HiSorb sorptive extraction for determining salivary short chain fatty acids and hydroxy acids in heart failure patients

Lenzi, Alessio; Biagini, Denise; Ghimenti, Silvia; Vivaldi, Federico; Salvo, Pietro; Francesco, Fabio Di; Lomonaco, T

doi:10.1016/j.jchromb.2023.123826

Cited by 4 publications

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Disease-speci c VOCs are produced mainly through changes in speci c biochemical pathways in the body [11,12]. Following their production, VOCs are emitted and can, therefore, be found in in a variety of matrices, including: 1) infected cells and/or their microenvironment, 2) blood, 3) breath, 4) skin, 5) urine, 6) feces, and/or 7) saliva [13][14][15][16][17]. Research indicates that the VOCs in body cover a range of chemical families: acids, alcohols, ketones, aldehydes, amines, N-heterocycles, O-heterocycles, sulfur compounds, and hydrocarbons [13,18].…”

Section: Introductionmentioning

confidence: 99%

Volatile organic compounds in urine reveals distinct diagnostic signatures for gastric cancer

Sha,

Fei,

Zhao

et al. 2024

Preprint

View full text Add to dashboard Cite

Introduction: Gastric cancer (GC) remains a significant contributor to cancer-related mortality, underscoring the critical necessity for specific biomarkers to enable early diagnosis and prognosis. Analyzing volatile organic compounds (VOCs) in vivo offers a promising non-invasive approach for assessing metabolic processes. Methods A total of 201 metabolic samples were acquired from 63 GC patients and 65 healthy controls. Employing solid-phase microextraction and gas chromatography-ion mobility spectrometry-based analytical procedures, we conducted qualitative and signal response analysis of VOCs in blood, feces and urine. Volatolomics was comprehensively investigated across multiple human matrices, and a machine learning-based marker importance assessment framework was employed to evaluate diagnostic biomarkers of GC. Furthermore, a single urine test diagnostic method was established to assess the sensitivity and accuracy of VOCs in diagnosing GC. Results We underscored the specific VOCs alterations in human matrices, with particular emphasis on serum, feces and urine. We confirmed the dysregulation of GC metabolism during tumor development, as evidenced by VOCs such as short-chain fatty acids and ketones. Our developed urine-based VOCs targeted assay demonstrated superior diagnostic efficacy (AUC = 0.85, accuracy = 0.76, precision = 0.78, sensitivity = 0.75, F1 score = 0.75) compared to conventional serum markers (AUC = 0.68, accuracy = 0.63, precision = 0.70, sensitivity = 0.72, F1 score = 0.69). Conclusions Urine VOCs testing enhances GC detection efficacy and represents a novel strategy for cancer diagnosis. The confirmed robustness and precision underscore its potential for clinical translation. Trial registration ChiCTR, ChiCTR2300073117. Registered 2 July 2023 Retrospectively registered, https//www.chictr.org.cn/showproj.html?proj=200842

show abstract