Hippocampus bcl-2 and bax Expression and Neuronal Apoptosis After Moderate Hypothermic Cardiopulmonary Bypass in Rats

Zhang, Ting Jie; Hang, Jian; Wen, Ding; Hang, Yan Nan; Sieber, Frederick E.

doi:10.1213/01.ane.0000199221.96250.8c

Cited by 33 publications

(31 citation statements)

References 20 publications

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“…The Western blot results showed that TAT-NBD prevented cytochrome c release from the mitochondria to the cytoplasm; meanwhile, expression of the mitochondrial Bax protein was down-regulated, whereas the mitochondrial Bcl-2 protein was up-regulated in the TAT-NBD-treated rats at 24 h after model establishment. The ratio of the proapoptotic (Bax)/ antiapoptotic (Bcl-2) protein is a crucial factor that determines whether cells undergo apoptosis (39). Therefore, the results suggested that the protective role of TAT-NBD in the rat kernicterus model may be mediated by interfering with the mitochondrial apoptotic pathway.…”

Section: Protective Role Of Nf-b Inhibition In Kernicterusmentioning

confidence: 97%

The Blockade of NF-κB Activation by a Specific Inhibitory Peptide Has a Strong Neuroprotective Role in a Sprague-Dawley Rat Kernicterus Model

Song

et al. 2015

Journal of Biological Chemistry

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Kernicterus, the permanent nerve damage occurring as a result of bilirubin precipitation, still occurs worldwide and may lead to death or permanent neurological impairments. However, the underlying mechanisms remain unclear, and effective therapeutic strategies are lacking. The present study aims to investigate the activation of NF-B and to identify the effect of NF-B inhibition on the newborn rat kernicterus model. The NF-B essential modifier-binding domain peptide (NBD), coupled with the HIV trans-activator of transcription peptide (TAT) was used to inhibit NF-B. NF-B was significantly activated in the cerebrum at 1 and 3 h (p < 0.05) after the model was established, as measured by EMSA. NF-B activation was inhibited by intraperitoneal administration of TAT-NBD. The general conditions of the TAT-NBD-treated rats were improved; meanwhile, these rats performed much better on the neurological evaluation, the rotarod test, and the Morris water maze test (p < 0.05) than the vehicle-treated rats at 28 days. Furthermore, the morphology of the nerve cells was better preserved in the TAT-NBD group, and these cells displayed less neurodegeneration and astrocytosis. Simultaneously, apoptosis in the brain was attenuated, and the levels of the TNF-␣ and IL-1␤ proteins were decreased (p < 0.01). These results suggested that NF-B was activated, and inhibition of NF-B activation by TAT-NBD not only attenuated the acute neurotoxicity, apoptosis, and inflammation, but also improved the long term neurobehavioral impairments in the kernicterus model rats in vivo. Thus, inhibiting NF-B activation might be a potential therapeutic approach for kernicterus.

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Section: Protective Role Of Nf-b Inhibition In Kernicterusmentioning

confidence: 97%

The Blockade of NF-κB Activation by a Specific Inhibitory Peptide Has a Strong Neuroprotective Role in a Sprague-Dawley Rat Kernicterus Model

Song

et al. 2015

Journal of Biological Chemistry

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“…Sections were incubated with a monoclonal antibody against Bax protein (Dako, Carpinteria, California, USA) [18] and glial fibrillary acidic protein (GFAP; Sigma, St Louis, Missouri, USA) [19]. Detection of the antibody was carried out using a biotin-streptavidin detection system with 3-amino 9-ethyl carbazole as a chromogen (Dako) for Bax and with 0.05% diaminobenzidine as a chromogen (Amersham, Little Chalfont, UK) for GFAP.…”

Section: Immunohistochemical Studymentioning

confidence: 99%

Effect of streptozotocin-induced diabetes mellitus on the cerebellar cortex of adult male albino rats

Selim

2013

The Egyptian Journal of Histology

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“…4,5 As apoptosis is one of the final fates of neurons in neurological injuries, more immediate intervention to prevent apoptosis may be valuable during the early postcardiopulmonary bypass (CPB) recovery period. 6 Neuregulin 1 (NRG1) is a secreted trophic factor with an epidermal growth factor-like domain, and is part of a family of more than 15 transmembrane proteins whose expression has been widely studied in the central nervous system (CNS), cardiovascular, respiratory, and immune systems. 7 NRG1 is reputed to improve neurological function after ischemic cerebral injuries, including focal stroke and transient middle cerebral artery occlusion and reperfusion.…”

Section: Introductionmentioning

confidence: 99%

Neuregulin 1 Attenuates Neuronal Apoptosis Induced by Deep Hypothermic Circulatory Arrest Through ErbB4 Signaling in Rats

Wang

et al. 2015

Journal of Cardiovascular Pharmacology

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Mounting evidence suggests that neurological injury occurs after deep hypothermic circulatory arrest (DHCA), a protocol widely used in surgery for congenital heart diseases and aortic repair. Neuregulin 1 (NRG1), a neurotrophic factor highly expressed in the central nervous system, is crucial for neuronal survival. However, whether NRG1 is protective against apoptosis induced by DHCA is still unclear, as are the putative mechanisms involved. In this study, exogenous human NRG1 pretreatment (2.5 and 3.75 ng/kg, intracarotid injection) significantly inhibited neuronal apoptosis in DHCA-treated male rats, and notably, endogenous NRG1 expression was also increased. Bcl-2, as well as phosphorylated phosphatidylinositol-3-kinase, Akt, and cAMP-response element binding protein, were all increased, resulting in phosphorylation and subsequent activation of the ErbB4 receptor. Finally, expression of the apoptosis-related protein cleaved-caspase-3 was decreased, resulting in the inhibition of neuronal apoptosis induced by DHCA. Thus, our data indicate that NRG1 treatment inhibited DHCA-induced neuronal apoptosis by activating ErbB4 signaling, providing a potential therapeutic pathway for the prevention of neurological injury induced by DHCA.

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Hippocampus bcl-2 and bax Expression and Neuronal Apoptosis After Moderate Hypothermic Cardiopulmonary Bypass in Rats

Cited by 33 publications

References 20 publications

The Blockade of NF-κB Activation by a Specific Inhibitory Peptide Has a Strong Neuroprotective Role in a Sprague-Dawley Rat Kernicterus Model

The Blockade of NF-κB Activation by a Specific Inhibitory Peptide Has a Strong Neuroprotective Role in a Sprague-Dawley Rat Kernicterus Model

Effect of streptozotocin-induced diabetes mellitus on the cerebellar cortex of adult male albino rats

Neuregulin 1 Attenuates Neuronal Apoptosis Induced by Deep Hypothermic Circulatory Arrest Through ErbB4 Signaling in Rats

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