Hispolon induces apoptosis in human gastric cancer cells through a ROS-mediated mitochondrial pathway

Chen, Wei; Zhao, Zhao; Li, Ling; Wu, Bin; Chen, Shifei; Zhou, Heling; Wang, Yong; Li, Yongquan

doi:10.1016/j.freeradbiomed.2008.03.013

Cited by 126 publications

(80 citation statements)

References 52 publications

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“…Hispolon was initially isolated as a yellow pigment from Inonotus hispidus and then identified in a number of traditional medicinal mushrooms, including Phellinus linteus and Phellinus igniarius, which have been used to treat various cancer types in East Asia (9)(10)(11). Hispolon has been reported to possess analgesic, anti-inflammatory and anticancer activities (10,(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

“…Hispolon has been reported to possess analgesic, anti-inflammatory and anticancer activities (10,(12)(13)(14). A previous study demonstrated that hispolon may induce apoptosis in breast and bladder cancer cells via the MDM2-recruited ERK1/2 activity, and downregulate the level of MDM2 through the chaperone-mediated autophagy pathway (15,16).…”

Section: Introductionmentioning

confidence: 99%

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Hispolon inhibits TPA-induced invasion by reducing MMP-9 expression through the NF-κB signaling pathway in MDA-MB-231 human breast cancer cells

2015

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Abstract. Hispolon has been demonstrated to possess analgesic, anti-inflammatory and anticancer activities. However, whether hispolon prevents the invasion of breast carcinoma cells and the underlying mechanisms of its action remain unknown. In the present study, various assays, including a matrigel-based Transwell invasion assay and electrophoretic mobility shift assay, were used to investigate the anti-invasion effect of hispolon and explore its mechanism of action. The results revealed that hispolon inhibited the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced migration and invasion of MDA-MB-231 human breast cancer cells at non-toxic concentrations. Hispolon also prevented the TPA-induced secretion of matrix metalloproteinase-9 (MMP-9) and reduced its expression at the transcriptional and translational levels. Furthermore, the phosphorylation of IκBα was reduced by hispolon, which resulted in the suppression of nuclear factor-κB (NF-κB), and p65 phosphorylation and nuclear translocation. An electrophoretic mobility shift assay demonstrated that NF-κB DNA-binding activity was induced by TPA and inhibited by hispolon. In addition, Bay 11-7082, which is a specific inhibitor of NF-κB, functioned in a similar manner as hispolon and blocked the secretion and expression of MMP-9. In conclusion, the results of the present study indicated that hispolon inhibited TPA-induced migration and invasion of MDA-MB-231 cells by reducing the secretion and expression of MMP-9 through the NF-κB signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hispolon inhibits TPA-induced invasion by reducing MMP-9 expression through the NF-κB signaling pathway in MDA-MB-231 human breast cancer cells

2015

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“…38) Many studies have shown that more cancer cells tend to get damaged by additional oxidative stress than normal cells. 25,39) The hydroperoxy group might be the reason for selective cytotoxicity of cordycepol C to cancer cells. Considering its unique structure and the ability to selectively kill tumor cells, cordycepol C may be a potential lead for future drug development.…”

Section: Discussionmentioning

confidence: 99%

“…Cell Viability Assay The cell viability was measured by the MTT method as previously described. 25) Briefly, cells were seeded in 96-well microtiter plates at a density of 5×10 3 cells/well for 24 h. After the indicated concentrations of drug treatment for 24 h, cells were incubated with MTT (0.5 mg/ mL) for an additional 4 h at 37°C. The resulting formazan precipitate was dissolved in 150 µL DMSO and the absorbance was detected at 490 nm with a Sunrise microplate reader (Tecan Group Ltd., Seestrasse, Switzerland).…”

Section: Cell Culture and Reagentsmentioning

confidence: 99%

Cordycepol C Induces Caspase-Independent Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells

Sun

Zhao

et al. 2014

Biological & Pharmaceutical Bulletin

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Cordycepol C, a novel sesquiterpene isolated from the cultured mycelia of Cordyceps ophioglossoides, contains a hydroperoxy group and is cytotoxic to HepG2 cells. So far, no sesquiterpenes have been found in the genus Cordyceps and it would be interesting to investigate the antitumor efficacy as well as the mechanism of action of this unusual sesquiterpene. In this study, we showed that cordycepol C induced apoptosis of the HepG2 cells without affecting the normal liver cell line L-02. Cordycepol C caused poly(ADP-ribose) polymerase-1 (PARP-1) cleavage and triggered the loss of mitochondrial membrane potential (Δψ m ) in HepG2 cells in a time-and dose-dependent manner, resulting in the nuclear translocation of apoptosis-inducing factor (AIF) and endonuclease G (Endo G). We also found that cordycepol C induced the expression of Bax protein, followed by its translocation from the cytosol to mitochondria in both wild type and p53 knockdown HepG2 cells. However, cordycepol C could not cause cleavages of procaspase-3, -8, and -9. Caspase activities were not increased and Z-VAD-fmk, a caspase inhibitor, could not prevent the apoptosis induced by cordycepol C. These findings indicate that cordycepol C induces caspase-independent apoptosis in HepG2 cells through a p53-independent and Bax-mediated mitochondrial pathway, leading to the nuclear translocation of AIF and Endo G. Our study provides the molecular mechanism by which cordycepol C induces apoptosis in hepatocellular carcinoma cells and indicates the potential use of cordycepol C as an antitumor agent.

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“…In addition, HPL has demonstrated antitumor effects in various types of cancer cells, including melanoma, leukemia, hepatocarcinoma, bladder cancer and gastric cancer cells (16)(17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Hispolon as an inhibitor of TGF-β-induced epithelial-mesenchymal transition in human epithelial cancer cells by co-regulation of TGF-β-Snail/Twist axis

et al. 2017

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Abstract. Hispolon (HPL), isolated from Phellinus linteus, has been used to treat various types of pathology, including inflammation, gastroenteric disorders, lymphatic diseases and numerous cancer subtypes. HPL has previously been reported to demonstrate a significant therapeutic efficacy against various types of cancer cells, including melanoma, leukemia, hepatocarcinoma, bladder and gastric cancer cells. However, its potential role in the epithelial-mesenchymal transition (EMT) has not been demonstrated. The present study investigated the effects of HPL on the EMT. Transforming growth factor β (TGF-β) induced enhanced cell migration and invasion, EMT-associated phenotypic changes. In the present study, HPL recovered the reduction of E-cadherin expression level in TGF-β treated cancer cells, which was regulated by the expression of Snail and Twist. HPL downregulated Snail and Twist, an effect that was enhanced by TGF-β. These findings provide novel evidence that HPL suppresses cancer cell migration and invasion by inhibiting EMT. Therefore, HPL may be a potent anticancer agent, inhibiting metastasis.

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Hispolon induces apoptosis in human gastric cancer cells through a ROS-mediated mitochondrial pathway

Cited by 126 publications

References 52 publications

Hispolon inhibits TPA-induced invasion by reducing MMP-9 expression through the NF-κB signaling pathway in MDA-MB-231 human breast cancer cells

Hispolon inhibits TPA-induced invasion by reducing MMP-9 expression through the NF-κB signaling pathway in MDA-MB-231 human breast cancer cells

Cordycepol C Induces Caspase-Independent Apoptosis in Human Hepatocellular Carcinoma HepG2 Cells

Hispolon as an inhibitor of TGF-β-induced epithelial-mesenchymal transition in human epithelial cancer cells by co-regulation of TGF-β-Snail/Twist axis

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