Hirschsprung's disease: A clinical and pathologic overview

Blisard, Karen S.; Kleinman, Russell

doi:10.1016/s0046-8177(86)80559-7

Cited by 22 publications

(1 citation statement)

References 13 publications

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“…The clinical information about the exact site of rectal biopsy proved to be of crucial importance for the correct diagnosis of hypoganglionosis, regardless of the method used. First, because specimens might be taken from the usual hypoganglionic zone of the rectum (1.5-2.0 em above the pectinate line), and , second, because the hypoganglionic area near the aganglionic segment in HD could yield a falsepositive diagnosis of hypoganglionosis (6,18,19). Although denied by a few authors as existing as a discrete entity (9), hypoganglionosis is an uncommon disease defined by a decreased number of intramural neurons together with hypertrophic nerve trunks .…”

Section: Discussionmentioning

confidence: 99%

Usefulness of Immunocytochemical Demonstration of Neuron‐Specific Enolase in the Diagnosis of Hirschsprung's Disease

M. F. Nogueira,

Barbosa,

Carvalho

et al. 1990

J. pediatr. gastroenterol. nutr.

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The work reported here was carried out to study the importance of immunocytochemical staining of neuron‐specific enolase (NSE) in the diagnosis of Hirschsprung's disease and to compare its results with those obtained by hematoxylin‐eosin (H&E) staining in consecutive sections. A retrospective study was made on 51 rectal mucosal biopsies and 19 colorectal surgical specimens from 52 patients clinically suspected of Hirschsprung's disease. Several consecutive sections from all cases were restained by H&E and for NSE demonstration. Sixteen (31%) patients had a histological diagnosis of Hirschsprung's disease, 9 (17%) had hypoganglionosis, 4 (8%) had neuronal intestinal dysplasia, and 2 (4%) had normal histology. In eight patients (15%) hypoganglionosis remained dubious, and in 10 (19%) the diagnosis was inconclusive. Although the NSE staining improved the identification of the nervous tissue of the colon, both H&E and NSE staining proved to be of equal value in the assessment of the presence of neurons in the rectal wall of people with clinically suspected cases of Hirschsprung's disease. Ten H&E‐stained sections from different levels of the biopsy specimen would be enough to detect ganglion cells in most cases.

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Section: Discussionmentioning

confidence: 99%