Histamine-induced villous damage in the rat duodenum.

Tanaka, Hironori; Takeuchi, Koji; Okabe, Susumu

doi:10.1254/jjp.51.291

Cited by 7 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is surprising since the gut is frequently in contact with this amine, owing to the fact that it is present in many foods (1,2). The few studies on the effects of histamine on intestinal mucosa were done in animals (7,8), and the route of administration used in those studies did not correspond to the physiologic conditions of histamine ingestion. Nevertheless, the results demonstrate that histamine can modify intestinal architecture.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the results demonstrate that histamine can modify intestinal architecture. Tanaka et al demonstrated that a single subcutaneous dose of histamine (20 mg/kg) in rats induced villous changes in the duodenum, consisting of severe exfoliation of epithelial cells and exposure of the lamina propria (8). These changes were reversed within 8 h, indicating rapid mucosal repair.…”

Section: Discussionmentioning

confidence: 99%

“…Little is known about the direct effects of ingested histamine on the intestinal mucosa. Studies in animals have demonstrated that histamine administered by infusion into mesenteric blood vessels damages the duodenal epithelium (7,8).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Ultrastructural changes in the duodenal mucosa induced by ingested histamine in patients with chronic urticaria

Kanny

Grignon²,

Dauça

et al. 1996

Allergy

View full text Add to dashboard Cite

Histamine in food may be responsible for some cases of food intolerance. We previously demonstrated disturbances in the metabolism of ingested histamine in patients with chronic urticaria (CU) and proposed that this could be related to increased intestinal permeability to histamine. The present study was undertaken to look for ultrastructural changes in the intestinal tract that might explain this abnormality. We examined duodenal biopsies from seven patients with CU before and after intraduodenal administration of histamine (120 mg). Five subjects had clinical symptoms (diarrhea, urticaria, headache, accelerated heart rate, and drop in blood pressure) within 1 h of duodenal histamine challenge (DHC). Ultrastructural changes, including edema of the interstitial tissue, enlargement of the basal intercellular spaces, slight congestion of the endothelial cells, and pericapillary edema, were observed in six subjects 45 min after DHC. In all the biopsies, the epithelium was normal, and the tight junctions were not modified by DHC. This morphologic study demonstrates that histamine can induce edema in the basal intercellular spaces of the duodenal mucosa and in the submucosa without evident change in the integrity of intercellular junctions. The most plausible route for histamine to have taken would appear to be an intracellular one.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrastructural changes in the duodenal mucosa induced by ingested histamine in patients with chronic urticaria

Kanny

Grignon²,

Dauça

et al. 1996

Allergy

View full text Add to dashboard Cite

show abstract

“…Two specimens per animal were analysed by an observer unaware of the experimental procedures. The examined intestinal surface was scored into four categories depending on the degree of mucosal changes, according to a modified scale proposed by Tanaka et al . (1989).…”

Section: Methodsmentioning

confidence: 99%

Effects of hypovolaemia on acid‐induced duodenal mucosal damage in the rat

Casselbrant

Holm

Bothmer

et al. 2001

Acta Physiologica Scandinavica

View full text Add to dashboard Cite

Modest acute hypovolaemia in rats markedly decreases the duodenal mucosal alkaline secretion via neurohumoral links. The present study was undertaken to investigate if such a procedure influences the morphological changes observed following an acid challenge of the duodenal mucosa. Experiments were performed on anaesthetized male Sprague-Dawley rats. HCl (10 or 100 mM) was infused during 15 min into the duodenum via a luminally situated catheter. Time controls were compared with animals bled 10% of total blood volume. Mucosal damage was evaluated by light microscopic morphometry on transverse sections and by scanning electron microscopy of the luminal surface. Perfusion with either 10 mM or 100 mM HCl reduced villus length by about 30%. The villus area was unaffected by 10 mM HCl, but was reduced significantly by 10% by 100 mM HCl as compared with NaCl time controls. Hypovolaemia did not influence the morphometrical changes induced by 10 mM HCl but reduced significantly both villus length (-28%) and villus area (-10%) as compared with the unbled 100 mM HCl group. Scanning electrone microscopy (SEM)-based visual damage score was not influenced by the hypovolaemia procedure in any of the acidities. Morphological changes of the duodenal mucosa, induced by moderate intra-luminal acidity (10 mM HCl), is not influenced by hypovolaemia. However, at higher acidities (100 mM HCl) the hypovolaemia contributes to more severe mucosal damage.

show abstract