Histamine increases sphingosine kinase-1 expression and activity in the human arterial endothelial cell line EA.hy 926 by a PKC-α-dependent mechanism

Huwiler, Andrea; Döll, Frauke; Ren, Shen; Klawitter, Sabine; Greening, Anna; Römer, Isolde; Bubnova, Svetlana; Reinsberg, Luise; Pfeilschifter, Josef

doi:10.1016/j.bbalip.2006.02.007

Cited by 55 publications

(63 citation statements)

References 42 publications

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“…To see whether the second delayed phase of SK activation depends on increased SK-1 protein expression, Western blot analyses were performed, using a specific antibody against SK-1 which was previously characterized (Döll et al 2005, Huwiler et al 2006.…”

Section: Resultsmentioning

confidence: 99%

“…Cell culturing and siRNA transfections MCF7 cells were cultured as previously reported (Döll et al 2005), and gene silencing of SKs was conducted exactly as described (Huwiler et al 2006).…”

Section: Chemicalsmentioning

confidence: 99%

Section: Promoter Studiesmentioning

confidence: 99%

“…To measure SK-1 promoter activity, a luciferase reporter gene assay was performed exactly as previously described (Huwiler et al 2006) using a Lumat LB9507 luminometer (Berthold Detection Systems, Pforzheim, Germany). SK activity assay SK-1 activity was measured exactly as previously described (Döll et al 2005, Huwiler et al 2006. In brief, 30 mg protein extracts and 10 ml of 1 mM sphingosine (dissolved in 1 mg/ml BSA) were mixed with SK-1 buffer (20 mM Tris, pH 7.4, 20% glycerol, 1 mM mercaptoethanol, 1 mM EDTA, 1 mM sodium orthovanadate, 40 mM b-glycerophosphate, 15 mM NaF, 10 mg/ml leupeptin, 10 mg/ml aprotinin, 10 mg/ ml soybean trypsin inhibitor, 1 mM phenylmethylsulfonyl fluoride) in a total volume of 190 ml.…”

Section: Promoter Studiesmentioning

confidence: 99%

“…Although S1P may exert its effects via both intracellular and extracellular actions and targets, most of the so-far-described cellular responses triggered by S1P are mediated through specific cell surface S1P receptors, the S1P 1-5 receptors, which belong to the class of G-protein-coupled receptors (Hla 2003). S1P is produced by sphingosine kinases (SKs) which can be activated by a variety of stimuli that couple either to receptor tyrosine kinases like the platelet-derived and epidermal growth factors (EGFs) or to various G-proteincoupled receptors (Alemany et al 2000, Huwiler et al 2006. To date, two SK subtypes have been cloned and partially characterized, SK-1 and SK-2 (Kohama et al 1998, Liu et al 2000.…”

Section: Introductionmentioning

confidence: 99%

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Prolactin upregulates sphingosine kinase-1 expression and activity in the human breast cancer cell line MCF7 and triggers enhanced proliferation and migration

Döll¹,

Pfeilschifter²,

Huwiler³

2007

Endocr Relat Cancer

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Sphingosine kinases (SK) catalyze the formation of sphingosine-1-phosphate (S1P) which plays a crucial role in cell growth and survival. Here, we show that prolactin (PRL) biphasically activates the SK-1, but not the SK-2 subtype, in the breast adenocarcinoma cell-line MCF7. A first peak occurs after minutes of stimulation and is followed by a second delayed activation after hours of stimulation. A similar biphasic effect on SK-1 activity is seen for 17b-estradiol (E 2 ). The delayed activation of SK-1 derives from an upregulated mRNA and protein expression and is due to increased SK-1 promoter activity and mechanistically involves STAT5 activation as well as protein kinase C and the classical mitogen-activated protein kinases. Furthermore, glucocorticoids also block both hormone-induced SK-1 expression and activity. Functionally, long-term stimulation of MCF7 cells with PRL or E 2 is well known to trigger increased cell proliferation and migration. Both hormone-induced cell responses critically involve SK-1 activation since the depletion of SK-1, but not SK-2, by siRNA transfection abolishes the hormone-induced cell proliferation and migration. In summary, our data show that PRL and E 2 cause a pronounced delayed SK-1 activation which is due to increased gene transcription, and critically determines the capability of cells to grow and move. Thus, the SK-1 may represent a novel attractive target for anti-tumor therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Chemicalsmentioning

confidence: 99%

Section: Promoter Studiesmentioning

confidence: 99%

Section: Promoter Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Prolactin upregulates sphingosine kinase-1 expression and activity in the human breast cancer cell line MCF7 and triggers enhanced proliferation and migration

Döll¹,

Pfeilschifter²,

Huwiler³

2007

Endocr Relat Cancer

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Sphingosine Kinases: Biochemistry, Regulation, and Roles

Pitman

Jarman

Leclercq

et al. 2013

Lysophospholipid Receptors

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Involvement of calcium in 50‐Hz magnetic field‐induced activation of sphingosine kinase 1 signaling pathway

Yang

Ye²,

Chen

et al. 2019

Bioelectromagnetics

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Previously, we found that exposure to a 50-Hz magnetic field (MF) could induce human amniotic epithelial (FL) cell proliferation and sphingosine kinase 1 (SK1) activation, but the mechanism was not clearly understood. In the present study, the possible signaling pathways which were involved in SK1 activation induced by 50-Hz MF exposure were investigated. Results showed that MF exposure increased intracellular Ca 2þ which was dependent on the L-type calcium channel, and induced Ca 2þdependent phosphorylation of extracellular regulated protein kinase (ERK), SK1, and protein kinase C a (PKCa). Also, treatment with U0126, an inhibitor of ERK, could block MF-induced SK1 phosphorylation, but had no effect on PKCa phosphorylation. Also, the inhibitor of PKCa, G€ o6976, had no effect on MF-induced SK1 activation in FL cells. In addition, the activation of ERK and PKCa could be abolished by SKI II, the inhibitor of SK1. In conclusion, the intracellular Ca 2þ mediated the 50-Hz MF-induced SK1 activation which enhanced PKCa phosphorylation, and there might be a feedback mechanism between SK1 and ERK activation in responding to MF exposure in FL cells.

show abstract

Histamine increases sphingosine kinase-1 expression and activity in the human arterial endothelial cell line EA.hy 926 by a PKC-α-dependent mechanism

Cited by 55 publications

References 42 publications

Prolactin upregulates sphingosine kinase-1 expression and activity in the human breast cancer cell line MCF7 and triggers enhanced proliferation and migration

Prolactin upregulates sphingosine kinase-1 expression and activity in the human breast cancer cell line MCF7 and triggers enhanced proliferation and migration

Sphingosine Kinases: Biochemistry, Regulation, and Roles

Involvement of calcium in 50‐Hz magnetic field‐induced activation of sphingosine kinase 1 signaling pathway

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