Hippocampal neurodegeneration in experimental autoimmune encephalomyelitis (EAE): potential role of inflammation activated myeloperoxidase

Sajad, Mir; Zargan, Jamil; Chawla, Raman; Umar, Sadiq; Sadaqat, Mir; Khan, Haider Ali

doi:10.1007/s11010-009-0088-3

Cited by 57 publications

(30 citation statements)

References 29 publications

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“…This oligodendrocyte consumption is the explanation of the early neurological onset of MS, characterised by functional disconnection of CNS. Our study, and also studies of other authors, have shown that this lysis can be prevented by the treatment with antagonists of NO production and oxidative species scavengers [15,16,19,25]. These modulators regulated LP intensity regulate the neurological expression of EAE, although it is not a solitary protection mechanism.…”

Section: Suppression Of the Lipid Peroxidation Process In The Cns Redsupporting

confidence: 74%

Original article Suppression of the lipid peroxidation process in the CNS reduces neurological expression of experimentally induced autoimmune encephalomyelitis

Ljubisavljević¹,

Stojanović²,

Pavlović³

et al. 2013

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Suppression of the lipid peroxidation process in the CNS reduces neurological expression of experimentally induced autoimmune encephalomyelitis S Sr rd dj ja an n L Lj ju ub bi is sa av vl lj je ev vi ic c A b s t r a c t O Ob bj je ec ct ti iv ve e: : Here we report the influence of malondialdehyde (MDA) as a measure of the lipid peroxidation process (LP), on multiple sclerosis (MS) pathogenesis and its neurological signs, during the treatment with aminoguanidine (AG) -a selective inducible nitric oxide synthase inhibitor and N-Acetyl cysteine (NAC) -an oxidative scavenger, in the experimental autoimmune encephalomyelitis (EAE), an animal model for studying MS. M Ma at te er ri ia al l a an nd d m me et th ho od ds s: : Encephalomyelitis induction by the subcutaneous injection of myelin basic protein of bovine type, dissolved in phosphate buffered saline (PBS) emulsified in equal volume of the complete Freund's adjuvant (CFA), was described in detail in our earlier published papers. Each of animals was randomly assigned to seven groups -control (PBS), EAE, CFA, EAE + AG, AG, EAE

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Section: Suppression Of the Lipid Peroxidation Process In The Cns Redsupporting

confidence: 74%

Original article Suppression of the lipid peroxidation process in the CNS reduces neurological expression of experimentally induced autoimmune encephalomyelitis

Ljubisavljević¹,

Stojanović²,

Pavlović³

et al. 2013

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“…After the experiment, animals were sacrificed and the liver tissues were washed with PBS (pH 7.4) and placed on ice as method described earlier (Sajjad et al, 2009). Briefly, a 50 ml sample was added with 100 ml of Griess reagent and the reaction mixture was incubated for about 5-10 min at room temperature, protected from light.…”

Section: Measurement Of No: Griess Reactionmentioning

confidence: 99%

Anti-endotoxin effects of terpenoids fraction fromHygrophila auriculatain lipopolysaccharide-induced septic shock in rats

