Histidine Hydrogen Exchange for Analysis of Protein Folding, Structure, and Function

Fitzgerald, Michael C.; Jin, Lorrain; Tran, Duc

doi:10.1002/9781118703748.ch10

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Hdx Rates In Various Functional Groups1

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2020

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“…The second half of the exchange reaction is a deuteron transfer from the bulk D 2 O to imidazole anion. Although the proton at C2 position of imidazole group may also exchange with deuteron in the solvent at a significantly slower rate, , the reaction is not discussed here because it is outside of the scope of this manuscript. …”

Section: Hdx Rates In Various Functional Groupsmentioning

confidence: 99%

Tutorial: Chemistry of Hydrogen/Deuterium Exchange Mass Spectrometry

Hamuro¹

2020

J. Am. Soc. Mass Spectrom.

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Chemistry related to hydrogen/deuterium exchange–mass spectrometry (HDX–MS) for the analysis of proteins is described. First, the HDX rates of various functional groups in proteins are explained by reviewing the observed rates described in the literature, followed by estimating rates of all types of heteroatom hydrogens in proteins using proton transfer theory and the pK a values. The estimated HDX rates match well with the respective observed rates for most functional groups, with the exception of indole and amide groups. The discrepancies between the observed and estimated HDX rates for these groups are explained by the reaction mechanisms. Second, the factors that affect the HDX rates of backbone amide hydrogen, including side chain, N- and C-terminals, pH, temperature, organic solvent, and isotopes, are discussed. These factors are important for the proper design of exchange reactions and downstream process as well as the analysis and interpretation of HDX–MS data.

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Section: Hdx Rates In Various Functional Groupsmentioning

confidence: 99%

Tutorial: Chemistry of Hydrogen/Deuterium Exchange Mass Spectrometry

Hamuro¹

2020

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

Histidine Hydrogen Exchange for Analysis of Protein Folding, Structure, and Function

Cited by 1 publication

References 38 publications

Tutorial: Chemistry of Hydrogen/Deuterium Exchange Mass Spectrometry

Tutorial: Chemistry of Hydrogen/Deuterium Exchange Mass Spectrometry

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