Hippocampal damage induced by carbon monoxide poisoning and spreading depression is alleviated by chronic treatment with brain derived polypeptides

Koroleva, V. I.; Korolev, O.S.; Mareš, V; Pastalkova, Eva; Bureš, Jan

doi:10.1016/s0006-8993(98)01246-3

Cited by 12 publications

(6 citation statements)

References 35 publications

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“…As a reservoir of a large number of bioactive molecules, the PBE has been shown to have neuroprotective function in the hypoxic-induced pathological animal model and improve the brain functions following their administration [ 13 ]. The FGFs derived from the PBE has been well known to regulate proliferation and differentiation of MSCs into mature osteoblasts [ 10 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…The porcine brain, as a source of different growth factors including FGFs, has been traditionally regarded as food supplements to improve brain function by people in some areas of China and Thailand [ 12 ]. Moreover, the porcine brain derived peptides could increase the activities of various scavenger enzymes including superoxide dismutase, catalase and glutathione related enzymes for their potential neuroprotective effects [ 13 – 16 ]. Therefore, we introduced a method of collecting porcine brain extract (PBE) in this study, and investigated the effect of PBE on osteogenic differentiation of rat bone marrow derived mesenchymal stem cells (rBMSCs) and bone consolidation in a rat DO model.…”

Section: Introductionmentioning

confidence: 99%

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Porcine brain extract promotes osteogenic differentiation of bone marrow derived mesenchymal stem cells and bone consolidation in a rat distraction osteogenesis model

Sun

et al. 2017

PLoS ONE

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Distraction osteogenesis (DO) is the gold standard to treat large bone defects, but long consolidation period is a major limitation. Innovative efforts to promote osteogenesis are needed. Porcine brain extract (PBE) was reported to enhance the proliferation and differentiation of multiple primary cells. In this study, we aimed to develop a method for collecting PBE and investigate its effects on osteogenic differentiation of rat bone marrow derived mesenchymal stem cells (rBMSCs) and bone consolidation in a rat DO model. The PBE was collected from neonatal brain tissues of porcine fetus and was used to treat rBMSCs. Following PBE treatment (700 ng/ml), osteogenic differentiation was assessed. Further, we locally injected PBE (7 μg/ml, 100μl) or PBS (100μl) into the gap in a Sprague-Dawley (SD) male rat DO model every three days till termination. X-rays, micro-computed tomography, mechanical testing, histology and immunohischemistry examinations were used to exam the quality of the regenerates. The alkaline phosphatase, calcium deposits, and steogenic markers in the PBE treated rBMSCs were significantly increased. In the rat model, new bone properties of bone volume/total tissue volume and mechanical strength were higher in the PBE treated group. Histological analysis also confirmed more mineralized bone after PBE treatment. The current study reports a standard protocol for PBE collection and demonstrated its positive effects on osteogenic differentiation and bone consolidation in DO. Since the PBE is readily available and very cost effective, PBE may be a potential new bio-source to promote bone formation in patients undergo DO treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Porcine brain extract promotes osteogenic differentiation of bone marrow derived mesenchymal stem cells and bone consolidation in a rat distraction osteogenesis model

Sun

et al. 2017

PLoS ONE

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“…Recently, numerous studies including our previous study have demonstrated the neuroprotective and cognitive enhancing effects of the porcine brain-derived peptide preparation and porcine brain extract. In addition, the extract also increases the activities of various scavengering enzymes including superoxide dismutase, catalase and glutathione related enzymes (Koroleva et al, 1999;Schwab et al, 1998;Windisch et al, 1998;Gschanes et al, 1997;HutterPaier et al, 1996;Jittiwat et al, 2009). Based on the neuroprotective and antioxidant effects and the brain tonic reputation of porcine brain extract previously reported, we hypothesized that the porcine brain extract might mitigate memory impairment and neurodgeneration in animal model of Alzheimer's disease.…”

Section: Introductionmentioning

confidence: 86%

Neuroprotective Effect against Alzheimer's Disease of Porcine Brain Extract

Eduardo¹

2012

American Journal of Applied Sciences

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Problem statement: Despite the increasing importance of Alzheimer's disease, no effective therapeutic strategy is available. Therefore, neuroprotective strategy is still required. Recent findings show that numerous substances possessing antioxidant can improve neurodegeneration and memory impairment. Based on the antioxidant effect and its reputation to serve as brain tonic in traditional folklore, we hypothesized that porcine brain extract could mitigate neurodegeneration and memory impairment. Therefore, this study was set up to determine the effect of porcine brain extract on memory impairment and neurodegeneration in animal models of Alzheimer's disease. Approach: Male Wistar rats (180-220 g) had been orally given porcine brain extract at doses of 0.5 and 2.5 mg kg −1 BW for a period of 4 weeks before and 1 week after the induction of cognitive deficit condition as those found in early phase of Alzheimer's disease via the intraventricular injection of AF64A, a cholinotoxin. Rats were assessed the spatial memory using Morris water maze test. Then, they were determined neuron density in hippocampus using histological techniques. Moreover, the assessment of acetylcholinesterase (AChE) activity and malondialdehyde (MDA) level in hippocampus were also performed. Results: It was found that both doses of porcine brain extract could enhance memory, neuron and cholinergic neuron density in all subregions of hippocampus. In addition, the decreased AChE and MDA were also observed. Therefore, our results suggested that the possible underlying mechanism of the extract might occur partly via the decrease in oxidative stress marker, MDA and AChE. Conclusion: This study clearly demonstrates that porcine brain extract can protect against memory impairment and neurodegeneration in animal model of Alzheimer's disease. Therefore, it should be serve as the potential food supplement or adjuvant therapy against Alzheimer's disease and other age-related cognitive decline. However, further researches about therapeutic range and possible active ingredient are necessary.

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“…In deed, one such product, Cerebrolysin (Ebewe Pharmaceutical, Austria), has been in clinical application for more than 40 years. Its physiology activities have been confirmed by researchers all over the world [1][2][3][4][5][6]. It is used for the treatment of neural system diseases, especially Alzheimer's disease (AD) [7][8][9].…”

Section: Introductionmentioning

confidence: 96%

Analysis and determination of biological activity of short-chain peptides from porcine brain hydrolysate

Zhang

2005

Journal of Pharmaceutical and Biomedical Analysis

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Hippocampal damage induced by carbon monoxide poisoning and spreading depression is alleviated by chronic treatment with brain derived polypeptides

Cited by 12 publications

References 35 publications

Porcine brain extract promotes osteogenic differentiation of bone marrow derived mesenchymal stem cells and bone consolidation in a rat distraction osteogenesis model

Porcine brain extract promotes osteogenic differentiation of bone marrow derived mesenchymal stem cells and bone consolidation in a rat distraction osteogenesis model

Neuroprotective Effect against Alzheimer's Disease of Porcine Brain Extract

Analysis and determination of biological activity of short-chain peptides from porcine brain hydrolysate

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