Histidine-conjugated DNA as a biomolecular depot for metal ions

Park, So‐Young; Matsui, Hotaka; Fukumoto, Koyuki; Yum, Ji Hye; Sugiyama, Hiroshi

doi:10.1039/d0ra01267j

Cited by 11 publications

(12 citation statements)

References 63 publications

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“…To exploit this advantage in organic syntheses, we have investigated the catalytic functions of DNA with unnatural monomer units. For example, we have incorporated a bipyridine ligand into DNA to generate DNA metalloenzymes for enantioselective hydration reactions and devised histidine-modified oligonucleotides showing remarkable metal-binding ability and catalytic performance for DNA-based asymmetric catalysis as well as ABTS oxidation reactions. − This “bottom-up” molecular engineering technology is useful in endowing DNA/RNA oligonucleotides with new functions such as catalysis and molecular switching.…”

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confidence: 99%

Photo-Controllable Phase Transition of Arylazopyrazole-Conjugated Oligonucleotides

et al. 2021

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Phase transition is a promising aspect of DNA as biopolymers. Anionic DNA oligonucleotides easily form complexes with cationic polypeptides such as polylysine, and duplex formation significantly influences their complexation and resulting microcompartments. In this study, phase transition of microcompartments containing DNA and polylysine was systematically induced by modulating duplex formation of arylazopyrazoleconjugated oligonucleotides with light. We demonstrated that UV irradiation destabilized DNA duplex and generated isotropic coacervates, while duplex stabilization by visible light irradiation caused the formation of liquid crystalline coacervates. This photocontrol of phase transition was highly repeatable, and similar changes were observed even after ten cycles of light irradiation. Our approach would provide a robust control layer to the development of tailor-made microcompartments.

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confidence: 99%

Photo-Controllable Phase Transition of Arylazopyrazole-Conjugated Oligonucleotides

et al. 2021

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“…The structure and function of G‐quadruplexes have been extensively studied to reveal the regulatory effect of G‐quadruplexes. Modified oligonucleotides have been exploited to manipulate the topology and stability of G‐quadruplexes [43–46] . In this study, we selected human‐telomere sequence called 22AG, 5’‐AG 3 (TTAG 3 ) 3 ‐3’.…”

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confidence: 99%

“…Modified oligonucleotides have been exploited to manipulate the topology and stability of G-quadruplexes. [43][44][45][46] In this study, we selected humantelomere sequence called 22AG, 5'-AG 3 (TTAG 3 ) 3 -3'. 2FA was incorporated into 7, 13 and 19 position in place of adenine on loop region.…”

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Characterization of 2‐Fluoro‐2’‐deoxyadenosine in Duplex, G‐Quadruplex and i‐Motif

2022

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Among various kinds of fluorine‐substituted biomolecules, 2‐fluoroadenine (2FA) and its derivatives have been actively investigated as therapeutic reagents, radio‐sensitizers, and 19F NMR probes. In spite of their excellent properties, DNA containing 2FA has not been studied well. For fundamental understanding and future applications to the development of functional nucleic acids, we characterized 2FA‐containing oligonucleotides for canonical right‐handed DNA duplex, G‐quadruplex, and i‐motif structures. Properties of 2FA were similar to native adenine due to the small size of the fluorine atom, but it showed unique features caused by high electronegativity. This work provides useful information for future application of 2FA‐modified DNA.

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“…In a recent study, Park et al . have devised and reported amino acid‐imbedded DNA metalloenzymes [104] . They have established a systematic modular strategy for introducing functional moieties utilizing well‐explored d ‐threoninol scaffolds.…”

Section: Dna‐based Hybrid Catalystsmentioning

confidence: 99%

Harnessing DNA as a Designable Scaffold for Asymmetric Catalysis: Recent Advances and Future Perspectives

Yum

Sugiyama

Park

2022

The Chemical Record

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Since the first report of DNAzyme by in vitro selection in 1994, catalytic DNA has investigated extensively, and their application has expanded continually in virtue of rapid advances in molecular biology and biotechnology. Nowadays, DNA is in the second prime time by way of DNA-based hybrid catalysts and DNA metalloenzymes in which helical chirality of DNA serves to asymmetric catalysis. DNA-based hybrid catalysts are attractive system to respond the demand of the times to pursuit green and sustainable society beyond traditional catalytic systems that value reaction efficiency. Herein, we highlight the recent advances and perspective of DNA-based hybrid catalysts with various aspects of DNA as a versatile scaffold for asymmetric synthesis. We hope that scientists in a variety of fields will be encouraged to join and promote remarkable evolution of this interesting research.

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Histidine-conjugated DNA as a biomolecular depot for metal ions

Abstract: Histidine is a versatile amino acid residue that plays a critical role in the active sites of many metalloenzymes.

Cited by 11 publications

References 63 publications

Photo-Controllable Phase Transition of Arylazopyrazole-Conjugated Oligonucleotides

Photo-Controllable Phase Transition of Arylazopyrazole-Conjugated Oligonucleotides

Characterization of 2‐Fluoro‐2’‐deoxyadenosine in Duplex, G‐Quadruplex and i‐Motif

Harnessing DNA as a Designable Scaffold for Asymmetric Catalysis: Recent Advances and Future Perspectives

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