Histamine dilutions modulate basophil activation

Belon, Philippe; Cumps, Jean; Ennis, Madeleine; Mannaioni, P. F.; Roberfroid, Marcel; Sainte-Laudy, J.; Wiegant, F. A. C.

doi:10.1007/s00011-003-1242-0

Cited by 128 publications

(5 citation statements)

References 28 publications

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“…However, the pattern is much more complex since, in the LD test where the most significant data were observed, the 7C and 30C dilutions showed lower effects than 5C and 9C, respectively. A number of observations—coming from several research fields (Belougne-Malfatti et al 1998; Brown and Ennis 2001; Marotta et al 2003; Belon et al 2004; Eizayaga et al 2005; Bellavite et al 2006b; Witt et al 2007; Ruiz-Vega and Estevez-Delgado 2008; Chirumbolo et al 2009)—suggest that in high-dilution pharmacology, there is no linear or proportional relation between the molecular concentration of active substances and the therapeutic effect. Thus far there is no satisfactory or unifying theoretical explanation for these claims, though some hypotheses suggest a role of the nano-heterogeneous structures and dynamics of the solvent water (or water–ethanol) on a mesoscopic scale (Arani et al 1995; Del Giudice and Preparata 1998; Smith 2004) and/or of epitaxy (Demangeat et al 2004; Elia et al 2004, 2007; Mastrangelo 2007; Rao et al 2007; Chaplin 2007; Demangeat 2009).…”

Section: Discussionmentioning

confidence: 99%

Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice

et al. 2010

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IntroductionThis study was designed to investigate the putative anxiolytic-like activity of ultra-low doses of Gelsemium sempervirens (G. sempervirens), produced according to the homeopathic pharmacopeia.MethodsFive different centesimal (C) dilutions of G. sempervirens (4C, 5C, 7C, 9C and 30C), the drug buspirone (5 mg/kg) and solvent vehicle were delivered intraperitoneally to groups of ICR-CD1 mice over a period of 9 days. The behavioral effects were assessed in the open-field (OF) and light–dark (LD) tests in blind and randomized fashion.ResultsMost G. sempervirens dilutions did not affect the total distance traveled in the OF (only the 5C had an almost significant stimulatory effect on this parameter), indicating that the medicine caused no sedation effects or unspecific changes in locomotor activity. In the same test, buspirone induced a slight but statistically significant decrease in locomotion. G. sempervirens showed little stimulatory activity on the time spent and distance traveled in the central zone of the OF, but this effect was not statistically significant. In the LD test, G. sempervirens increased the % time spent in the light compartment, an indicator of anxiolytic-like activity, with a statistically significant effect using the 5C, 9C and 30C dilutions. These effects were comparable to those of buspirone. The number of transitions between the compartments of the LD test markedly increased with G. sempervirens 5C, 9C and 30C dilutions.ConclusionThe overall pattern of results provides evidence that G. sempervirens acts on the emotional reactivity of mice, and that its anxiolytic-like effects are apparent, with a non-linear relationship, even at high dilutions.

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Section: Discussionmentioning

confidence: 99%

Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice

et al. 2010

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“…Activation of mast cells via cross-linking of membrane bound IgE induces a release of potent mediators including histamine. Besides its pro-inflammatory effect, histamine exerts other regulatory functions by modulating the activity of T cells, monocytes, macrophages, eosinophils [5] and basophils [6]. In addition, histamine can inhibit further degranulation of mast cells by acting on H4 receptors [7], which suggests it is insolvent in the feed-back regulation of immunological responses.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Mast Cell Toll-Like Receptor 3 Expression and Cytokines Release by Histamine

Xie¹,

Wang²,

Peng³

et al. 2018

Cell Physiol Biochem

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Background/Aims: As a major inflammatory molecule released from mast cell activation, histamine has been reported to regulate TLRs expression and cytokine production in inflammatory cells present in the microenvironment. In this study, we determined the ability of histamine to modulate TLRs expression and cytokine production in mast cells. Methods: HMC-1 and P815 cells were exposed to various concentrations of histamine in the presence or absence of histamine antagonist for 2, 6 or 16 h. The effect of histamine on the expression of TLR3 protein and mRNA was analyzed by flow cytometry、 RT-PCR and immunofluorescent microscopy. Furthermore, we also examined the effect of histamine on the secretion of MCP-1 and IL-13 from mast cells by ELISA. Results: The amplification of TLR3 mRNA and protein expression in mast cells was observed after incubation with histamine, which was accompanied by increasing secretion of IL-13 and MCP-1 via H1 receptor. The signaling pathways of PI3K/ Akt and Erk1/2/MAPK contributed to these induction effects. Conclusion: These results demonstrate that histamine up-regulates the expression of TLR3 and secretion of IL-13 and MCP-1 in mast cells, thus identifying a new mechanism for the histamine inducing allergic response.

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“…6 , 148 , 149 The convergent clinical and basic science evidence, however, is not on the side of the skeptics in terms of the real-world utility of homeopathy. Indeed, the properties of NPs, 15 - 17 , 150 , 151 complex adaptive systems models for homeopathic medicine interactions with the individual patient; 84 , 85 , 88 , 92 , 94 , 108 and nonlinear doseresponse effects at low doses of substances 23 , 100 , 152 , 153 are also emerging as major frontiers in conventional medical research. 22 , 154 - 164 An appropriately conservative conclusion is that the effectiveness data are more extensive than the efficacy data for homeopathic medicines in AOM 129 , 141 and URIs to date.…”

Section: Discussionmentioning

confidence: 99%

Homeopathic Medications as Clinical Alternatives for Symptomatic Care of Acute Otitis Media and Upper Respiratory Infections in Children

Bell

Boyer²

2013

Glob Adv Health Med

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The public health and individual risks of inappropriate antibiotic prescribing and conventional over-the-counter symptomatic drugs in pediatric treatment of acute otitis media (AOM) and upper respiratory infections (URIs) are significant. Clinical research suggests that over-the-counter homeopathic medicines offer pragmatic treatment alternatives to conventional drugs for symptom relief in children with uncomplicated AOM or URIs. Homeopathy is a controversial but demonstrably safe and effective 200-year-old whole system of complementary and alternative medicine used worldwide. Numerous clinical studies demonstrate that homeopathy accelerates early symptom relief in acute illnesses at much lower risk than conventional drug approaches. Evidence-based advantages for homeopathy include lower antibiotic fill rates during watchful waiting in otitis media, fewer and less serious side effects, absence of drug-drug interactions, and reduced parental sick leave from work. Emerging evidence from basic and preclinical science research counter the skeptics' claims that homeopathic remedies are biologically inert placebos. Consumers already accept and use homeopathic medicines for self care, as evidenced by annual US consumer expenditures of $2.9 billion on homeopathic remedies. Homeopathy appears equivalent to and safer than conventional standard care in comparative effectiveness trials, but additional well-designed efficacy trials are indicated. Nonetheless, the existing research evidence on safety supports pragmatic use of homeopathy in order to “first do no harm” in the early symptom management of otherwise uncomplicated AOM and URIs in children.

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Histamine dilutions modulate basophil activation

Cited by 128 publications

References 28 publications

Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice

Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice

Modulation of Mast Cell Toll-Like Receptor 3 Expression and Cytokines Release by Histamine

Homeopathic Medications as Clinical Alternatives for Symptomatic Care of Acute Otitis Media and Upper Respiratory Infections in Children

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