Hiperlipidemia induzida por Triton WR-1339 (Tyloxapol) em Ratos Wistar

Bertges, Luiz Carlos; Mourão, Carlos Alberto; Souza, Jonathan Batista; Cardoso, Vinícius Antônio Coelho

doi:10.5935/2674-7960.v1-0003

Cited by 4 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Triton (Tyloxapol) is a non-anionic surfactant with a polymeric structure used in several studies to induce dyslipidemia in animal models. Its mechanisms of action are the ability to inhibit the enzyme lipoprotein lipase (LPL), responsible for the hydrolysis of triglycerides present in plasma lipoproteins, and the stimulation of hydroxymethyl glutaryl coenzyme A reductase (reductase), an enzyme involved in the biosynthesis of hepatic cholesterol (Bertges et al, 2011; Castro et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Cannabis sativa L. Fixed Oil and its Nanoemulsion: Effect on Diabetes and Dyslipidemia Induced in Rats

de Oliveira Carvalho,

de Melo Santos,

de Lima Teixeira dos Santos

et al. 2024

Pharmacognosy Magazine

View full text Add to dashboard Cite

Background: Diabetes Mellitus (DM) is a syndrome that interferes with energy metabolism and is caused by a decrease and dysfunction of insulin, leading to chronic hyperglycemia. DM associated with dyslipidemia is a severe health risk, increasing the chance of cardiovascular events, such as acute myocardial infarction and stroke. The Cannabis sativa L. fixed oil (CSO) is composed of unsaturated fatty acids and can be crucial in treating metabolic alterations. In addition, the nanoemulsion of C. sativa oil (NCS) has advantages in optimizing treatments. Objectives: This study aimed to evaluate the effects of treatments with CSO and its nanoemulsion (NCS) on induced diabetes and dyslipidemia in Wistar rats. Materials and methods: CSO’s physical-chemical and chromatographic characterization was performed, followed by the preparation of an NE containing 6% CSO. DM was induced in Wistar rats by intraperitoneal injection of streptozotocin (STZ) at a 55 mg/kg dose. Four days later, animals with blood glucose levels exceeding 300 mg/dL were considered diabetic. The rats were then divided into five groups ( n = 5) and treated orally. The groups included a normoglycemic control group (NOR), a diabetic control group, a group treated with metformin (100 mg/kg), a group treated with CSO (400 mg/kg), and a group treated with nanoemulsion (NCS 200 mg/kg). Subsequently, the pancreas’s clinical, biochemical, and histopathological parameters were evaluated. Results: In the chemical profile of CSO, it was observed the majority composition of palmitoleic (14.58%), oleic (12.50%), linoleic (42.40%), and linolenic (8.55%) acids. The results demonstrated that the induction of DM by STZ could reproduce the typical symptoms and clinical signs of DM. It was observed that treatments with CSO and NCS showed a significant improvement ( p < .001) in polydipsia, polyuria, and loss of body mass, as well as a significant reduction ( p < .001) of glucose levels in urine and blood and serum lipids. Histopathology of the pancreas revealed that treatment with CSO and NCS showed an increase in the number of cells in the islets of Langerhans and a decrease in regions devoid of cells, indicating possible cell regeneration. Moreover, insulin levels were significantly increased ( p < .05) in the preferred groups. With dyslipidemia induced by Triton (Tyloxapol), it was observed that the treatment with CSO and NCS significantly decreased the levels of triglycerides ( p < .05) and cholesterol ( p < .001), as well as low-density lipoproteins (LDL) ( p < .01). Conclusion: Treatment with CSO and NCS under the conditions of this study demonstrated an anti-diabetic effect and the ability to act in the reduction of triglyceride, cholesterol, and LDL levels. In this respect, treatments with CSO and NCS act in the control of DM, as well as in the prevention of cardiovascular diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Cannabis sativa L. Fixed Oil and its Nanoemulsion: Effect on Diabetes and Dyslipidemia Induced in Rats

