Histamine Downregulates the Th1-Associated Chemokine IP-10 in Monocytes and Myeloid Dendritic Cells

Glatzer, Franziska; Mommert, Susanne; Köther, Brigitta; Gschwandtner, Maria; Stark, Holger; Werfel, Thomas; Gutzmer, Ralf

doi:10.1159/000355960

Cited by 19 publications

(21 citation statements)

References 38 publications

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“…Histamine downregulated the production of CCL2 in human monocytes, Langerhans cells, inflammatory dendritic epidermal cells, and slan-dendritic cells (Dijkstra et al, 2007Gschwandtner et al, 2011Gschwandtner et al, , 2012. Furthermore, other Th1-promoting cytokines and chemokines were downregulated in a variety of human antigenpresenting cells via H 4 receptor stimulation, such as downregulation of TNFa, interferon a, and CXCL8 on plasmacytoid dendritic cells , and IP-10 on monocytes and myeloid dendritic cells (Glatzer et al, 2014). Stimulation of the H 4 receptor Histamine Receptors induced IL-31 production in peripheral blood mononuclear cells (PBMC) or Th2 polarized T cells, and this production was significantly increased in PBMC from subjects with atopic dermatitis compared with healthy controls .…”

Section: Functionmentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. XCVIII. Histamine Receptors

et al. 2015

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Section: Functionmentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. XCVIII. Histamine Receptors

et al. 2015

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“…Nevertheless, the implication of CKR in nonallergic asthma remains to be established. We therefore studied the expression of Th1-typical CKR such as CCR2 [7], CCR5 [4], CCR6 [5,8], and CXCR4 [4,9], as well as CXCR3 (Th2- and Th1-typical CKR) [4,10,11,12,13], in circulating T lymphocytes from nonallergic asthma patients.…”

Section: Introductionmentioning

confidence: 99%

Abnormal Chemokine Receptor Profile on Circulating T Lymphocytes from Nonallergic Asthma Patients

Barbarroja-Escudero

Prieto-Martín

Monserrat-Sanz

et al. 2014

Int Arch Allergy Immunol

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Background: T lymphocytes are involved in the pathogenesis of nonallergic asthma. The objective of this study was to characterize the subset distribution and pattern of chemokine receptor expression in circulating T lymphocyte subsets from nonallergic asthma patients. Methods: Forty stable nonallergic asthma patients and 16 sex- and age-matched healthy donors were studied. Twelve patients did not receive inhaled steroids (untreated patients), 16 received 50-500 μg b.i.d. of inhaled fluticasone propionate (FP) (standard-dose patients), and 12 received over 500 μg b.i.d. of inhaled FP (high-dose patients) for at least 12 months prior to the beginning of this study and were clinically well controlled. Flow cytometry was performed using a panel of monoclonal antibodies (4 colors). Results: Nonallergic asthma patients treated with high doses of inhaled FP showed a significant reduction in the percentages of CD3+ T lymphocytes compared to healthy controls. Untreated patients showed a significant increase in CCR6 expression in CD8+CD25+ and CD8+CD25+bright T cells compared to healthy controls. The results were similar for CXCR3 and CCR5 expression. In patients treated with standard doses of FP, CCR5 expression was significantly increased in CD3+ T lymphocytes relative to healthy controls. Conclusions: The different groups of clinically stable nonallergic asthmatic patients showed distinct patterns of alterations in subset distribution as well as CCR6, CXCR3, and CCR5 expression on circulating T lymphocytes.

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“…For instance, the presence of histamine during TLR4 and TLR3 activation of human mDCs leads to a reduced production of IL12p70, a Th1 polarizing cytokine, of CXCL10, a Th1 attracting chemokine, and increased release of IL10, IL8, or IL6 (10 -15). In another study it was reported that the presence of histamine during TLR3 stimulation of mDC down-regulates CCL1, TNF␣, IL6, and CXCL10, which is accompanied by up-regulation of CXCL1 (16). Furthermore, Frei et al (15) reported that histamine suppressed TLR4-induced TNF␣ secretion from mDC cell.…”

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confidence: 95%

“…In a specific experimental setting, where mDCs were stimulated with HIV-1 and histamine, T lymphocytes shifted toward FoxP3ϩ Treg (17). Among the many studies on human mDCs, few reported that histamine may determine F-actin polarization influencing the migratory ability of mDCs (13,16), and none investigated the specific histamine effects on actin cytoskeleton organization, which is a way to modulate the activity of mDCs, DC:T conjugate formation, and mDCs polarizing ability (18 -20).…”

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confidence: 99%

Histamine Regulates Actin Cytoskeleton in Human Toll-like Receptor 4-activated Monocyte-derived Dendritic Cells Tuning CD4+ T Lymphocyte Response

Aldinucci¹,

Bonechi²,

Manuelli³

et al. 2016

Journal of Biological Chemistry

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Histamine, a major mediator in allergic diseases, differentially regulates the polarizing ability of dendritic cells after Tolllike receptor (TLR) stimulation, by not completely explained mechanisms. In this study we investigated the effects of histamine on innate immune reaction during the response of human monocyte-derived DCs (mDCs) to different TLR stimuli: LPS, specific for TLR4, and Pam3Cys, specific for heterodimer molecule TLR1/TLR2. We investigated actin remodeling induced by histamine together with mDCs phenotype, cytokine production, and the stimulatory and polarizing ability of Th0. By confocal microscopy and RT-PCR expression of Rac1/CdC42 Rho GTPases, responsible for actin remodeling, we show that histamine selectively modifies actin cytoskeleton organization induced by TLR4, but not TLR2 and this correlates with increased IL4 production and decreased IFN␥ by primed T cells. We also demonstrate that histamine-induced cytoskeleton organization is at least in part mediated by down-regulation of small Rho GTPase CdC42 and the protein target PAK1, but not by down-regulation of Rac1. The presence and relative expression of histamine receptors HR1-4 and TLRs were determined as well. Independently of actin remodeling, histamine downregulates IL12p70 and CXCL10 production in mDCs after TLR2 and TLR4 stimulation. We also observed a trend of IL10 up-regulation that, despite previous reports, did not reach statistical significance.

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Histamine Downregulates the Th1-Associated Chemokine IP-10 in Monocytes and Myeloid Dendritic Cells

Cited by 19 publications

References 38 publications

International Union of Basic and Clinical Pharmacology. XCVIII. Histamine Receptors

International Union of Basic and Clinical Pharmacology. XCVIII. Histamine Receptors

Abnormal Chemokine Receptor Profile on Circulating T Lymphocytes from Nonallergic Asthma Patients

Histamine Regulates Actin Cytoskeleton in Human Toll-like Receptor 4-activated Monocyte-derived Dendritic Cells Tuning CD4+ T Lymphocyte Response

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