Histamine Release Factor from Dermanyssus gallinae (De Geer): Characterization and in vitro assessment as a protective antigen

Bartley, Kathryn; Nisbet, Alasdair J.; Offer, J. E.; Sparks, Nicholas; Wright, Harry W.; Huntley, John F.

doi:10.1016/j.ijpara.2008.09.006

Cited by 28 publications

(40 citation statements)

References 54 publications

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“…In the case of D. gallinae, however, the natural immune response to exposed antigens appears ineffective in controlling mite populations in poultry houses. A previous study has shown that there was no correlation between mite populations and IgY antibody titres in egg yolk (Arkle et al 2006) and Western blots of D. gallinae soluble protein fraction probed with IgY extracted from egg yolks collected from a commercial farm infested with mites did not show substantial detection of any antigens (Bartley et al 2008).…”

Section: Introductionmentioning

confidence: 76%

The testing of antibodies raised against poultry red mite antigens in an in vitro feeding assay; preliminary screen for vaccine candidates

et al. 2009

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Dermanyssus gallinae (De Geer), the poultry red mite, is a blood-feeding ectoparasite that infests many bird species. We have used an in vitro feeding assay to allow the identification of protective D. gallinae antigens that may have potential as vaccine candidates. Homogenised mites were extracted sequentially with PBS, Tween 20, Triton X100 and urea giving four protein fractions. Five experimental groups of Lohmann Brown hens were used to generate antibodies; four groups were injected with one of each of the protein fractions in QuilA adjuvant and a control group was injected with adjuvant only. Booster injections were administered 2 and 4 weeks after initial immunisation. Eggs were collected throughout the experiment and soluble IgY antibodies were extracted from a pool of egg yolks collected at week six post-injection. Western blots, performed using post vaccination antibodies from test and control groups, revealed a strong antibody response against a range of injected proteins. Fresh chicken blood, supplemented with antibodies raised against these protein fractions, was fed to mites in an in vitro feeding assay in order to determine whether the antibodies had an anti-mite effect. Although there was variability in the numbers of feeding mites, it was found that the strongest anti-mite effect was seen with the PBS protein fraction, which had a cumulative average mortality of 34.8% 14 days after feeding compared with 27.3% for the control group (P = 0.043).

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Section: Introductionmentioning

confidence: 76%

The testing of antibodies raised against poultry red mite antigens in an in vitro feeding assay; preliminary screen for vaccine candidates

et al. 2009

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“…Immunizing hosts with gut-derived concealed antigens has proven successful for development of the vaccine TickGARD® (Intervet, Bedingo, Australia) against the midgut-expressed BM86 protein of the cattle tick Rhipicephalus microplus (Willadsen et al, 1995). Although no homologue to BM86 has been found in PRM, the same strategy has been pursued recently using other internally expressed proteins Bartley et al, 2009;Bartley et al, 2012).…”

Section: Digestive Tractmentioning

confidence: 99%

Understanding the biology and control of the poultry red miteDermanyssus gallinae: a review

et al. 2015

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Dermanyssus gallinae, the poultry red mite (PRM), is a blood-feeding ectoparasite capable of causing pathology in birds, amongst other animals. It is an increasingly important pathogen in egg layers and is responsible for substantial economic losses to the poultry industry worldwide. Even though PRM poses a serious problem, very little is known about the basic biology of the mite. Here we review the current body of literature describing red mite biology and discuss how this has been, or could be, used to develop methods to control PRM infestations. We focus primarily on the PRM digestive system, salivary glands, nervous system and exoskeleton and also explore areas of PRM biology which have to date received little or no study but have the potential to offer new control targets.

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“…Bartley et al (2009) identified an orthologue of tick histamine release factor (HRF) in D. gallinae and suggested the protein could have a regulatory function in mites. Following production of the recombinant protein Dg-HRF-1, in vitro testing against D. gallinae demonstrated a significant 7% increase in mite mortality compared to controls.…”

Section: Vaccinesmentioning

confidence: 99%

Opportunities for integrated pest management to control the poultry red mite, Dermanyssus gallinae

Harrington

George

Guy

et al. 2011

World's Poultry Science Journal

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Dermanyssus gallinae is the most economically important ectoparasite of laying hens in Europe. Control of D. gallinae is already hampered by issues of pesticide resistance and product withdrawal. With the prohibition of conventional cages in 2012 and the resulting switch to more structurally complex housing which favours red mite, the importance of managing this pest is expected to increase. Integrated Pest Management (IPM), as often employed in agricultural pest control, may be a way to address these issues where a combination of different novel control methods could be used with/without conventional management techniques to provide a synergistic and more efficacious effect. Work at in our laboratory has shown that essential oils, including thyme and garlic, may act as effective D. gallinae repellents and acaricides, whilst preliminary vaccine studies have demonstrated a significant increase in mite mortality in vitro using concealed antigens. Work elsewhere has considered predators and fungi for D. gallinae control and other husbandry techniques such as manipulating temperature and lighting regimes in poultry units. This paper will review the available and emerging techniques for D. gallinae control and discuss which techniques might be suitable for inclusion in an integrated management programme (e.g. synthetic acaricides and diatomaceous earths).

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Histamine Release Factor from Dermanyssus gallinae (De Geer): Characterization and in vitro assessment as a protective antigen

Cited by 28 publications

References 54 publications

The testing of antibodies raised against poultry red mite antigens in an in vitro feeding assay; preliminary screen for vaccine candidates

The testing of antibodies raised against poultry red mite antigens in an in vitro feeding assay; preliminary screen for vaccine candidates

Understanding the biology and control of the poultry red miteDermanyssus gallinae: a review

Opportunities for integrated pest management to control the poultry red mite, Dermanyssus gallinae

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