de Oliveira Carvalho,

de Melo Santos,

de Lima Teixeira dos Santos

et al. 2024

Pharmacognosy Magazine

View full text Add to dashboard Cite

show abstract

“…Tyloxapol following intravenous or intra-peritoneal injection that causes the milky serum which last up to 48 hr ( Rasouli et al, 2016 ) as this is the acute model and its effect is abolished after 48 hr so no accumulation of cholesterol was observed and having no significant effect on endothelium ( Korolenko et al, 2010 ). After 48 hr this effect of tyloxapol is reversed that’s why it is used as an acute model of hyperlipedimia It causes a significant increase in hepatic cholesterol biosynthesis by stimulating the activity of HMG-CoA reductase ( Janicki and Aron, 1962 , Bertges et al, 2011 ). Previously it have been shown that Hibiscus rosa sinensis root at the dose of 500 mg/kg/day ( Kumar et al, 2009 ), Elaeis guineensis (250 and 500 mg/kg/day) ( Owolabi et al, 2013 ) also exhibit the lipid lowering effect in tylaxapol and olive oil induced hyperlipidemic rat models.…”

Section: Discussionmentioning

confidence: 99%

Onosma hispidum L. extract reverses hyperlipidemia, hypertension, and associated vascular dysfunction in rats

Wazir

Khan

Javed

et al. 2023

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

“…Triton WR-1339, a nonionic detergent, is frequently used to establish an acute hyperlipidemia model in animals to evaluate potential hyperlipidemic drugs (Bertges et al, 2011). It inhibits the uptake of circulating lipoproteins by extrahepatic tissues, resulting in a high plasma lipid profile and in increased blood lipoprotein levels (Da Rocha et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Walnut Seed Coat (Juglans regia L.), a Plant Effective in Human Health: Antioxidant Activity and in Rats Nephroprotective Effect

Palabıyık,

Uğuz,

Aşkın

et al. 2024

Research in Agricultural Sciences

View full text Add to dashboard Cite

In the study, the seed coat (WSC) of Posof (Ardahan/Türkiye) walnuts was extracted to determine their phytochemical components and antioxidant capacities. The effects of bioactive components in the ethanol extract of WSC (E-WSC) on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitors were investigated. Additionally, antioxidant enzyme activity parameters were measured in the kidney tissues of Triton WR-1339-induced hyperlipidemic rats. Bioactive compounds in WSC were identified by GC-MS system. The antioxidant properties of WSC were measured using Fe+3, Cu+2 and Fe+3-2,4,6-tripyridyl-s-triazine (TPTZ) reducing agent, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'- azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging activities. In this analysis, using 30 male Wistar rats (300 ± 30 g) randomly divided into five groups were treated as follows; K1: Healthy control group, K2: E-WSC (150 mg) o.d., K3: E-WSC (300 mg) o.d., K4: Hyperlipidemic group i.p., K5: Hyperlipidemic group i.p. + E-WSC (300 mg) o.d. Superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) analyzes were performed in kidney tissues. Based on these results, it was clearly determined that E-WSC has significant antioxidant activity due to its bioactive components, has an inhibitory effect on AChE and BChE enzymes, and has a protective effect against oxidative stress by improving hyperlipidemia-related kidney damage.

show abstract

Hiperlipidemia induzida por Triton WR-1339 (Tyloxapol) em Ratos Wistar

Cited by 4 publications

References 0 publications

Cannabis sativa L. Fixed Oil and its Nanoemulsion: Effect on Diabetes and Dyslipidemia Induced in Rats

Cannabis sativa L. Fixed Oil and its Nanoemulsion: Effect on Diabetes and Dyslipidemia Induced in Rats

Onosma hispidum L. extract reverses hyperlipidemia, hypertension, and associated vascular dysfunction in rats

Walnut Seed Coat (Juglans regia L.), a Plant Effective in Human Health: Antioxidant Activity and in Rats Nephroprotective Effect

Contact Info

Product

Resources

About