Hindered transmembrane protein transport in hollow-fibre devices

Effects of free convection on three‐dimensional protein transport in hollow‐fiber bioreactors

“…Their simulations of ECS protein transport in closed‐shell HFBRs showed that a neglect of the osmotic term would lead to unrealistic protein distributions, such as with all of the protein packed to an extremely high concentration within a very thin layer at the downstream end of the ECS. Subsequent experimental as well as theoretical investigations confirmed the importance of osmotic effects for a variety of HFBR protein transport conditions, both in closed‐shell [KCM studies by Patkar et al (1995), Koska et al (1997), and Łabȩcki et al (1998)] and in open‐shell configurations [PMM study by Łabȩcki et al (1996)].…”

“…As before, the ICS and the ECS of the hollow‐fiber bundle are treated as two interpenetrating porous media, with the governing equations formulated in a coupled form for both of these regions. In the present analysis, the membranes are assumed impermeable to protein, although the general model formulation does allow for transmembrane protein transport (Łabȩcki et al, 1998). Only the closed‐shell reactor configuration is considered.…”

“…The three‐dimensional distribution of protein in the ECS (with the neglect of dispersive fluxes arising from local velocity fluctuations) is described using a transient convection–diffusion equation, that is where C S is the local interstitial protein concentration (that is, mass of protein per unit volume of the ECS). For a leaking protein, equations derived elsewhere (Łabȩcki et al, 1998) are available to model the transmembrane flux, J TMP , and its effect on the ICS concentration. In the present analysis, the protein is assumed to be completely retained in the ECS or ICS, and hence J TMP is set to zero.…”

A mathematical model for release of biologics from porous hollow fibers

“…When considering protein transport through a hollow fiber membrane, the pore geometry of the membrane is an important consideration in modeling release. 12 Hollow fibers fabricated using a double injection nozzle often have both large macropores (Mp) as well as small micropores (lp), as a result of solvent extraction and spinodal decomposition 23 (Fig. 1, left).…”

“…In the separation technology field, large volumes can be filtered through the fibers, while utilizing minimal space and low power consumption. 12,13 In these examples, the composition of the solution that externally enters the system (inlet feed control) as well as the properties of the porous, hollow fiber can be manipulated to control the exchange process.…”