Hussain

Azam

Ahamed

et al. 2015

Pharmaceutical Biology

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Context: Hygrophila auriculata (K. Schum) Heine (Acanthaceae) has been traditionally used for the treatment of various ailments such as inflammation, rheumatism, jaundice and malaria. Objective: The present study aims to separate terpenoid fraction (TF) from alcohol (70%) extract of the whole plant of Hygrophila auriculata and assess its anti-inflammatory activity. Materials and methods: HPTLC analysis of TF was performed for the estimation of lupeol. Edema was induced in Wistar albino rats by subplanter injection of 0.1 ml of 1% (w/v) carrageenan into the right hind paw after 1 h of TF administration (100 and 200 mg/kg oral). Septic shock was induced by intraperitoneal administration of LPS (100 mg/kg) in rats and interleukins (IL-1b and IL-6), tumor necrosis factor (TNF-a), superoxide dismutase (SOD), lipid peroxidation (LPO), and nitric oxide (NO) were measured in serum. AutoDock 4.2 was used for molecular docking. Results: Administration of TF significantly (p50.005) restored the serum levels of cytokines, LPO (7.77 ± 0.034 versus 4.59 ± 0.059 nmole of TBARS), NO (9.72 ± 0.18 versus 4.15 ± 0.23 mmol nitrite/ mg of wet tissue), and SOD (4.89 ± 0.036 versus 7.83 ± 0.033 Unit/mg protein) compared with the LPS-challenged rats. Analysis of in silico results revealed that TNF-a is the most appropriate target in eliciting anti-inflammatory activity. Conclusion: The present findings suggest that TF of Hygrophila auriculata possesses great promise as an anti-inflammatory agent which may be due to its antioxidant effect. Molecular docking results could be exploited for lead optimization and development of suitable treatment of inflammatory disorders.

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“…24 Therefore, the possible factors for treating the disease must have the capacity to inhibit the deleterious mechanisms prompted by OS. 25 One of the common models for mimicking OS in vitro involves using H 2 O 2 and the pheochromocytoma (PC)-12 cell line.…”

Section: Introductionmentioning

confidence: 99%

The therapeutic effects of MSc1 nanocomplex, synthesized by nanochelating technology, on experimental autoimmune encephalomyelitic C57/BL6 mice

Fakharzadeh¹,

Sahraian²,

Hafizi³

et al. 2014

IJN

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Purpose Currently approved therapies for multiple sclerosis (MS) at best only slow down its progression. Therefore, it is necessary to utilize novel technologies in order to synthesize smart multifunctional structures. In the present study, for the first time we evaluated the therapeutic potential of MSc1 nanocomplex, which was designed based on novel nanochelating technology. Materials and methods MSc1 cell-protection capacity, with and without iron bond, was evaluated against hydrogen peroxide (H 2 O 2 )-induced oxidative stress in cultured rat pheochromocytoma-12 cells. The ability of MSc1 to maintain iron bond at pH ranges of 1–7 was evaluated. Nanocomplex toxicity was examined by estimating the intraperitoneal median lethal dose (LD 50 ). Experimental autoimmune encephalomyelitic mice were injected with MSc1 14 days after disease induction, when the clinical symptoms appeared. The clinical score, body weight, and disease-induced mortality were monitored until day 54. In the end, after collecting blood samples for assessing hemoglobin and red blood cell count, the brains and livers of the mice were isolated for hematoxylin and eosin staining and analysis of iron content, respectively. Results The results showed that MSc1 prevented H 2 O 2 -induced cell death even after binding with iron, and it preserved its bond with iron constant at pH ranges 1–7. The nanocomplex intraperitoneal LD 50 was 1,776.59 mg/kg. MSc1 prompted therapeutic behavior and improved the disabling features of experimental autoimmune encephalomyelitis, which was confirmed by decreased clinical scores versus increased body mass and 100% survival probability. It did not cause any adverse effects on hemoglobin or red blood cell count. Histopathological studies showed no neural loss or lymphocyte infiltration in MSc1-treated mice, while the hepatic iron content was also normal. Conclusion These results demonstrate that MSc1 could be a promising beneficial novel agent and has the capacity to be evaluated in further studies.

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Hippocampal neurodegeneration in experimental autoimmune encephalomyelitis (EAE): potential role of inflammation activated myeloperoxidase

Cited by 57 publications

References 29 publications

Original article Suppression of the lipid peroxidation process in the CNS reduces neurological expression of experimentally induced autoimmune encephalomyelitis

Original article Suppression of the lipid peroxidation process in the CNS reduces neurological expression of experimentally induced autoimmune encephalomyelitis

Anti-endotoxin effects of terpenoids fraction fromHygrophila auriculatain lipopolysaccharide-induced septic shock in rats

The therapeutic effects of MSc1 nanocomplex, synthesized by nanochelating technology, on experimental autoimmune encephalomyelitic C57/BL6 mice

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