Hippophae rhamnoides mediate gene expression profiles against keratinocytes infection of Staphylococcus aureus

Shah, Humaira; Shakir, Hafiz Abdullah; Safi, Sher Zaman; Ali, Abid

doi:10.1007/s11033-021-06221-3

Cited by 5 publications

(8 citation statements)

References 55 publications

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“…The cytotoxicity of the synthesized kirenol derivatives against cultured RAW 264.7 macrophage cells was assessed using the CCK-8 assay. The RAW 264.7 cells were incubated with different concentrations (5,10,25,50, and 100 μM) of kirenol derivatives for 24 h. The results shown in Table 1S (see supporting information) confirmed that most of the derivatives had no adverse effects on the viability of RAW264.7 cells when supplied at concentrations below 100 μM. However, compounds 3 f, 6 f, 14, and 16 c significantly reduced cell viability at a concentration of 50 μM, while compound 16 b demonstrated significant reduction in cell viability at a concentration of 25 μM.…”

Section: Effect Of Kirenol Derivatives On the Cell Viability Of Raw26...mentioning

confidence: 99%

“…The medium was routinely changed every day. [25] The anti-inflammatory effects of kirenol derivatives were investigated in lipopolysaccharide (LPS)-induced RAW 264.7 cells. 5 × 10 5 RAW264.7 cells were cultured in each well of a 96 well plate for 24 h. Tested compounds were dissolved at indicated concentrations (50, 25, 12.5, 6.25 and 3.125 μM/L) in DMSO, and then further diluted in DMEM, so that the final concentration of DMSO in the medium was 0.2 %.…”

Section: Raw 2647 Cell Culture and Treatmentmentioning

confidence: 99%

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Design, Synthesis, and Evaluation of Kirenol Derivatives as MMP‐8 Inhibitors with Anti‐inflammatory Activity

Zhao

Liu

et al. 2023

ChemistrySelect

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The diterpene kirenol has notable biological activity. In this study, molecular docking software (Discovery Studio) was adopted to investigate the anti‐inflammatory and antioxidant properties of kirenol. Twenty‐nine kirenol derivatives were synthesized based on the molecular docking outcomes. Cytotoxicity analyses of the kirenol derivatives were performed in Raw264.7 macrophages using the CCK‐8 assay, and nitric oxide (NO) concentrations were determined using the Griess assay. The DCFH‐DA approach identified the general pattern of reactive oxygen species (ROS) fluctuations. The mitochondrial mass and morphology were detected using the Mito‐Tracker Red fluorescent probe. Preliminary bioassay tests indicated that the concentration of NO was substantially reduced by certain derivatives. Moreover, these derivatives inhibited LPS‐induced ROS generation, and one of them exhibited a further reducing ROS generation and pro‐inflammatory response by suppressing excessive mitochondrial fission. These findings suggest that this particular derivative may be a promising natural compound for combating oxidative inflammatory diseases.

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Section: Effect Of Kirenol Derivatives On the Cell Viability Of Raw26...mentioning

confidence: 99%

Section: Raw 2647 Cell Culture and Treatmentmentioning

confidence: 99%

Design, Synthesis, and Evaluation of Kirenol Derivatives as MMP‐8 Inhibitors with Anti‐inflammatory Activity

Zhao

Liu

et al. 2023

ChemistrySelect

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“…Sea buckthorn leaf extract at a 5% concentration inhibited S. aureus , S. epidermidis , S. intermedius , and S. pyogenes growth by almost 50%. Sea buckthorn extract may reverse the detrimental effects of S. aureus on human keratinocytes by down-regulating various pro-inflammatory cytokines and apoptotic pathways, such as ILs, TNFs, transforming growth factors (TGFs), IFNs, fibroblast growth factors (FGFs), MAPKs, matrix metalloproteinases (MMPs), and caspases and Wnts molecular pathways ( 81 ). Additionally, a study showed that 6 mg/mL of sea buckthorn berry and leaf extract significantly inhibits the growth of Methicillin-resistant S. aureus (MRSA) ( 82 ).…”

