application rates must be overcome before 6-PP’s potential for pathogen control can be turned into reality.

show abstract

Histidine kinase two-component response regulators Ssk1, Skn7 and Rim15 differentially control growth, developmental and volatile organic compounds emissions as stress responses in Trichoderma atroviride

Cited by 3 publications

References 66 publications

Fungistatic Activity Mediated by Volatile Organic Compounds Is Isolate-Dependent in Trichoderma sp. “atroviride B”

Fungistatic Activity Mediated by Volatile Organic Compounds Is Isolate-Dependent in Trichoderma sp. “atroviride B”

Selection of redox dye and inoculum conditions for the optimisation of respirometric indices in <i>Verticillium</i> and <i>Trichoderma</i>

Uncovering the multifaceted properties of 6-pentyl-alpha-pyrone for control of plant pathogens

Contact Info

Product

Resources

About