Histidine-based coordinative polymers for efficient intracellular protein delivery <i>via</i> enhanced protein binding, cellular uptake, and endosomal escape

Chen, Changyuan; Gao, Peng; Wang, Hui; Cheng, Yiyun; Lv, Jia

doi:10.1039/d2bm01541b

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…The imidazole groups of histidine and guanidine groups in arginine enhance the cellular uptake of material and undergo protonation in endosomes. This promotes the destabilization of endosomal membranes and escapes via the proton sponge effect [ 56 , 70 , 71 ]. Functionalization of carrier molecules with histidine and arginine provides a possibility of enhanced therapeutic delivery which influences the destabilization of endosomal membranes [ 72 , 73 ].…”

Section: Resultsmentioning

confidence: 99%

Amino Acid-Coated Zeolitic Imidazolate Framework for Delivery of Genetic Material in Prostate Cancer Cell

Polash,

Garlick-Trease,

Pyreddy

et al. 2023

Molecules

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Metal–organic frameworks (MOFs) are currently under progressive development as a tool for non-viral biomolecule delivery. Biomolecules such as proteins, lipids, carbohydrates, and nucleic acids can be encapsulated in MOFs for therapeutic purposes. The favorable physicochemical properties of MOFs make them an attractive choice for delivering a wide range of biomolecules including nucleic acids. Herein, a green fluorescence protein (GFP)-expressing plasmid DNA (pDNA) is used as a representative of a biomolecule to encapsulate within a Zn-based metal–organic framework (MOF) called a zeolitic imidazolate framework (ZIF). The synthesized biocomposites are coated with positively charged amino acids (AA) to understand the effect of surface functionalization on the delivery of pDNA to prostate cancer (PC-3) cells. FTIR and zeta potential confirm the successful preparation of positively charged amino acid-functionalized derivatives of pDNA@ZIF (i.e., pDNA@ZIFAA). Moreover, XRD and SEM data show that the functionalized derivates retain the pristine crystallinity and morphology of pDNA@ZIF. The coated biocomposites provide enhanced uptake of genetic material by PC-3 human prostate cancer cells. The AA-modulated fine-tuning of the surface charge of biocomposites results in better interaction with the cell membrane and enhances cellular uptake. These results suggest that pDNA@ZIFAA can be a promising alternative tool for non-viral gene delivery.

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Section: Resultsmentioning

confidence: 99%

Amino Acid-Coated Zeolitic Imidazolate Framework for Delivery of Genetic Material in Prostate Cancer Cell

Polash,

Garlick-Trease,

Pyreddy

et al. 2023

Molecules

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“…A dose-dependent decrease in cell viability was observed with delivery of cytotoxic proteins: saporin, RNase A, and α-chymotrypsin, indicating bioactivity following delivery. Recently, native protein delivery was also demonstrated by Zn 2+ , Ni 2+ , and Cu 2+ immobilized dendrimers [ 194 ].…”

Section: Methods For Intracellular Protein Deliverymentioning

confidence: 99%

Intracellular Protein Delivery: Approaches, Challenges, and Clinical Applications

Chan,

Tsourkas

2024

BME Front

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Protein biologics are powerful therapeutic agents with diverse inhibitory and enzymatic functions. However, their clinical use has been limited to extracellular applications due to their inability to cross plasma membranes. Overcoming this physiological barrier would unlock the potential of protein drugs for the treatment of many intractable diseases. In this review, we highlight progress made toward achieving cytosolic delivery of recombinant proteins. We start by first considering intracellular protein delivery as a drug modality compared to existing Food and Drug Administration-approved drug modalities. Then, we summarize strategies that have been reported to achieve protein internalization. These techniques can be broadly classified into 3 categories: physical methods, direct protein engineering, and nanocarrier-mediated delivery. Finally, we highlight existing challenges for cytosolic protein delivery and offer an outlook for future advances.

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Protein@PP-Zn nanocomplex assembled by coordination of zinc ions used for intracellular protein delivery

Cui

Liu

et al. 2023

Sci. China Chem.

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Histidine-based coordinative polymers for efficient intracellular protein delivery via enhanced protein binding, cellular uptake, and endosomal escape

Abstract: Polymers are one of the most promising protein delivery carriers, however their applications are hindered by low delivery efficacy owning to undesirable performance in protein binding, cellular uptake and endosomal...

Cited by 4 publications

References 47 publications

Amino Acid-Coated Zeolitic Imidazolate Framework for Delivery of Genetic Material in Prostate Cancer Cell

Amino Acid-Coated Zeolitic Imidazolate Framework for Delivery of Genetic Material in Prostate Cancer Cell

Intracellular Protein Delivery: Approaches, Challenges, and Clinical Applications

Protein@PP-Zn nanocomplex assembled by coordination of zinc ions used for intracellular protein delivery

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