Hippocampal microRNA-mRNA regulatory network is affected by physical exercise

Fernandes, Jansen; Vieira, André Schwambach; Soares, Juliana Carlota Kramer; Silva, Eduardo Alves da; Lee, Kil Sun; Lopes-Cendes, Íscia; Arida, Ricardo Mário

doi:10.1016/j.bbagen.2018.05.004

Cited by 27 publications

(14 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, We compared the EVs miRNAs through miRNA sequencing in sedentary and exercised mice; 46 miRNAs were upregulated and 36 downregulated in the exercise group. Among that, miR-10b-5p was upregulated in the rat hippocampus after 4 weeks of treadmill exercise (Fernandes et al, 2018 ) and miR-let-7i was downregulated in the plasma of athletes after exercise (Nielsen et al, 2014 ), consistent with our findings. Furthermore, as our results, the elevation of miR-342-5p in exercise-released exosome showed that reduced apoptosis and LDH release (a marker of tissue damage), increased cardiomyocyte viability (Hou et al, 2019 ).…”

Section: Discussionsupporting

confidence: 91%

Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety

Yoon

Park

Kwak

et al. 2021

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Anxiety disorders are the most frequently diagnosed psychological condition, associated with serious comorbidities including excessive fear and interference with daily life. Drugs for anxiety disorders are typically prescribed but the side effects include weight gain, nausea, and sleepiness. Exercise is an effective treatment for anxiety. Exercise induces the release of extracellular vesicles (EVs) into the circulation, which transmit signals between organs. However, the effects of exercise-induced EVs on anxiety remain poorly understood. Here, we isolated EVs from the sera of mice that were sedentary or that voluntarily exercised. We characterized the changes in the miRNA profile of serum EVs after 4 weeks of voluntary exercise. miRNA sequencing showed that 82 miRNAs (46 of which were positive and 36 negative regulators) changed after exercise. We selected genes affected by at least two miRNAs. Of these, 27.27% were associated with neurotrophin signaling (9.09% with each of central nervous system neuronal development, cerebral cortical cell migration, and peripheral neuronal development). We also analyzed behavioral changes in mice with 3 weeks of restraint stress-induced anxiety after injection of 20 μg amounts of EVs from exercised or sedentary mice into the left cerebral ventricle. We found that exercise-derived EVs reduced anxiety (compared to a control group) in a nest-building test but found no between-group differences in the rotarod or open field tests. Exercise-derived EVs enhanced the expression of neuroactive ligand-receptor interaction genes. Thus, exercise-derived EVs may exhibit anti-anxiety effects and may be of therapeutic utility.

show abstract

Section: Discussionsupporting

confidence: 91%

Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety

Yoon

Park

Kwak

et al. 2021

Front. Mol. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Recently mir-129 has also been associated with memory retention, in addition to its neuroprotective role on hypoxic-ischaemic brain injuries [39]. As shown by Fernandez et al [40] mir-129 and mir-144 play a major role in the modulation of the hippocampal microRNA-mRNA regulatory network upon physical exercise, which eventually lead to improvements in memory detention.…”

Section: Function-and Tissue-specific Expression Of Mirnamentioning

confidence: 96%

Deep sequencing of small non-coding RNA highlights brain-specific expression patterns and RNA cleavage

et al. 2019

View full text Add to dashboard Cite

With the advance of high-throughput sequencing technology numerous new regulatory small RNAs have been identified, that broaden the variety of processing mechanisms and functions of non-coding RNA. Here we explore small non-coding RNA (sncRNA) expression in central parts of the physiological stress and anxiety response system. Therefore, we characterize the sncRNA profile of tissue samples from Amygdala, Hippocampus, Hypothalamus and Adrenal Gland, obtained from 20 pigs. Our analysis reveals that all tissues but Amygdala and Hippocampus possess distinct, tissue-specific expression pattern of miRNA that are associated with Hypoxia, stress responses as well as memory and fear conditioning. In particular, we observe marked differences in the expression profile of limbic tissues compared to those associated to the HPA/stress axis, with a surprisingly high aggregation of 3´-tRNA halves in Amygdala and Hippocampus. Since regulation of sncRNA and RNA cleavage plays a pivotal role in the central nervous system, our work provides seminal insights in the role/involvement of sncRNA in the transcriptional and post-transcriptional regulation of negative emotion, stress and coping behaviour in pigs, and mammals in general.

show abstract

“…Therefore, We compared the EVs miRNAs through miRNA sequencing in sedentary and exercised mice; 46 miRNAs were upregulated and 36 downregulated in the exercise group. Among that, miR-10b-5p was upregulated in the rat hippocampus after 4 weeks of treadmill exercise (Fernandes et al, 2018) and miR-let-7i was downregulated in the plasma of athletes after exercise (Nielsen et al, 2014), consistent with our findings. Furthermore, as our results, the elevation of miR-342-5p in exercise-released exosome showed that reduced apoptosis and LDH release (a marker of tissue damage), increased cardiomyocyte viability (Hou et al, 2019).…”

Section: Target Scansupporting

confidence: 91%

Data_Sheet_1.xlsx

View full text Add to dashboard Cite

Hippocampal microRNA-mRNA regulatory network is affected by physical exercise

Cited by 27 publications

References 66 publications

Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety

Effects of Voluntary Running Wheel Exercise-Induced Extracellular Vesicles on Anxiety

Deep sequencing of small non-coding RNA highlights brain-specific expression patterns and RNA cleavage

Data_Sheet_1.xlsx

Contact Info

Product

Resources

About