Section: Health Benefitsmentioning

confidence: 99%

Phytochemistry, health benefits, and food applications of sea buckthorn (Hippophae rhamnoides L.): A comprehensive review

et al. 2022

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Sea buckthorn (Hippophae rhamnoides L.), an ancient miraculous plant, is of great interest because of its tenacity, richness in nutritional active substances, and biological activity. Sea buckthorn is a deciduous shrub or tree of the genus Hippophae in the family Elaeagnaceae. It is a pioneer tree species for soil improvement, wind and sand control, and soil and water conservation. Sea buckthorn contains many nutritional active components, such as vitamins, carotenoids, polyphenols, fatty acids, and phytosterols. Moreover, sea buckthorn has many health benefits, such as antioxidant, anticancer, anti-hyperlipidemic, anti-obesity, anti-inflammatory, antimicrobial, antiviral, dermatological, neuroprotective, and hepatoprotective activities. Sea buckthorn not only has great medicinal and therapeutic potential, but also is a promising economic plant. The potential of sea buckthorn in the human food industry has attracted the research interest of researchers and producers. The present review mainly summarizes the phytochemistry, nutrients, health benefits, and food applications of sea buckthorn. Overall, sea buckthorn is a dietary source of bioactive ingredients with the potential to be developed into functional foods or dietary supplements for the prevention and treatment of certain chronic diseases, which deserves further research.

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“…Hippophae rhamnoides L. is a deciduous shrub of the family Hippophae in Elaeagnaceae , native to Eurasian Mainland, especially the northwest of China [1] . The fruits, leaves, and seeds of H. rhamnoides were widely used.…”

Section: Introductionmentioning

confidence: 99%

“…Hippophae rhamnoides L. is a deciduous shrub of the family Hippophae in Elaeagnaceae, native to Eurasian Mainland, especially the northwest of China. [1] The fruits, leaves, and seeds of H. rhamnoides were widely used. Among them, H. rhamnoides fruits contain many bioactive fractions, such as polysaccharides, flavonoids, organic acids, fatty acids, and polyphenols.…”

Section: Introductionmentioning

confidence: 99%

Flash Extraction, Characterization, and Immunoenhancement Activity of Polysaccharide fromHippophae rhamnoidesLinn

Zhu

et al. 2023

Chemistry & Biodiversity

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Hippophae rhamnoides L. polysaccharide was optimized with flash extraction by response surface design. The optimum process conditions were: rotation rate 5000 r/min, extraction time 15 s, extraction temperature 90 °C and liquid-to-material ratio 38 mL/g, the extraction yield was 15.28 � 0.02 %. HRP-1 and HRP-2 obtained by 40 % and 60 % graded alcohol precipitation were characterized. The results indicated that HRP-1 and HRP-2 both composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose with different molar ratio and the molecular weights were 380.59 kDa and 288.24 kDa, respectively. In addition, the in vitro antioxidant and immunoenhancement activities of HRP-1 and HRP-2 were analyzed, and the two fractions showed good free radical scavenging activity against * OH, ABTS * + , DPPH * , and extremely strong immunomodulatory activity against RAW264.7 cells. Indicating that flash extraction is suitable for extraction of HRP, the structural study of HRP provides a scientific theoretical basis for the development of Hippophae rhamnoides.

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Hippophae rhamnoides mediate gene expression profiles against keratinocytes infection of Staphylococcus aureus

Cited by 5 publications

References 55 publications

Design, Synthesis, and Evaluation of Kirenol Derivatives as MMP‐8 Inhibitors with Anti‐inflammatory Activity

Design, Synthesis, and Evaluation of Kirenol Derivatives as MMP‐8 Inhibitors with Anti‐inflammatory Activity

Phytochemistry, health benefits, and food applications of sea buckthorn (Hippophae rhamnoides L.): A comprehensive review

Flash Extraction, Characterization, and Immunoenhancement Activity of Polysaccharide fromHippophae rhamnoidesLinn

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