Histamine release by vagal stimulation

Abstract

The content of acetylcholine and histamine in the effluent of isolated, vagally innerved guinea-pig auricles was determined. Spontaneous or stimulation-induced overflow of acetylcholine was detected only in the presence of acetylcholinesterase inhibitors. The histamine overflow was measurable also in the absence of inhibition of cholinesterase, and neatly increased during vagal stimulation. The vagally evoked histamine overflow was blocked by atropine and potentiated by eserine. The stimulation-induced histamine overflow in the effluent is discussed, presumably assuming that acetylcholine may release histamine from cardiac histamine stores.

Mediator release from isolated rat ileum in response to field stimulation

“…The experiments reported here are in keeping with our previous observations on cholinergic histamine release: acetylcholine causes histamine secretion in isolated rat peritoneal mast cells and vagal stimulation produces a net increase in acetylcholine and histamine overflows in isolated, vagally innervated guinea-pig auricles [14][15][16][17].…”

“…Thus, the link between cholinergic system and histamine stores, firmly established by a variety of experimental evidence [15,16] is reinforced by the present studies showing that parasympathetic stimulation releases acetylcholine which in turn triggers histamine secretion, presumably from intestinal mast cells.…”

“…Histamine secretion from mast cells occur in the presence of nanomolar concentrations of acetylcholine; atropine competitively blocks the acetylcholine-induced histamine secretion, indicating the presence of cholinergic muscarinic receptors on mast cells, which have been also identified by means of 3H-QNB binding [15,16]. Acetylcholine, added to perfusates recirculating at a constant rate through isolated guinea-pig heart, significantly increased the amount of histamine appearing in the perfusates [17]; in isolated, vagaUy innervated guinea-pig auricles, the histamine overflow neatly increased during vagal stimulation, in a way which was blocked by atropine and potentiated by eserine [16]. The present observations that exogenous acetylcholine evokes histamine release from rat ileum is in keeping with our previous results.…”

The release of histamine by parasympathetic stimulation in guinea‐pig auricle and rat ileum.

“…We have selected these preparations from different animal species containing connective tissue mast cells and mucosal mast cells, in consideration of mast cell heterogeneity in response to the same stimulus (Bienenstock, Befus, Pearce, Denburg & Goodacre, 1982). A preliminary report of some of the results was communicated at the European Histamine Research Society Meetings (Blandina, Barattini, Fantozzi, Masini & Mannaioni, 1983;Blandina, Barattini, Fantozzi, Masini, Brunelleschi & Mannaioni, 1984).…”

Synergistic interaction between histamine and ouabain on ventricular fibrillation threshold

“…Because of this synergy, extremely low concentrations ofhistamine might greatly increase the cardiotoxic effects of ouabain. Histamine is present in the heart and can be released by antigenantibody reactions (Feigen & Prager, 1969;Levi, 1972), drugs (Lorenz, 1975;Levi et al, 1982), and nerve stimulation (Blandina et al, 1983;Gross et al, 1984) in quantities sufficient to interact with cardiac glycosides. Further studies are required to assess the clinical significance of this synergistic interaction.…”